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Article: Genome-wide identification of interferon-sensitive mutations enables influenza vaccine design

TitleGenome-wide identification of interferon-sensitive mutations enables influenza vaccine design
Authors
Issue Date2018
Citation
Science, 2018, v. 359, n. 6373, p. 290-296 How to Cite?
AbstractIn conventional attenuated viral vaccines, immunogenicity is often suboptimal. Here we present a systematic approach for vaccine development that eliminates interferon (IFN)-modulating functions genome-wide while maintaining virus replication fitness.We applied a quantitative high-Throughput genomics system to influenza A virus that simultaneously measured the replication fitness and IFN sensitivity of mutations across the entire genome. By incorporating eight IFN-sensitive mutations, we generated a hyper-interferon-sensitive (HIS) virus as a vaccine candidate. HIS virus is highly attenuated in IFN-competent hosts but able to induce transient IFN responses, elicits robust humoral and cellular immune responses, and provides protection against homologous and heterologous viral challenges. Our approach, which attenuates the virus and promotes immune responses concurrently, is broadly applicable for vaccine development against other pathogens.
Persistent Identifierhttp://hdl.handle.net/10722/285801
ISSN
2020 Impact Factor: 47.728
2015 SCImago Journal Rankings: 13.217
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDu, Yushen-
dc.contributor.authorXin, Li-
dc.contributor.authorShi, Yuan-
dc.contributor.authorZhang, Tian Hao-
dc.contributor.authorWu, Nicholas C.-
dc.contributor.authorDai, Lei-
dc.contributor.authorGong, Danyang-
dc.contributor.authorBrar, Gurpreet-
dc.contributor.authorShu, Sara-
dc.contributor.authorLuo, Jiadi-
dc.contributor.authorReiley, William-
dc.contributor.authorTseng, Yen Wen-
dc.contributor.authorBai, Hongyan-
dc.contributor.authorWu, Ting Ting-
dc.contributor.authorWang, Jieru-
dc.contributor.authorShu, Yuelong-
dc.contributor.authorSun, Ren-
dc.date.accessioned2020-08-18T04:56:41Z-
dc.date.available2020-08-18T04:56:41Z-
dc.date.issued2018-
dc.identifier.citationScience, 2018, v. 359, n. 6373, p. 290-296-
dc.identifier.issn0036-8075-
dc.identifier.urihttp://hdl.handle.net/10722/285801-
dc.description.abstractIn conventional attenuated viral vaccines, immunogenicity is often suboptimal. Here we present a systematic approach for vaccine development that eliminates interferon (IFN)-modulating functions genome-wide while maintaining virus replication fitness.We applied a quantitative high-Throughput genomics system to influenza A virus that simultaneously measured the replication fitness and IFN sensitivity of mutations across the entire genome. By incorporating eight IFN-sensitive mutations, we generated a hyper-interferon-sensitive (HIS) virus as a vaccine candidate. HIS virus is highly attenuated in IFN-competent hosts but able to induce transient IFN responses, elicits robust humoral and cellular immune responses, and provides protection against homologous and heterologous viral challenges. Our approach, which attenuates the virus and promotes immune responses concurrently, is broadly applicable for vaccine development against other pathogens.-
dc.languageeng-
dc.relation.ispartofScience-
dc.titleGenome-wide identification of interferon-sensitive mutations enables influenza vaccine design-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1126/science.aan8806-
dc.identifier.pmid29348231-
dc.identifier.scopuseid_2-s2.0-85040570901-
dc.identifier.volume359-
dc.identifier.issue6373-
dc.identifier.spage290-
dc.identifier.epage296-
dc.identifier.eissn1095-9203-
dc.identifier.isiWOS:000423236700031-
dc.identifier.issnl0036-8075-

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