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Article: Establishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment

TitleEstablishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment
Authors
KeywordsHeart failure
Intramyocardial injection
Issue 159
Medicine
Myocardial infarction
Issue Date2020
PublisherJournal of Visualized Experiments. The Journal's web site is located at http://www.jove.com
Citation
Journal of Visualized Experiments, 2020, v. 159, p. article no. e60392 How to Cite?
AbstractAlthough advances have been achieved in the treatment of heart failure (HF) following myocardial infarction (MI), HF following MI remains one of the major causes of mortality and morbidity around the world. Cell-based therapies for cardiac repair and improvement of left ventricular function after MI have attracted considerable attention. Accordingly, the safety and efficacy of these cell transplantations should be tested in a preclinical large animal model of HF prior to clinical use. Pigs are widely used for cardiovascular disease research due to their similarity to humans in terms of heart size and coronary anatomy. Therefore, we sought to present an effective protocol for the establishment of a porcine chronic HF model using closed-chest coronary balloon occlusion of the left circumflex artery (LCX), followed by rapid ventricular pacing induced with pacemaker implantation. Eight weeks later, the stem cells were administered by intramyocardial injection in the peri-infarct area. Then the infarct size, cell survival, and left ventricular function (including echocardiography, hemodynamic parameters, and electrophysiology) were evaluated. This study helps establish a stable preclinical large animal HF model for stem cell treatment.
Persistent Identifierhttp://hdl.handle.net/10722/287156
ISSN
2023 Impact Factor: 1.2
2023 SCImago Journal Rankings: 0.449
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSUN, S-
dc.contributor.authorJIANG, Y-
dc.contributor.authorZhen, Z-
dc.contributor.authorLai, WH-
dc.contributor.authorLiao, S-
dc.contributor.authorTse, HF-
dc.date.accessioned2020-09-22T02:56:38Z-
dc.date.available2020-09-22T02:56:38Z-
dc.date.issued2020-
dc.identifier.citationJournal of Visualized Experiments, 2020, v. 159, p. article no. e60392-
dc.identifier.issn1940-087X-
dc.identifier.urihttp://hdl.handle.net/10722/287156-
dc.description.abstractAlthough advances have been achieved in the treatment of heart failure (HF) following myocardial infarction (MI), HF following MI remains one of the major causes of mortality and morbidity around the world. Cell-based therapies for cardiac repair and improvement of left ventricular function after MI have attracted considerable attention. Accordingly, the safety and efficacy of these cell transplantations should be tested in a preclinical large animal model of HF prior to clinical use. Pigs are widely used for cardiovascular disease research due to their similarity to humans in terms of heart size and coronary anatomy. Therefore, we sought to present an effective protocol for the establishment of a porcine chronic HF model using closed-chest coronary balloon occlusion of the left circumflex artery (LCX), followed by rapid ventricular pacing induced with pacemaker implantation. Eight weeks later, the stem cells were administered by intramyocardial injection in the peri-infarct area. Then the infarct size, cell survival, and left ventricular function (including echocardiography, hemodynamic parameters, and electrophysiology) were evaluated. This study helps establish a stable preclinical large animal HF model for stem cell treatment.-
dc.languageeng-
dc.publisherJournal of Visualized Experiments. The Journal's web site is located at http://www.jove.com-
dc.relation.ispartofJournal of Visualized Experiments-
dc.rightsJournal of Visualized Experiments. Copyright © Journal of Visualized Experiments.-
dc.subjectHeart failure-
dc.subjectIntramyocardial injection-
dc.subjectIssue 159-
dc.subjectMedicine-
dc.subjectMyocardial infarction-
dc.titleEstablishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment-
dc.typeArticle-
dc.identifier.emailZhen, Z: zhenzhe@HKUCC-COM.hku.hk-
dc.identifier.emailLai, WH: kwhlai@hku.hk-
dc.identifier.emailLiao, S: lsy923@hku.hk-
dc.identifier.emailTse, HF: hftse@hkucc.hku.hk-
dc.identifier.authorityLiao, S=rp02244-
dc.identifier.authorityTse, HF=rp00428-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3791/60392-
dc.identifier.pmid32510509-
dc.identifier.scopuseid_2-s2.0-85086135303-
dc.identifier.hkuros314397-
dc.identifier.volume159-
dc.identifier.issue159-
dc.identifier.spagearticle no. e60392-
dc.identifier.epagearticle no. e60392-
dc.identifier.isiWOS:000546406600148-
dc.publisher.placeUnited States-
dc.identifier.issnl1940-087X-

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