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Article: Protamine-Induced Supramolecular Self-Assembly of Red-Emissive Alkynylplatinum(II) 2,6-Bis(benzimidazol-2′-yl)pyridine Complex for Selective Label-Free Sensing of Heparin and Real-Time Monitoring of Trypsin Activity

TitleProtamine-Induced Supramolecular Self-Assembly of Red-Emissive Alkynylplatinum(II) 2,6-Bis(benzimidazol-2′-yl)pyridine Complex for Selective Label-Free Sensing of Heparin and Real-Time Monitoring of Trypsin Activity
Authors
Keywordstrypsin assay
biosensors
protamine
heparin
platinum
Issue Date2019
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/aamick
Citation
ACS Applied Materials & Interfaces, 2019, v. 11 n. 35, p. 31585-31593 How to Cite?
AbstractA label-free detection assay is developed based on the design and synthesis of a new anionic alkynylplatinum(II) 2,6-bis(benzimidazol-2′-yl)pyridine complex with water-soluble pendants. With the aid of electrostatic interaction and noncovalent metal–metal and π–π stacking interactions, protamine is shown to induce supramolecular self-assembly of platinum(II) complexes with drastic UV–vis absorption and red emission changes. On the basis of the strong binding affinity of protamine and heparin, the ensemble has been further employed to probe heparin by monitoring the spectroscopic changes. Other than heparin, this ensemble can also detect the activity of trypsin, which can hydrolyze protamine into fragments, leading to the deaggregation of platinum(II) complexes. By modulation of the self-assembly properties of platinum(II) complexes via real-time UV–vis absorption and emission studies, the reported assay has been demonstrated to be a sensitive and selective detection method for trypsin, as well as trypsin inhibitor screening, which is essential for drug discovery.
Persistent Identifierhttp://hdl.handle.net/10722/285422
ISSN
2020 Impact Factor: 9.229
2015 SCImago Journal Rankings: 2.381
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCHAN, CWT-
dc.contributor.authorCHENG, HK-
dc.contributor.authorHau, FKW-
dc.contributor.authorChan, AKW-
dc.contributor.authorYam, VWW-
dc.date.accessioned2020-08-18T03:53:17Z-
dc.date.available2020-08-18T03:53:17Z-
dc.date.issued2019-
dc.identifier.citationACS Applied Materials & Interfaces, 2019, v. 11 n. 35, p. 31585-31593-
dc.identifier.issn1944-8244-
dc.identifier.urihttp://hdl.handle.net/10722/285422-
dc.description.abstractA label-free detection assay is developed based on the design and synthesis of a new anionic alkynylplatinum(II) 2,6-bis(benzimidazol-2′-yl)pyridine complex with water-soluble pendants. With the aid of electrostatic interaction and noncovalent metal–metal and π–π stacking interactions, protamine is shown to induce supramolecular self-assembly of platinum(II) complexes with drastic UV–vis absorption and red emission changes. On the basis of the strong binding affinity of protamine and heparin, the ensemble has been further employed to probe heparin by monitoring the spectroscopic changes. Other than heparin, this ensemble can also detect the activity of trypsin, which can hydrolyze protamine into fragments, leading to the deaggregation of platinum(II) complexes. By modulation of the self-assembly properties of platinum(II) complexes via real-time UV–vis absorption and emission studies, the reported assay has been demonstrated to be a sensitive and selective detection method for trypsin, as well as trypsin inhibitor screening, which is essential for drug discovery.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/aamick-
dc.relation.ispartofACS Applied Materials & Interfaces-
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].-
dc.subjecttrypsin assay-
dc.subjectbiosensors-
dc.subjectprotamine-
dc.subjectheparin-
dc.subjectplatinum-
dc.titleProtamine-Induced Supramolecular Self-Assembly of Red-Emissive Alkynylplatinum(II) 2,6-Bis(benzimidazol-2′-yl)pyridine Complex for Selective Label-Free Sensing of Heparin and Real-Time Monitoring of Trypsin Activity-
dc.typeArticle-
dc.identifier.emailYam, VWW: wwyam@hku.hk-
dc.identifier.authorityYam, VWW=rp00822-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acsami.9b08653-
dc.identifier.pmid31436404-
dc.identifier.scopuseid_2-s2.0-85071788796-
dc.identifier.hkuros313038-
dc.identifier.volume11-
dc.identifier.issue35-
dc.identifier.spage31585-
dc.identifier.epage31593-
dc.identifier.isiWOS:000484831100002-
dc.publisher.placeUnited States-
dc.identifier.issnl1944-8244-

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