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Article: Preemptive Homology-Directed DNA Repair Fosters Complex Genomic Rearrangements in Hepatocellular Carcinoma

TitlePreemptive Homology-Directed DNA Repair Fosters Complex Genomic Rearrangements in Hepatocellular Carcinoma
Authors
Keywordsaneuploidy
antineoplastic activity
apoptosis
autophosphorylation
carcinogenesis
Issue Date2020
PublisherElsevier: Creative Commons Attribution Non-Commercial No-Derivatives License. The Journal's web site is located at http://www.transonc.com
Citation
Translational Oncology, 2020, v. 13 n. 9, p. article no. 100796 How to Cite?
AbstractDegree of genomic instability closely correlates with poor prognosis, drug resistance as well as poor survival across human cancer of different origins. This study assessed the relationship between DNA damage response (DDR) and chromosome instability in hepatocellular carcinoma (HCC). We investigated DDR signaling in HCC cells by analyzing DNA damage-dependent redistribution of major DDR proteins to damaged chromatin using immunofluorescence microscopy and Western blotting experimentations. We also performed gene conversion and metaphase analyses to address whether dysregulated DDR may bear any biological significance during hepatocarcinogenesis. Accordingly, we found that HCC cell lines suffered from elevated spontaneous DNA double-strand breaks (DSBs). In addition, analyses of HCC metaphases revealed marked aneuploidy and frequent sister chromatid exchanges when compared to immortalized hepatocytes, the latter of which were further induced following camptothecin-induced DSBs. We propose that genomic instability in HCC may be caused by erroneous DNA repair in a desperate attempt to mend DSBs for cell survival and that such preemptive measures inadvertently foster chromosome instability and thus complex genomic rearrangements.
Persistent Identifierhttp://hdl.handle.net/10722/287587
ISSN
2015 Impact Factor: 3.077
2023 SCImago Journal Rankings: 1.263
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSy, SMH-
dc.contributor.authorGUO, Y-
dc.contributor.authorLan, YING-
dc.contributor.authorNg, H-
dc.contributor.authorHuen, MSY-
dc.date.accessioned2020-10-05T12:00:15Z-
dc.date.available2020-10-05T12:00:15Z-
dc.date.issued2020-
dc.identifier.citationTranslational Oncology, 2020, v. 13 n. 9, p. article no. 100796-
dc.identifier.issn1944-7124-
dc.identifier.urihttp://hdl.handle.net/10722/287587-
dc.description.abstractDegree of genomic instability closely correlates with poor prognosis, drug resistance as well as poor survival across human cancer of different origins. This study assessed the relationship between DNA damage response (DDR) and chromosome instability in hepatocellular carcinoma (HCC). We investigated DDR signaling in HCC cells by analyzing DNA damage-dependent redistribution of major DDR proteins to damaged chromatin using immunofluorescence microscopy and Western blotting experimentations. We also performed gene conversion and metaphase analyses to address whether dysregulated DDR may bear any biological significance during hepatocarcinogenesis. Accordingly, we found that HCC cell lines suffered from elevated spontaneous DNA double-strand breaks (DSBs). In addition, analyses of HCC metaphases revealed marked aneuploidy and frequent sister chromatid exchanges when compared to immortalized hepatocytes, the latter of which were further induced following camptothecin-induced DSBs. We propose that genomic instability in HCC may be caused by erroneous DNA repair in a desperate attempt to mend DSBs for cell survival and that such preemptive measures inadvertently foster chromosome instability and thus complex genomic rearrangements.-
dc.languageeng-
dc.publisherElsevier: Creative Commons Attribution Non-Commercial No-Derivatives License. The Journal's web site is located at http://www.transonc.com-
dc.relation.ispartofTranslational Oncology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectaneuploidy-
dc.subjectantineoplastic activity-
dc.subjectapoptosis-
dc.subjectautophosphorylation-
dc.subjectcarcinogenesis-
dc.titlePreemptive Homology-Directed DNA Repair Fosters Complex Genomic Rearrangements in Hepatocellular Carcinoma-
dc.typeArticle-
dc.identifier.emailSy, SMH: mhsy@hku.hk-
dc.identifier.emailHuen, MSY: huen.michael@hku.hk-
dc.identifier.authorityHuen, MSY=rp01336-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.tranon.2020.100796-
dc.identifier.pmid32450552-
dc.identifier.pmcidPMC7256322-
dc.identifier.scopuseid_2-s2.0-85085042777-
dc.identifier.hkuros315487-
dc.identifier.volume13-
dc.identifier.issue9-
dc.identifier.spagearticle no. 100796-
dc.identifier.epagearticle no. 100796-
dc.identifier.isiWOS:000552466200003-
dc.publisher.placeUnited States-
dc.identifier.issnl1936-5233-

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