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Article: Establishment of porcine and human expanded potential stem cells

TitleEstablishment of porcine and human expanded potential stem cells
Authors
Keywordsbiotechnology
chimera
embryo
gene editing
gene expression
Issue Date2019
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/naturecellbiology
Citation
Nature Cell Biology, 2019, v. 21 n. 6, p. 687-699 How to Cite?
AbstractWe recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation. EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all embryonic and extra-embryonic cell lineages. Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro. Under similar conditions, human embryonic stem cells and induced pluripotent stem cells can be converted, or somatic cells directly reprogrammed, to EPSCs that display the molecular and functional attributes reminiscent of porcine EPSCs. Importantly, trophoblast stem-cell-like cells can be generated from both human and porcine EPSCs. Our pathway-inhibition paradigm thus opens an avenue for generating mammalian pluripotent stem cells, and EPSCs present a unique cellular platform for translational research in biotechnology and regenerative medicine.
Persistent Identifierhttp://hdl.handle.net/10722/287592
ISSN
2023 Impact Factor: 17.3
2023 SCImago Journal Rankings: 8.913
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGao, X-
dc.contributor.authorNowak-Imialek, M-
dc.contributor.authorChen, X-
dc.contributor.authorChen, D-
dc.contributor.authorHerrmann, D-
dc.contributor.authorRuan, D-
dc.contributor.authorChen, ACH-
dc.contributor.authorEckersley-Maslin, MA-
dc.contributor.authorAhmad, S-
dc.contributor.authorLee, YL-
dc.contributor.authorKobayashi, T-
dc.contributor.authorRyan, D-
dc.contributor.authorZhong, J-
dc.contributor.authorZhu, J-
dc.contributor.authorWu, J-
dc.contributor.authorLan, G-
dc.contributor.authorPetkov, S-
dc.contributor.authorYang, J-
dc.contributor.authorAntunes, L-
dc.contributor.authorCampos, LS-
dc.contributor.authorFu, B-
dc.contributor.authorWang, S-
dc.contributor.authorYong, Y-
dc.contributor.authorWang, X-
dc.contributor.authorXue, SG-
dc.contributor.authorGe, L-
dc.contributor.authorLiu, Z-
dc.contributor.authorHuang, Y-
dc.contributor.authorNie, T-
dc.contributor.authorLi, P-
dc.contributor.authorWu, D-
dc.contributor.authorPei, D-
dc.contributor.authorZhang, Y-
dc.contributor.authorLu, L-
dc.contributor.authorYang, F-
dc.contributor.authorKimber, SJ-
dc.contributor.authorReik, W-
dc.contributor.authorZou, X-
dc.contributor.authorShang, Z-
dc.contributor.authorLai, L-
dc.contributor.authorSurani, A-
dc.contributor.authorTam, PPL-
dc.contributor.authorAhmed, A-
dc.contributor.authorYeung, WSB-
dc.contributor.authorTeichmann, SA-
dc.contributor.authorNiemann, H-
dc.contributor.authorLiu, P-
dc.date.accessioned2020-10-05T12:00:20Z-
dc.date.available2020-10-05T12:00:20Z-
dc.date.issued2019-
dc.identifier.citationNature Cell Biology, 2019, v. 21 n. 6, p. 687-699-
dc.identifier.issn1465-7392-
dc.identifier.urihttp://hdl.handle.net/10722/287592-
dc.description.abstractWe recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation. EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all embryonic and extra-embryonic cell lineages. Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro. Under similar conditions, human embryonic stem cells and induced pluripotent stem cells can be converted, or somatic cells directly reprogrammed, to EPSCs that display the molecular and functional attributes reminiscent of porcine EPSCs. Importantly, trophoblast stem-cell-like cells can be generated from both human and porcine EPSCs. Our pathway-inhibition paradigm thus opens an avenue for generating mammalian pluripotent stem cells, and EPSCs present a unique cellular platform for translational research in biotechnology and regenerative medicine.-
dc.languageeng-
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/naturecellbiology-
dc.relation.ispartofNature Cell Biology-
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: https://doi.org/[insert DOI]-
dc.subjectbiotechnology-
dc.subjectchimera-
dc.subjectembryo-
dc.subjectgene editing-
dc.subjectgene expression-
dc.titleEstablishment of porcine and human expanded potential stem cells-
dc.typeArticle-
dc.identifier.emailRuan, D: degong77@hku.hk-
dc.identifier.emailWu, J: jackng83@hku.hk-
dc.identifier.emailLiu, P: pliu88@hku.hk-
dc.identifier.authorityLiu, P=rp02328-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1038/s41556-019-0333-2-
dc.identifier.pmid31160711-
dc.identifier.pmcidPMC7035105-
dc.identifier.scopuseid_2-s2.0-85066957043-
dc.identifier.hkuros315739-
dc.identifier.volume21-
dc.identifier.issue6-
dc.identifier.spage687-
dc.identifier.epage699-
dc.identifier.isiWOS:000470080100006-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1465-7392-

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