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Article: Genetic analysis of H1N1 influenza virus from throat swab samples in a microfluidic system for point-of-care diagnostics

TitleGenetic analysis of H1N1 influenza virus from throat swab samples in a microfluidic system for point-of-care diagnostics
Authors
Issue Date2011
Citation
Journal of the American Chemical Society, 2011, v. 133, n. 23, p. 9129-9135 How to Cite?
AbstractThe ability to obtain sequence-specific genetic information about rare target organisms directly from complex biological samples at the point-of-care would transform many areas of biotechnology. Microfluidics technology offers compelling tools for integrating multiple biochemical processes in a single device, but despite significant progress, only limited examples have shown specific, genetic analysis of clinical samples within the context of a fully integrated, portable platform. Herein we present the Magnetic Integrated Microfluidic Electrochemical Detector (MIMED) that integrates sample preparation and electrochemical sensors in a monolithic disposable device to detect RNA-based virus directly from throat swab samples. By combining immunomagnetic target capture, concentration, and purification, reverse-transcriptase polymerase chain reaction (RT-PCR) and single-stranded DNA (ssDNA) generation in the sample preparation chamber, as well as sequence-specific electrochemical DNA detection in the electrochemical cell, we demonstrate the detection of influenza H1N1 in throat swab samples at loads as low as 10 TCID50, 4 orders of magnitude below the clinical titer for this virus. Given the availability of affinity reagents for a broad range of pathogens, our system offers a general approach for multitarget diagnostics at the point-of-care. © 2011 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/285680
ISSN
2023 Impact Factor: 14.4
2023 SCImago Journal Rankings: 5.489
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFerguson, B. Scott-
dc.contributor.authorBuchsbaum, Steven F.-
dc.contributor.authorWu, Ting Ting-
dc.contributor.authorHsieh, Kuangwen-
dc.contributor.authorXiao, Yi-
dc.contributor.authorSun, Ren-
dc.contributor.authorSoh, H. Tom-
dc.date.accessioned2020-08-18T04:56:22Z-
dc.date.available2020-08-18T04:56:22Z-
dc.date.issued2011-
dc.identifier.citationJournal of the American Chemical Society, 2011, v. 133, n. 23, p. 9129-9135-
dc.identifier.issn0002-7863-
dc.identifier.urihttp://hdl.handle.net/10722/285680-
dc.description.abstractThe ability to obtain sequence-specific genetic information about rare target organisms directly from complex biological samples at the point-of-care would transform many areas of biotechnology. Microfluidics technology offers compelling tools for integrating multiple biochemical processes in a single device, but despite significant progress, only limited examples have shown specific, genetic analysis of clinical samples within the context of a fully integrated, portable platform. Herein we present the Magnetic Integrated Microfluidic Electrochemical Detector (MIMED) that integrates sample preparation and electrochemical sensors in a monolithic disposable device to detect RNA-based virus directly from throat swab samples. By combining immunomagnetic target capture, concentration, and purification, reverse-transcriptase polymerase chain reaction (RT-PCR) and single-stranded DNA (ssDNA) generation in the sample preparation chamber, as well as sequence-specific electrochemical DNA detection in the electrochemical cell, we demonstrate the detection of influenza H1N1 in throat swab samples at loads as low as 10 TCID50, 4 orders of magnitude below the clinical titer for this virus. Given the availability of affinity reagents for a broad range of pathogens, our system offers a general approach for multitarget diagnostics at the point-of-care. © 2011 American Chemical Society.-
dc.languageeng-
dc.relation.ispartofJournal of the American Chemical Society-
dc.titleGenetic analysis of H1N1 influenza virus from throat swab samples in a microfluidic system for point-of-care diagnostics-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1021/ja203981w-
dc.identifier.pmid21561070-
dc.identifier.pmcidPMC3110979-
dc.identifier.scopuseid_2-s2.0-79958785745-
dc.identifier.volume133-
dc.identifier.issue23-
dc.identifier.spage9129-
dc.identifier.epage9135-
dc.identifier.eissn1520-5126-
dc.identifier.isiWOS:000291667600058-
dc.identifier.issnl0002-7863-

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