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- Publisher Website: 10.1006/viro.1995.1269
- Scopus: eid_2-s2.0-0029007411
- PMID: 7778272
- WOS: WOS:A1995RC35100011
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Article: Transmissible Retrovirus in Epstein-Burr Virus-Producer B95-8 Cells
Title | Transmissible Retrovirus in Epstein-Burr Virus-Producer B95-8 Cells |
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Authors | |
Issue Date | 1995 |
Citation | Virology, 1995, v. 209, n. 2, p. 374-383 How to Cite? |
Abstract | Epstein-Burr virus (EBV) released from the B95-8 marmoset cell line has served as a prototype for biologic and biochemical studies of EBV. Here we identify and characterize a retrovirus carried by many cultures of B95-8 cells. The experiments were stimulated by the isolation of a cDNA clone from B95-8 cells in which sequences from the EBV large internal repeat were linked to gag sequences similar to those of squirrel monkey retrovirus, human isolate, SMRV-H. However, among 413 amino acids predicted from the nucleotide sequence of the gag region of the B95-8 SMRV isolate there were 48 amino acid changes that distinguished this virus from SMRV-H originally isolated from a human lymphoid cell line by Oda et al. (1988, Virology 167, 468-476). Nucleic acid and antibody probes were developed for the B95-8 isolate of SMRV. Using such probes, we found that SMRV-B95-8 was readily transmissible, independent of EBV, as an infectious virus to human and T cell lines, SMRV-B95-8 was highly fusogenic in the presence or absence of EBV. The ultrastructural appearance of the B95-8 retrovirus was characteristic of a type D retrovirus. Cells dually infected with EBV and SMRV-B95-8 did not demonstrate increased levels of lyric EB vital replication. SMRV-B95-8 did not by itself cause lymphocyte immortalization or enhance immortalization by EBV. Thus SMRV-B95-8 does not contribute to the major biologic properties of the B95-8 strain of EBV. © 1995 Academic Press. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/285551 |
ISSN | 2021 Impact Factor: 3.513 2020 SCImago Journal Rankings: 1.389 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Sun, Ren | - |
dc.contributor.author | Grogan, Elizabeth | - |
dc.contributor.author | Shedd, Duane | - |
dc.contributor.author | Bykovsky, Albert F. | - |
dc.contributor.author | Kushnaryov, Vladimir M. | - |
dc.contributor.author | Grossberg, Sidney E. | - |
dc.contributor.author | Miller, George | - |
dc.date.accessioned | 2020-08-18T04:56:02Z | - |
dc.date.available | 2020-08-18T04:56:02Z | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | Virology, 1995, v. 209, n. 2, p. 374-383 | - |
dc.identifier.issn | 0042-6822 | - |
dc.identifier.uri | http://hdl.handle.net/10722/285551 | - |
dc.description.abstract | Epstein-Burr virus (EBV) released from the B95-8 marmoset cell line has served as a prototype for biologic and biochemical studies of EBV. Here we identify and characterize a retrovirus carried by many cultures of B95-8 cells. The experiments were stimulated by the isolation of a cDNA clone from B95-8 cells in which sequences from the EBV large internal repeat were linked to gag sequences similar to those of squirrel monkey retrovirus, human isolate, SMRV-H. However, among 413 amino acids predicted from the nucleotide sequence of the gag region of the B95-8 SMRV isolate there were 48 amino acid changes that distinguished this virus from SMRV-H originally isolated from a human lymphoid cell line by Oda et al. (1988, Virology 167, 468-476). Nucleic acid and antibody probes were developed for the B95-8 isolate of SMRV. Using such probes, we found that SMRV-B95-8 was readily transmissible, independent of EBV, as an infectious virus to human and T cell lines, SMRV-B95-8 was highly fusogenic in the presence or absence of EBV. The ultrastructural appearance of the B95-8 retrovirus was characteristic of a type D retrovirus. Cells dually infected with EBV and SMRV-B95-8 did not demonstrate increased levels of lyric EB vital replication. SMRV-B95-8 did not by itself cause lymphocyte immortalization or enhance immortalization by EBV. Thus SMRV-B95-8 does not contribute to the major biologic properties of the B95-8 strain of EBV. © 1995 Academic Press. All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Virology | - |
dc.title | Transmissible Retrovirus in Epstein-Burr Virus-Producer B95-8 Cells | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1006/viro.1995.1269 | - |
dc.identifier.pmid | 7778272 | - |
dc.identifier.scopus | eid_2-s2.0-0029007411 | - |
dc.identifier.volume | 209 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 374 | - |
dc.identifier.epage | 383 | - |
dc.identifier.eissn | 1096-0341 | - |
dc.identifier.isi | WOS:A1995RC35100011 | - |
dc.identifier.issnl | 0042-6822 | - |