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Article: ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection

TitleORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection
Authors
Keywordsadult
aged
Betacoronavirus
blood
Coronavirus infection
Issue Date2020
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ni
Citation
Nature Immunology, 2020, v. 21, p. 1293-1301 How to Cite?
AbstractThe SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity.
Persistent Identifierhttp://hdl.handle.net/10722/287120
ISSN
2020 Impact Factor: 25.606
2020 SCImago Journal Rankings: 9.074
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHachim, A-
dc.contributor.authorKavian, N-
dc.contributor.authorCohen, CA-
dc.contributor.authorChin, AWH-
dc.contributor.authorChu, DKW-
dc.contributor.authorMok, CKP-
dc.contributor.authorTsang, OTY-
dc.contributor.authorYeung, YC-
dc.contributor.authorPerera, RAPM-
dc.contributor.authorPoon, LLM-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorValkenburg, SA-
dc.date.accessioned2020-09-22T02:56:03Z-
dc.date.available2020-09-22T02:56:03Z-
dc.date.issued2020-
dc.identifier.citationNature Immunology, 2020, v. 21, p. 1293-1301-
dc.identifier.issn1529-2908-
dc.identifier.urihttp://hdl.handle.net/10722/287120-
dc.description.abstractThe SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity.-
dc.languageeng-
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ni-
dc.relation.ispartofNature Immunology-
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: https://doi.org/[insert DOI]-
dc.subjectadult-
dc.subjectaged-
dc.subjectBetacoronavirus-
dc.subjectblood-
dc.subjectCoronavirus infection-
dc.titleORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection-
dc.typeArticle-
dc.identifier.emailHachim, A: ahachim@hku.hk-
dc.identifier.emailKavian, N: niloufar@hku.hk-
dc.identifier.emailChin, AWH: alexchin@hku.hk-
dc.identifier.emailChu, DKW: dkwchu@hku.hk-
dc.identifier.emailMok, CKP: ch02mkp@hkucc.hku.hk-
dc.identifier.emailPerera, RAPM: mahenp@hku.hk-
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.emailValkenburg, SA: sophiev@hku.hk-
dc.identifier.authorityChin, AWH=rp02345-
dc.identifier.authorityChu, DKW=rp02512-
dc.identifier.authorityMok, CKP=rp01805-
dc.identifier.authorityPerera, RAPM=rp02500-
dc.identifier.authorityPoon, LLM=rp00484-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.identifier.authorityValkenburg, SA=rp02141-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/s41590-020-0773-7-
dc.identifier.pmid32807944-
dc.identifier.scopuseid_2-s2.0-85089555434-
dc.identifier.hkuros314271-
dc.identifier.volume21-
dc.identifier.spage1293-
dc.identifier.epage1301-
dc.identifier.isiWOS:000561014100001-
dc.publisher.placeUnited States-
dc.identifier.issnl1529-2908-

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