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Article: The virion-associated open reading frame 49 of murine gammaherpesvirus 68 promotes viral replication both In Vitro and In Vivo as a derepressor of RTA

TitleThe virion-associated open reading frame 49 of murine gammaherpesvirus 68 promotes viral replication both In Vitro and In Vivo as a derepressor of RTA
Authors
Issue Date2012
Citation
Journal of Virology, 2012, v. 86, n. 2, p. 1109-1118 How to Cite?
AbstractReplication and transcription activator (RTA), an immediate-early gene, is a key molecular switch to evoke lytic replication of gammaherpesviruses. Open reading frame 49 (ORF49) is conserved among gammaherpesviruses and shown to cooperate with RTA in regulating virus lytic replication. Here we show a molecular mechanism and in vivo functions of murine gammaherpesvirus 68 (MHV-68 or γHV-68) ORF49. MHV-68 ORF49 was transcribed and translated as a late gene. The ORF49 protein was associated with a virion, interacting with the ORF64 large tegument protein and the ORF25 capsid protein. Moreover, ORF49 directly bound to RTA and its negative cellular regulator, poly(ADP-ribose) polymerase-1 (PARP-1), and disrupted the interactions of RTA and PARP-1. Productive replication of an ORF49-deficient mutant virus (49S) was attenuated in vivo as well as in vitro. Likewise, latent infection was also impaired in the spleen of 49S-infected mice. Taken together, our results suggest that the virion-associated ORF49 protein may promote virus replication both in vitro and in vivo by providing an optimal environment in the early phase of virus infection as a derepressor of RTA. © 2012, American Society for Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/285696
ISSN
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.378
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNoh, Cheol Woo-
dc.contributor.authorCho, Hye Jeong-
dc.contributor.authorKang, Hye Ri-
dc.contributor.authorJin, Hyun Yong-
dc.contributor.authorLee, Shaoying-
dc.contributor.authorDeng, Hongyu-
dc.contributor.authorWu, Ting Ting-
dc.contributor.authorArumugaswami, Vaithilingaraja-
dc.contributor.authorSun, Ren-
dc.contributor.authorSong, Moon Jung-
dc.date.accessioned2020-08-18T04:56:24Z-
dc.date.available2020-08-18T04:56:24Z-
dc.date.issued2012-
dc.identifier.citationJournal of Virology, 2012, v. 86, n. 2, p. 1109-1118-
dc.identifier.issn0022-538X-
dc.identifier.urihttp://hdl.handle.net/10722/285696-
dc.description.abstractReplication and transcription activator (RTA), an immediate-early gene, is a key molecular switch to evoke lytic replication of gammaherpesviruses. Open reading frame 49 (ORF49) is conserved among gammaherpesviruses and shown to cooperate with RTA in regulating virus lytic replication. Here we show a molecular mechanism and in vivo functions of murine gammaherpesvirus 68 (MHV-68 or γHV-68) ORF49. MHV-68 ORF49 was transcribed and translated as a late gene. The ORF49 protein was associated with a virion, interacting with the ORF64 large tegument protein and the ORF25 capsid protein. Moreover, ORF49 directly bound to RTA and its negative cellular regulator, poly(ADP-ribose) polymerase-1 (PARP-1), and disrupted the interactions of RTA and PARP-1. Productive replication of an ORF49-deficient mutant virus (49S) was attenuated in vivo as well as in vitro. Likewise, latent infection was also impaired in the spleen of 49S-infected mice. Taken together, our results suggest that the virion-associated ORF49 protein may promote virus replication both in vitro and in vivo by providing an optimal environment in the early phase of virus infection as a derepressor of RTA. © 2012, American Society for Microbiology.-
dc.languageeng-
dc.relation.ispartofJournal of Virology-
dc.titleThe virion-associated open reading frame 49 of murine gammaherpesvirus 68 promotes viral replication both In Vitro and In Vivo as a derepressor of RTA-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JVI.05785-11-
dc.identifier.pmid22090108-
dc.identifier.pmcidPMC3255801-
dc.identifier.scopuseid_2-s2.0-84863127136-
dc.identifier.volume86-
dc.identifier.issue2-
dc.identifier.spage1109-
dc.identifier.epage1118-
dc.identifier.eissn1098-5514-
dc.identifier.isiWOS:000298674600042-
dc.identifier.issnl0022-538X-

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