File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.3390/pharmaceutics12080732
- Scopus: eid_2-s2.0-85090523652
- WOS: WOS:000564127900001
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: In Vitro Release Study of the Polymeric Drug Nanoparticles: Development and Validation of a Novel Method
Title | In Vitro Release Study of the Polymeric Drug Nanoparticles: Development and Validation of a Novel Method |
---|---|
Authors | |
Keywords | polymeric nanoparticle in vitro release kinetics centrifugal ultrafiltration dialysis filtration |
Issue Date | 2020 |
Publisher | MDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/pharmaceuticals/ |
Citation | Pharmaceutics, 2020, v. 12 n. 8, p. article no. 732 How to Cite? |
Abstract | The in vitro release study is a critical test to assess the safety, efficacy, and quality of nanoparticle-based drug delivery systems, but there is no compendial or regulatory standard. The variety of testing methods makes direct comparison among different systems difficult. We herein proposed a novel sample and separate (SS) method by combining the United States Pharmacopeia (USP) apparatus II (paddle) with well-validated centrifugal ultrafiltration (CU) technique that efficiently separated the free drug from nanoparticles. Polymeric drug nanoparticles were prepared by using a four-stream multi-inlet vortex mixer with d-α-tocopheryl polyethylene glycol 1000 succinate as a stabilizer. Itraconazole, cholecalciferol, and flurbiprofen were selected to produce three different nanoparticles with particle size <100 nm. By comparing with the dialysis membrane (DM) method and the SS methods using syringe filters, this novel SS + CU technique was considered the most appropriate in terms of the accuracy and repeatability to provide the in vitro release kinetics of nanoparticles. Interestingly, the DM method appeared to misestimate the release kinetics of nanoparticles through separate mechanisms. This work offers a superior analytical technique for studying in vitro drug release from polymeric nanoparticles, which could benefit the future development of in vitro-in vivo correlation of polymeric nanoparticles. |
Persistent Identifier | http://hdl.handle.net/10722/284944 |
ISSN | 2023 Impact Factor: 4.3 2023 SCImago Journal Rankings: 0.845 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | WENG, J | - |
dc.contributor.author | Tong, HHY | - |
dc.contributor.author | Chow, SF | - |
dc.date.accessioned | 2020-08-07T09:04:40Z | - |
dc.date.available | 2020-08-07T09:04:40Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Pharmaceutics, 2020, v. 12 n. 8, p. article no. 732 | - |
dc.identifier.issn | 1424-8247 | - |
dc.identifier.uri | http://hdl.handle.net/10722/284944 | - |
dc.description.abstract | The in vitro release study is a critical test to assess the safety, efficacy, and quality of nanoparticle-based drug delivery systems, but there is no compendial or regulatory standard. The variety of testing methods makes direct comparison among different systems difficult. We herein proposed a novel sample and separate (SS) method by combining the United States Pharmacopeia (USP) apparatus II (paddle) with well-validated centrifugal ultrafiltration (CU) technique that efficiently separated the free drug from nanoparticles. Polymeric drug nanoparticles were prepared by using a four-stream multi-inlet vortex mixer with d-α-tocopheryl polyethylene glycol 1000 succinate as a stabilizer. Itraconazole, cholecalciferol, and flurbiprofen were selected to produce three different nanoparticles with particle size <100 nm. By comparing with the dialysis membrane (DM) method and the SS methods using syringe filters, this novel SS + CU technique was considered the most appropriate in terms of the accuracy and repeatability to provide the in vitro release kinetics of nanoparticles. Interestingly, the DM method appeared to misestimate the release kinetics of nanoparticles through separate mechanisms. This work offers a superior analytical technique for studying in vitro drug release from polymeric nanoparticles, which could benefit the future development of in vitro-in vivo correlation of polymeric nanoparticles. | - |
dc.language | eng | - |
dc.publisher | MDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/pharmaceuticals/ | - |
dc.relation.ispartof | Pharmaceutics | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | polymeric nanoparticle | - |
dc.subject | in vitro release kinetics | - |
dc.subject | centrifugal ultrafiltration | - |
dc.subject | dialysis | - |
dc.subject | filtration | - |
dc.title | In Vitro Release Study of the Polymeric Drug Nanoparticles: Development and Validation of a Novel Method | - |
dc.type | Article | - |
dc.identifier.email | Tong, HHY: henry002@hku.hk | - |
dc.identifier.email | Chow, SF: asfchow@hku.hk | - |
dc.identifier.authority | Chow, SF=rp02296 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3390/pharmaceutics12080732 | - |
dc.identifier.scopus | eid_2-s2.0-85090523652 | - |
dc.identifier.hkuros | 312595 | - |
dc.identifier.volume | 12 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | article no. 732 | - |
dc.identifier.epage | article no. 732 | - |
dc.identifier.isi | WOS:000564127900001 | - |
dc.publisher.place | Switzerland | - |
dc.identifier.issnl | 1424-8247 | - |