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Article: Intrinsic disorder mediates hepatitis C virus core-host cell protein interactions

TitleIntrinsic disorder mediates hepatitis C virus core-host cell protein interactions
Authors
Keywordshepatitis virus
protein-protein interactions
systems biology
viral protein
virus
hepatitis C virus
intrinsically disordered proteins
host-pathogen interactions
Issue Date2015
Citation
Protein Science, 2015, v. 24, n. 2, p. 221-235 How to Cite?
Abstract© 2014 The Protein Society. Viral proteins bind to numerous cellular and viral proteins throughout the infection cycle. However, the mechanisms by which viral proteins interact with such large numbers of factors remain unknown. Cellular proteins that interact with multiple, distinct partners often do so through short sequences known as molecular recognition features (MoRFs) embedded within intrinsically disordered regions (IDRs). In this study, we report the first evidence that MoRFs in viral proteins play a similar role in targeting the host cell. Using a combination of evolutionary modeling, protein-protein interaction analyses and forward genetic screening, we systematically investigated two computationally predicted MoRFs within the N-terminal IDR of the hepatitis C virus (HCV) Core protein. Sequence analysis of the MoRFs showed their conservation across all HCV genotypes and the canine and equine Hepaciviruses. Phylogenetic modeling indicated that the Core MoRFs are under stronger purifying selection than the surrounding sequence, suggesting that these modules have a biological function. Using the yeast two-hybrid assay, we identified three cellular binding partners for each HCV Core MoRF, including two previously characterized cellular targets of HCV Core (DDX3X and NPM1). Random and site-directed mutagenesis demonstrated that the predicted MoRF regions were required for binding to the cellular proteins, but that different residues within each MoRF were critical for binding to different partners. This study demonstrated that viruses may use intrinsic disorder to target multiple cellular proteins with the same amino acid sequence and provides a framework for characterizing the binding partners of other disordered regions in viral and cellular proteomes.
Persistent Identifierhttp://hdl.handle.net/10722/285764
ISSN
2023 Impact Factor: 4.5
2023 SCImago Journal Rankings: 4.419
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDolan, Patrick T.-
dc.contributor.authorRoth, Andrew P.-
dc.contributor.authorXue, Bin-
dc.contributor.authorSun, Ren-
dc.contributor.authorDunker, A. Keith-
dc.contributor.authorUversky, Vladimir N.-
dc.contributor.authorLacount, Douglas J.-
dc.date.accessioned2020-08-18T04:56:35Z-
dc.date.available2020-08-18T04:56:35Z-
dc.date.issued2015-
dc.identifier.citationProtein Science, 2015, v. 24, n. 2, p. 221-235-
dc.identifier.issn0961-8368-
dc.identifier.urihttp://hdl.handle.net/10722/285764-
dc.description.abstract© 2014 The Protein Society. Viral proteins bind to numerous cellular and viral proteins throughout the infection cycle. However, the mechanisms by which viral proteins interact with such large numbers of factors remain unknown. Cellular proteins that interact with multiple, distinct partners often do so through short sequences known as molecular recognition features (MoRFs) embedded within intrinsically disordered regions (IDRs). In this study, we report the first evidence that MoRFs in viral proteins play a similar role in targeting the host cell. Using a combination of evolutionary modeling, protein-protein interaction analyses and forward genetic screening, we systematically investigated two computationally predicted MoRFs within the N-terminal IDR of the hepatitis C virus (HCV) Core protein. Sequence analysis of the MoRFs showed their conservation across all HCV genotypes and the canine and equine Hepaciviruses. Phylogenetic modeling indicated that the Core MoRFs are under stronger purifying selection than the surrounding sequence, suggesting that these modules have a biological function. Using the yeast two-hybrid assay, we identified three cellular binding partners for each HCV Core MoRF, including two previously characterized cellular targets of HCV Core (DDX3X and NPM1). Random and site-directed mutagenesis demonstrated that the predicted MoRF regions were required for binding to the cellular proteins, but that different residues within each MoRF were critical for binding to different partners. This study demonstrated that viruses may use intrinsic disorder to target multiple cellular proteins with the same amino acid sequence and provides a framework for characterizing the binding partners of other disordered regions in viral and cellular proteomes.-
dc.languageeng-
dc.relation.ispartofProtein Science-
dc.subjecthepatitis virus-
dc.subjectprotein-protein interactions-
dc.subjectsystems biology-
dc.subjectviral protein-
dc.subjectvirus-
dc.subjecthepatitis C virus-
dc.subjectintrinsically disordered proteins-
dc.subjecthost-pathogen interactions-
dc.titleIntrinsic disorder mediates hepatitis C virus core-host cell protein interactions-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/pro.2608-
dc.identifier.pmid25424537-
dc.identifier.pmcidPMC4315660-
dc.identifier.scopuseid_2-s2.0-84961288711-
dc.identifier.volume24-
dc.identifier.issue2-
dc.identifier.spage221-
dc.identifier.epage235-
dc.identifier.eissn1469-896X-
dc.identifier.isiWOS:000348663200007-
dc.identifier.issnl0961-8368-

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