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Article: Prevent action of magnoflorine with hyaluronic acid gel from cartilage degeneration in anterior cruciate ligament transection induced osteoarthritis

TitlePrevent action of magnoflorine with hyaluronic acid gel from cartilage degeneration in anterior cruciate ligament transection induced osteoarthritis
Authors
KeywordsMagnoflorine
Osteoarthritis
Articular cartilage
Subchondral trabecular bone
Hyaluronic acid gel
Issue Date2020
PublisherElsevier France, Editions Scientifiques et Medicales. The Journal's web site is located at http://www.elsevier.com/locate/biopha
Citation
Biomedicine & Pharmacotherapy, 2020, v. 126, p. article no. 109733 How to Cite?
AbstractAccording to the Chinese medicine, magnoflorine exerted significant anti-inflammatory effects and potentially promoted synthesis of proteoglycans in chondrocytes to reverse the progression of rheumatoid arthritis. However, the latent beneficial effect of magnoflorine for the treatment of traumatic osteoarthritis (OA) is still unknown. Therefore, we aim to demonstrate the efficacy of magnoflorine combined with HA-gel in attenuating cartilage degeneration in anterior cruciate ligament transection (ACLT) induced OA rat model. We found that the histological results showed the elevated cartilage matrix, chondrogenic signals and chondroprogenitor cells in HA-gel + magnoflorine treatment. HA-gel + magnoflorine treatment resulted in a decreased modified Mankin’s score, and a higher volume ratio of hyaline cartilage (HC)/calcified cartilage (CC) and HC/Sum (whole cartilage), compared to ACLT and HA-gel groups. Furthermore, both the volume ratios of HC/Sum and HC/CC were negatively correlated with modified Mankin’s scores. Finally, HA-gel + magnoflorine could significantly increase the BV/TV, Tb.Th, and decrease the Tb.Pf, Po(tot), Conn.Dn and Tb.Sp. In vitro, 50 μg/ml magnoflorine treatment could significantly increase the viability, S-phase, migration rate and chondrogenesis of chondroprogenitor cells. There were significant downregulations of MAPK/NF-κB signaling, and upregulations of chondrogenic signals in 50 μg/ml magnoflorine treatment. There were significant downregulations of proinflammatory cytokines and upregulation of IL-10 in HA-gel + magnoflorine treated group. Therefore, our study elucidated a protective effect of HA-gel + magnoflorine on attenuating cartilage degradation and maintaining SCB stabilization in ACLT induced OA.
Persistent Identifierhttp://hdl.handle.net/10722/288123
ISSN
2019 Impact Factor: 4.545
2015 SCImago Journal Rankings: 0.787

 

DC FieldValueLanguage
dc.contributor.authorCai, Z-
dc.contributor.authorHong, M-
dc.contributor.authorXU, L-
dc.contributor.authorYang, K-
dc.contributor.authorLI, C-
dc.contributor.authorSUN, T-
dc.contributor.authorFeng, Y-
dc.contributor.authorZeng, H-
dc.contributor.authorLu, WW-
dc.contributor.authorChiu, KY-
dc.date.accessioned2020-10-05T12:08:12Z-
dc.date.available2020-10-05T12:08:12Z-
dc.date.issued2020-
dc.identifier.citationBiomedicine & Pharmacotherapy, 2020, v. 126, p. article no. 109733-
dc.identifier.issn0753-3322-
dc.identifier.urihttp://hdl.handle.net/10722/288123-
dc.description.abstractAccording to the Chinese medicine, magnoflorine exerted significant anti-inflammatory effects and potentially promoted synthesis of proteoglycans in chondrocytes to reverse the progression of rheumatoid arthritis. However, the latent beneficial effect of magnoflorine for the treatment of traumatic osteoarthritis (OA) is still unknown. Therefore, we aim to demonstrate the efficacy of magnoflorine combined with HA-gel in attenuating cartilage degeneration in anterior cruciate ligament transection (ACLT) induced OA rat model. We found that the histological results showed the elevated cartilage matrix, chondrogenic signals and chondroprogenitor cells in HA-gel + magnoflorine treatment. HA-gel + magnoflorine treatment resulted in a decreased modified Mankin’s score, and a higher volume ratio of hyaline cartilage (HC)/calcified cartilage (CC) and HC/Sum (whole cartilage), compared to ACLT and HA-gel groups. Furthermore, both the volume ratios of HC/Sum and HC/CC were negatively correlated with modified Mankin’s scores. Finally, HA-gel + magnoflorine could significantly increase the BV/TV, Tb.Th, and decrease the Tb.Pf, Po(tot), Conn.Dn and Tb.Sp. In vitro, 50 μg/ml magnoflorine treatment could significantly increase the viability, S-phase, migration rate and chondrogenesis of chondroprogenitor cells. There were significant downregulations of MAPK/NF-κB signaling, and upregulations of chondrogenic signals in 50 μg/ml magnoflorine treatment. There were significant downregulations of proinflammatory cytokines and upregulation of IL-10 in HA-gel + magnoflorine treated group. Therefore, our study elucidated a protective effect of HA-gel + magnoflorine on attenuating cartilage degradation and maintaining SCB stabilization in ACLT induced OA.-
dc.languageeng-
dc.publisherElsevier France, Editions Scientifiques et Medicales. The Journal's web site is located at http://www.elsevier.com/locate/biopha-
dc.relation.ispartofBiomedicine & Pharmacotherapy-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectMagnoflorine-
dc.subjectOsteoarthritis-
dc.subjectArticular cartilage-
dc.subjectSubchondral trabecular bone-
dc.subjectHyaluronic acid gel-
dc.titlePrevent action of magnoflorine with hyaluronic acid gel from cartilage degeneration in anterior cruciate ligament transection induced osteoarthritis-
dc.typeArticle-
dc.identifier.emailLu, WW: wwlu@hku.hk-
dc.identifier.emailChiu, KY: pkychiu@hkucc.hku.hk-
dc.identifier.authorityLu, WW=rp00411-
dc.identifier.authorityChiu, KY=rp00379-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.biopha.2019.109733-
dc.identifier.pmid32113051-
dc.identifier.scopuseid_2-s2.0-85079860048-
dc.identifier.hkuros315184-
dc.identifier.volume126-
dc.identifier.spagearticle no. 109733-
dc.identifier.epagearticle no. 109733-
dc.publisher.placeFrance-

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