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Article: ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection

TitleORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection
Authors
Hachim, AKavian, NCohen, CAChin, AWHChu, DKWMok, CKPTsang, OTYYeung, YCPerera, RAPMPoon, LLMPeiris, JSMValkenburg, SA
Keywordsadult
aged
Betacoronavirus
blood
Coronavirus infection
Issue Date2020
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ni
Citation
Nature Immunology, 2020, v. 21, p. 1293-1301 How to Cite?
DOI: http://dx.doi.org/10.1038/s41590-020-0773-7
AbstractThe SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity.
Persistent Identifierhttp://hdl.handle.net/10722/287120
ISSN
1529-2908
2023 Impact Factor: 27.7
2023 SCImago Journal Rankings: 11.274
ISI Accession Number ID
WOS:000561014100001

 

DC FieldValueLanguage
dc.contributor.authorHachim, A-
dc.contributor.authorKavian, N-
dc.contributor.authorCohen, CA-
dc.contributor.authorChin, AWH-
dc.contributor.authorChu, DKW-
dc.contributor.authorMok, CKP-
dc.contributor.authorTsang, OTY-
dc.contributor.authorYeung, YC-
dc.contributor.authorPerera, RAPM-
dc.contributor.authorPoon, LLM-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorValkenburg, SA-
dc.date.accessioned2020-09-22T02:56:03Z-
dc.date.available2020-09-22T02:56:03Z-
dc.date.issued2020-
dc.identifier.citationNature Immunology, 2020, v. 21, p. 1293-1301-
dc.identifier.issn1529-2908-
dc.identifier.urihttp://hdl.handle.net/10722/287120-
dc.description.abstractThe SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity.-
dc.languageeng-
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ni-
dc.relation.ispartofNature Immunology-
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: https://doi.org/[insert DOI]-
dc.subjectadult-
dc.subjectaged-
dc.subjectBetacoronavirus-
dc.subjectblood-
dc.subjectCoronavirus infection-
dc.titleORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection-
dc.typeArticle-
dc.identifier.emailHachim, A: ahachim@hku.hk-
dc.identifier.emailKavian, N: niloufar@hku.hk-
dc.identifier.emailChin, AWH: alexchin@hku.hk-
dc.identifier.emailChu, DKW: dkwchu@hku.hk-
dc.identifier.emailMok, CKP: ch02mkp@hkucc.hku.hk-
dc.identifier.emailPerera, RAPM: mahenp@hku.hk-
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.emailValkenburg, SA: sophiev@hku.hk-
dc.identifier.authorityChin, AWH=rp02345-
dc.identifier.authorityChu, DKW=rp02512-
dc.identifier.authorityMok, CKP=rp01805-
dc.identifier.authorityPerera, RAPM=rp02500-
dc.identifier.authorityPoon, LLM=rp00484-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.identifier.authorityValkenburg, SA=rp02141-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/s41590-020-0773-7-
dc.identifier.pmid32807944-
dc.identifier.scopuseid_2-s2.0-85089555434-
dc.identifier.hkuros314271-
dc.identifier.volume21-
dc.identifier.spage1293-
dc.identifier.epage1301-
dc.identifier.isiWOS:000561014100001-
dc.publisher.placeUnited States-
dc.identifier.issnl1529-2908-

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