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Article: Unconventional sequence requirement for viral late gene core promoters of murine gammaherpesvirus 68

TitleUnconventional sequence requirement for viral late gene core promoters of murine gammaherpesvirus 68
Authors
Issue Date2014
Citation
Journal of Virology, 2014, v. 88, n. 6, p. 3411-3422 How to Cite?
AbstractInfection with the human gammaherpesviruses, Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV), is associated with several cancers. During lytic replication of herpesviruses, viral genes are expressed in an ordered cascade. However, the mechanism by which late gene expression is regulated has not been well characterized in gammaherpesviruses. In this study, we have investigated the cis element that mediates late gene expression during de novo lytic infection with murine gammaherpesvirus 68 (MHV-68). A reporter system was established and used to assess the activity of viral late gene promoters upon infection with MHV-68. It was found that the viral origin of lytic replication, orilyt, must be on the reporter plasmid to support activation of the late gene promoter. Furthermore, the DNA sequence required for the activation of late gene promoters was mapped to a core element containing a distinct TATT box and its neighboring sequences. The critical nucleotides of the TATT box region were determined by systematic mutagenesis in the reporter system, and the significance of these nucleotides was confirmed in the context of the viral genome. In addition, EBV and KSHV late gene core promoters could be activated by MHV-68 lytic replication, indicating that the mechanisms controlling late gene expression are conserved among gammaherpesviruses. Therefore, our results on MHV-68 establish a solid foundation for mechanistic studies of late gene regulation. © 2014, American Society for Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/285724
ISSN
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.378
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong-Ho, Elaine-
dc.contributor.authorWu, Ting Ting-
dc.contributor.authorDavis, Zoe H.-
dc.contributor.authorZhang, Bingqing-
dc.contributor.authorHuang, Jian-
dc.contributor.authorGong, Hao-
dc.contributor.authorDeng, Hongyu-
dc.contributor.authorLiu, Fenyong-
dc.contributor.authorGlaunsinger, Britt-
dc.contributor.authorSun, Ren-
dc.date.accessioned2020-08-18T04:56:28Z-
dc.date.available2020-08-18T04:56:28Z-
dc.date.issued2014-
dc.identifier.citationJournal of Virology, 2014, v. 88, n. 6, p. 3411-3422-
dc.identifier.issn0022-538X-
dc.identifier.urihttp://hdl.handle.net/10722/285724-
dc.description.abstractInfection with the human gammaherpesviruses, Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV), is associated with several cancers. During lytic replication of herpesviruses, viral genes are expressed in an ordered cascade. However, the mechanism by which late gene expression is regulated has not been well characterized in gammaherpesviruses. In this study, we have investigated the cis element that mediates late gene expression during de novo lytic infection with murine gammaherpesvirus 68 (MHV-68). A reporter system was established and used to assess the activity of viral late gene promoters upon infection with MHV-68. It was found that the viral origin of lytic replication, orilyt, must be on the reporter plasmid to support activation of the late gene promoter. Furthermore, the DNA sequence required for the activation of late gene promoters was mapped to a core element containing a distinct TATT box and its neighboring sequences. The critical nucleotides of the TATT box region were determined by systematic mutagenesis in the reporter system, and the significance of these nucleotides was confirmed in the context of the viral genome. In addition, EBV and KSHV late gene core promoters could be activated by MHV-68 lytic replication, indicating that the mechanisms controlling late gene expression are conserved among gammaherpesviruses. Therefore, our results on MHV-68 establish a solid foundation for mechanistic studies of late gene regulation. © 2014, American Society for Microbiology.-
dc.languageeng-
dc.relation.ispartofJournal of Virology-
dc.titleUnconventional sequence requirement for viral late gene core promoters of murine gammaherpesvirus 68-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JVI.01374-13-
dc.identifier.pmid24403583-
dc.identifier.pmcidPMC3957950-
dc.identifier.scopuseid_2-s2.0-84894535908-
dc.identifier.volume88-
dc.identifier.issue6-
dc.identifier.spage3411-
dc.identifier.epage3422-
dc.identifier.eissn1098-5514-
dc.identifier.isiWOS:000332126000032-
dc.identifier.issnl0022-538X-

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