File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Identification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68

TitleIdentification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68
Authors
Issue Date2004
Citation
Journal of Virology, 2004, v. 78, n. 17, p. 9123-9131 How to Cite?
AbstractHuman gammaherpesviruses are associated with lymphomas and other malignancies. Murine gammaherpesvirus 68 (MHV-68) infection of mice has emerged as a model for understanding gammaherpesvirus pathogenesis in vivo. In contrast to human gammaherpesviruses, MHV-68 replicates in permissive cell lines in a robust manner, presenting an efficient model to study the basic mechanisms for DNA replication and recombination processes. In addition, MHV-68 also infects a broad range of cells of different tissue types and from different host species, and the viral genome persists as an episome in infected cells. These features make MHV-68 an attractive system on which to build gene delivery vectors. We have therefore undertaken a study to identify the cis elements required for MHV-68 genome replication and packaging. Here we report that an 8.4-kb MHV-68 genomic fragment between ORF66 and ORF73 conferred on the plasmid the ability to replicate; replication required the presence of either de novo viral infection or viral reactivation from latency. We further mapped the origin of lytic replication (oriLyt) to a 1.25-kb region. Moreover, we demonstrated that the terminal repeat of the viral genome is sufficient for packaging of the replicated oriLyt plasmid into mature viral particles. Functional identification of the MHV-68 oriLyt and packaging signal has laid a foundation for investigating the mechanisms controlling gammaherpesvirus DNA replication during the viral lytic phase and will also serve as a base on which to design gene delivery vectors.
Persistent Identifierhttp://hdl.handle.net/10722/285919
ISSN
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.378
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDeng, Hongyu-
dc.contributor.authorChu, Julia T.-
dc.contributor.authorPark, No Hee-
dc.contributor.authorSun, Ren-
dc.date.accessioned2020-08-18T04:56:59Z-
dc.date.available2020-08-18T04:56:59Z-
dc.date.issued2004-
dc.identifier.citationJournal of Virology, 2004, v. 78, n. 17, p. 9123-9131-
dc.identifier.issn0022-538X-
dc.identifier.urihttp://hdl.handle.net/10722/285919-
dc.description.abstractHuman gammaherpesviruses are associated with lymphomas and other malignancies. Murine gammaherpesvirus 68 (MHV-68) infection of mice has emerged as a model for understanding gammaherpesvirus pathogenesis in vivo. In contrast to human gammaherpesviruses, MHV-68 replicates in permissive cell lines in a robust manner, presenting an efficient model to study the basic mechanisms for DNA replication and recombination processes. In addition, MHV-68 also infects a broad range of cells of different tissue types and from different host species, and the viral genome persists as an episome in infected cells. These features make MHV-68 an attractive system on which to build gene delivery vectors. We have therefore undertaken a study to identify the cis elements required for MHV-68 genome replication and packaging. Here we report that an 8.4-kb MHV-68 genomic fragment between ORF66 and ORF73 conferred on the plasmid the ability to replicate; replication required the presence of either de novo viral infection or viral reactivation from latency. We further mapped the origin of lytic replication (oriLyt) to a 1.25-kb region. Moreover, we demonstrated that the terminal repeat of the viral genome is sufficient for packaging of the replicated oriLyt plasmid into mature viral particles. Functional identification of the MHV-68 oriLyt and packaging signal has laid a foundation for investigating the mechanisms controlling gammaherpesvirus DNA replication during the viral lytic phase and will also serve as a base on which to design gene delivery vectors.-
dc.languageeng-
dc.relation.ispartofJournal of Virology-
dc.titleIdentification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JVI.78.17.9123-9131.2004-
dc.identifier.pmid15308708-
dc.identifier.pmcidPMC506910-
dc.identifier.scopuseid_2-s2.0-4143097024-
dc.identifier.volume78-
dc.identifier.issue17-
dc.identifier.spage9123-
dc.identifier.epage9131-
dc.identifier.isiWOS:000223386600017-
dc.identifier.issnl0022-538X-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats