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Article: Rapid mRNA-display selection of an IL-6 inhibitor using continuous-flow magnetic separation

TitleRapid mRNA-display selection of an IL-6 inhibitor using continuous-flow magnetic separation
Authors
Olson, C. AndersAdams, Jonathan D.Takahashi, Terry T.Qi, HangfeiHowell, Shannon M.Wu, Ting TingRoberts, Richard W.Sun, RenSoh, H. Tom
KeywordsmRNA
selection methods
ligand design
directed evolution
antibodies
Issue Date2011
Citation
Angewandte Chemie - International Edition, 2011, v. 50, n. 36, p. 8295-8298 How to Cite?
DOI: http://dx.doi.org/10.1002/anie.201101149
AbstractEven flow: Microfluidics technology can rapidly generate affinity reagents that bind to protein targets using mRNA display. The continuous-flow magnetic separation (CFMS) method (see scheme; steps a-h) offers reproducible means to impose stringent selection conditions without the need for a specialized device. The method yields approximately a 30-fold greater partition efficiency over conventional methods when using interleukin-6 as a model target. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/285683
ISSN
1433-7851
2022 Impact Factor: 16.6
2020 SCImago Journal Rankings: 5.831
PubMed Central ID
PMC3747967
ISI Accession Number ID
WOS:000295259700013

 

DC FieldValueLanguage
dc.contributor.authorOlson, C. Anders-
dc.contributor.authorAdams, Jonathan D.-
dc.contributor.authorTakahashi, Terry T.-
dc.contributor.authorQi, Hangfei-
dc.contributor.authorHowell, Shannon M.-
dc.contributor.authorWu, Ting Ting-
dc.contributor.authorRoberts, Richard W.-
dc.contributor.authorSun, Ren-
dc.contributor.authorSoh, H. Tom-
dc.date.accessioned2020-08-18T04:56:22Z-
dc.date.available2020-08-18T04:56:22Z-
dc.date.issued2011-
dc.identifier.citationAngewandte Chemie - International Edition, 2011, v. 50, n. 36, p. 8295-8298-
dc.identifier.issn1433-7851-
dc.identifier.urihttp://hdl.handle.net/10722/285683-
dc.description.abstractEven flow: Microfluidics technology can rapidly generate affinity reagents that bind to protein targets using mRNA display. The continuous-flow magnetic separation (CFMS) method (see scheme; steps a-h) offers reproducible means to impose stringent selection conditions without the need for a specialized device. The method yields approximately a 30-fold greater partition efficiency over conventional methods when using interleukin-6 as a model target. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.-
dc.languageeng-
dc.relation.ispartofAngewandte Chemie - International Edition-
dc.subjectmRNA-
dc.subjectselection methods-
dc.subjectligand design-
dc.subjectdirected evolution-
dc.subjectantibodies-
dc.titleRapid mRNA-display selection of an IL-6 inhibitor using continuous-flow magnetic separation-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/anie.201101149-
dc.identifier.pmid21761516-
dc.identifier.pmcidPMC3747967-
dc.identifier.scopuseid_2-s2.0-80052079213-
dc.identifier.volume50-
dc.identifier.issue36-
dc.identifier.spage8295-
dc.identifier.epage8298-
dc.identifier.eissn1521-3773-
dc.identifier.isiWOS:000295259700013-
dc.identifier.f100013361052-
dc.identifier.issnl1433-7851-

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