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Conference Paper: Adipocytes regulate osteogenesis in a novel lipoatrophic mouse model

TitleAdipocytes regulate osteogenesis in a novel lipoatrophic mouse model
Authors
Issue Date2020
PublisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org/
Citation
The 25th Annual Medical Research Conference, Hong Kong, 18 January 2020. In Hong Kong Medical Journal, 2020, v. 26 n. 1, Suppl. 1, p. 28, abstract no. 43 How to Cite?
AbstractIntroduction: Substantial clinical evidence demonstrating a complex relationship between adiposity and osteoporosis suggests that adipose tissue plays an important role in bone metabolism. However, the exact function of adipocytes and mechanisms underlying its impact on bone health are yet incompletely understood due to marked discrepancies in human and animal studies. In this study, the effect of adipocytes on osteogenesis was explored using a novel lipodystrophic mouse model. Methods: We generated adipose tissue-specific murine double minute 2 knockout (KO) mice with Cre/loxP system. Knockout mice displayed gradually loss of peripheral adipose tissue and bone marrow adipocytes until nearly 80% to 100% of total fat reduction at 12-week-old mice. Results: Micro-computed tomography revealed that bone mineralisation density of femur from 12-week-old KO mice was significantly higher than the wild-type (WT) mice. Interestingly, the absence of adipocytes in KO mice exhibited markedly lower serum levels of C-terminal telopeptides of type I collagen (bone resorption marker) than WT mice, whereas the N-terminal propeptides of type I collagen (bone formation marker) remained unaffected. No differences were observed in the ex vivo differentiation of osteoblasts and osteoclasts between WT and KO mice. In addition, adipocyte-conditioned media treatment of isolated haematopoietic stem cells from bone marrow enhanced the differentiation of osteoclasts. Conclusion: These results indicate that secreted factors from adipocytes may promote osteoclastogenesis of haematopoietic stem cells. In summary, mature adipocytes suppress osteogenesis by enhancing the formation of osteoclasts, which eventually lead to low bone mass. Further studies are required to find out the novel factor and its detailed underlying mechanism in promoting osteoclastogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/288239
ISSN
2023 Impact Factor: 3.1
2023 SCImago Journal Rankings: 0.261

 

DC FieldValueLanguage
dc.contributor.authorCheong, LY-
dc.contributor.authorLiu, Z-
dc.contributor.authorWang, B-
dc.contributor.authorMa, Y-
dc.contributor.authorXu, A-
dc.date.accessioned2020-10-05T12:09:57Z-
dc.date.available2020-10-05T12:09:57Z-
dc.date.issued2020-
dc.identifier.citationThe 25th Annual Medical Research Conference, Hong Kong, 18 January 2020. In Hong Kong Medical Journal, 2020, v. 26 n. 1, Suppl. 1, p. 28, abstract no. 43-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/288239-
dc.description.abstractIntroduction: Substantial clinical evidence demonstrating a complex relationship between adiposity and osteoporosis suggests that adipose tissue plays an important role in bone metabolism. However, the exact function of adipocytes and mechanisms underlying its impact on bone health are yet incompletely understood due to marked discrepancies in human and animal studies. In this study, the effect of adipocytes on osteogenesis was explored using a novel lipodystrophic mouse model. Methods: We generated adipose tissue-specific murine double minute 2 knockout (KO) mice with Cre/loxP system. Knockout mice displayed gradually loss of peripheral adipose tissue and bone marrow adipocytes until nearly 80% to 100% of total fat reduction at 12-week-old mice. Results: Micro-computed tomography revealed that bone mineralisation density of femur from 12-week-old KO mice was significantly higher than the wild-type (WT) mice. Interestingly, the absence of adipocytes in KO mice exhibited markedly lower serum levels of C-terminal telopeptides of type I collagen (bone resorption marker) than WT mice, whereas the N-terminal propeptides of type I collagen (bone formation marker) remained unaffected. No differences were observed in the ex vivo differentiation of osteoblasts and osteoclasts between WT and KO mice. In addition, adipocyte-conditioned media treatment of isolated haematopoietic stem cells from bone marrow enhanced the differentiation of osteoclasts. Conclusion: These results indicate that secreted factors from adipocytes may promote osteoclastogenesis of haematopoietic stem cells. In summary, mature adipocytes suppress osteogenesis by enhancing the formation of osteoclasts, which eventually lead to low bone mass. Further studies are required to find out the novel factor and its detailed underlying mechanism in promoting osteoclastogenesis.-
dc.languageeng-
dc.publisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org/-
dc.relation.ispartofHong Kong Medical Journal-
dc.relation.ispartof25th Medical Research Conference-
dc.rightsHong Kong Medical Journal. Copyright © Hong Kong Academy of Medicine Press.-
dc.titleAdipocytes regulate osteogenesis in a novel lipoatrophic mouse model-
dc.typeConference_Paper-
dc.identifier.emailCheong, LY: u3003285@connect.hku.hk-
dc.identifier.emailWang, B: baile612@hku.hk-
dc.identifier.emailXu, A: amxu@hkucc.hku.hk-
dc.identifier.authorityXu, A=rp00485-
dc.identifier.hkuros315464-
dc.identifier.volume26-
dc.identifier.issue1, Suppl. 1-
dc.identifier.spage28, abstract no. 43-
dc.identifier.epage28, abstract no. 43-
dc.publisher.placeHong Kong-
dc.identifier.issnl1024-2708-

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