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Article: Costunolide Plays an Anti-Neuroinflammation Role in Lipopolysaccharide-Induced BV2 Microglial Activation by Targeting Cyclin-Dependent Kinase 2
Title | Costunolide Plays an Anti-Neuroinflammation Role in Lipopolysaccharide-Induced BV2 Microglial Activation by Targeting Cyclin-Dependent Kinase 2 |
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Authors | |
Keywords | costunolide natural product anti-neuroinflammation target identification CDK2 |
Issue Date | 2020 |
Publisher | Molecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/molecules |
Citation | Molecules, 2020, v. 25 n. 12, p. article no. 2840 How to Cite? |
Abstract | Hyperactivation of microglia in the brain is closely related to neuroinflammation and leads to neuronal dysfunction. Costunolide (CTL) is a natural sesquiterpene lactone with wide pharmacological activities including anti-inflammation and antioxidation. In this study, we found that CTL significantly inhibited the production of inflammatory mediators including nitric oxide, IL-6, TNF-α, and PGE2 in lipopolysaccharide (LPS)-stimulated BV2 microglia. Moreover, CTL effectively attenuated IKKβ/NF-κB signaling pathway activation. To identify direct cellular target of CTL, we performed high-throughput reverse virtual screening assay using scPDB protein structure library, and found cyclin-dependent kinase 2 (CDK2) was the most specific binding protein for CTL. We further confirmed the binding ability of CTL with CDK2 using cellular thermal shift assay (CETSA) and drug affinity responsive target stability (DARTS) assays. Surface plasmon resonance analysis also supported that CTL specifically bound to CDK2 with a dissociation constant at micromole level. Furthermore, knocking down CDK2 obviously reversed the anti-inflammation effect of CTL via AKT/IKKβ/NF-κB signaling pathway on BV-2 cells. Collectively, these results indicate that CTL inhibits microglia-mediated neuroinflammation through directly targeting CDK2, and provide insights into the role of CDK2 as a promising anti-neuroinflammation therapeutic target. |
Description | eid_2-s2.0-85086966578 |
Persistent Identifier | http://hdl.handle.net/10722/287212 |
ISSN | 2023 Impact Factor: 4.2 2023 SCImago Journal Rankings: 0.744 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | LIU, YC | - |
dc.contributor.author | Feng, N | - |
dc.contributor.author | Li, WW | - |
dc.contributor.author | Tu, PF | - |
dc.contributor.author | Chen, JP | - |
dc.contributor.author | Han, JY | - |
dc.contributor.author | zeng, KW | - |
dc.date.accessioned | 2020-09-22T02:57:32Z | - |
dc.date.available | 2020-09-22T02:57:32Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Molecules, 2020, v. 25 n. 12, p. article no. 2840 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | http://hdl.handle.net/10722/287212 | - |
dc.description | eid_2-s2.0-85086966578 | - |
dc.description.abstract | Hyperactivation of microglia in the brain is closely related to neuroinflammation and leads to neuronal dysfunction. Costunolide (CTL) is a natural sesquiterpene lactone with wide pharmacological activities including anti-inflammation and antioxidation. In this study, we found that CTL significantly inhibited the production of inflammatory mediators including nitric oxide, IL-6, TNF-α, and PGE2 in lipopolysaccharide (LPS)-stimulated BV2 microglia. Moreover, CTL effectively attenuated IKKβ/NF-κB signaling pathway activation. To identify direct cellular target of CTL, we performed high-throughput reverse virtual screening assay using scPDB protein structure library, and found cyclin-dependent kinase 2 (CDK2) was the most specific binding protein for CTL. We further confirmed the binding ability of CTL with CDK2 using cellular thermal shift assay (CETSA) and drug affinity responsive target stability (DARTS) assays. Surface plasmon resonance analysis also supported that CTL specifically bound to CDK2 with a dissociation constant at micromole level. Furthermore, knocking down CDK2 obviously reversed the anti-inflammation effect of CTL via AKT/IKKβ/NF-κB signaling pathway on BV-2 cells. Collectively, these results indicate that CTL inhibits microglia-mediated neuroinflammation through directly targeting CDK2, and provide insights into the role of CDK2 as a promising anti-neuroinflammation therapeutic target. | - |
dc.language | eng | - |
dc.publisher | Molecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/molecules | - |
dc.relation.ispartof | Molecules | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | costunolide | - |
dc.subject | natural product | - |
dc.subject | anti-neuroinflammation | - |
dc.subject | target identification | - |
dc.subject | CDK2 | - |
dc.title | Costunolide Plays an Anti-Neuroinflammation Role in Lipopolysaccharide-Induced BV2 Microglial Activation by Targeting Cyclin-Dependent Kinase 2 | - |
dc.type | Article | - |
dc.identifier.email | Chen, JP: abchen@hkucc.hku.hk | - |
dc.identifier.authority | Chen, JP=rp01316 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3390/molecules25122840 | - |
dc.identifier.pmid | 32575562 | - |
dc.identifier.pmcid | PMC7355650 | - |
dc.identifier.scopus | eid_2-s2.0-85086966578 | - |
dc.identifier.hkuros | 314502 | - |
dc.identifier.volume | 25 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | article no. 2840 | - |
dc.identifier.epage | article no. 2840 | - |
dc.identifier.isi | WOS:000550243800001 | - |
dc.publisher.place | Switzerland | - |
dc.identifier.issnl | 1420-3049 | - |