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Article: In Vitro Release Study of the Polymeric Drug Nanoparticles: Development and Validation of a Novel Method

TitleIn Vitro Release Study of the Polymeric Drug Nanoparticles: Development and Validation of a Novel Method
Authors
Keywordspolymeric nanoparticle
in vitro release kinetics
centrifugal ultrafiltration
dialysis
filtration
Issue Date2020
PublisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/pharmaceuticals/
Citation
Pharmaceutics, 2020, v. 12 n. 8, p. article no. 732 How to Cite?
AbstractThe in vitro release study is a critical test to assess the safety, efficacy, and quality of nanoparticle-based drug delivery systems, but there is no compendial or regulatory standard. The variety of testing methods makes direct comparison among different systems difficult. We herein proposed a novel sample and separate (SS) method by combining the United States Pharmacopeia (USP) apparatus II (paddle) with well-validated centrifugal ultrafiltration (CU) technique that efficiently separated the free drug from nanoparticles. Polymeric drug nanoparticles were prepared by using a four-stream multi-inlet vortex mixer with d-α-tocopheryl polyethylene glycol 1000 succinate as a stabilizer. Itraconazole, cholecalciferol, and flurbiprofen were selected to produce three different nanoparticles with particle size <100 nm. By comparing with the dialysis membrane (DM) method and the SS methods using syringe filters, this novel SS + CU technique was considered the most appropriate in terms of the accuracy and repeatability to provide the in vitro release kinetics of nanoparticles. Interestingly, the DM method appeared to misestimate the release kinetics of nanoparticles through separate mechanisms. This work offers a superior analytical technique for studying in vitro drug release from polymeric nanoparticles, which could benefit the future development of in vitro-in vivo correlation of polymeric nanoparticles.
Persistent Identifierhttp://hdl.handle.net/10722/284944
ISSN
2015 SCImago Journal Rankings: 1.245

 

DC FieldValueLanguage
dc.contributor.authorWENG, J-
dc.contributor.authorTong, HHY-
dc.contributor.authorChow, SF-
dc.date.accessioned2020-08-07T09:04:40Z-
dc.date.available2020-08-07T09:04:40Z-
dc.date.issued2020-
dc.identifier.citationPharmaceutics, 2020, v. 12 n. 8, p. article no. 732-
dc.identifier.issn1424-8247-
dc.identifier.urihttp://hdl.handle.net/10722/284944-
dc.description.abstractThe in vitro release study is a critical test to assess the safety, efficacy, and quality of nanoparticle-based drug delivery systems, but there is no compendial or regulatory standard. The variety of testing methods makes direct comparison among different systems difficult. We herein proposed a novel sample and separate (SS) method by combining the United States Pharmacopeia (USP) apparatus II (paddle) with well-validated centrifugal ultrafiltration (CU) technique that efficiently separated the free drug from nanoparticles. Polymeric drug nanoparticles were prepared by using a four-stream multi-inlet vortex mixer with d-α-tocopheryl polyethylene glycol 1000 succinate as a stabilizer. Itraconazole, cholecalciferol, and flurbiprofen were selected to produce three different nanoparticles with particle size <100 nm. By comparing with the dialysis membrane (DM) method and the SS methods using syringe filters, this novel SS + CU technique was considered the most appropriate in terms of the accuracy and repeatability to provide the in vitro release kinetics of nanoparticles. Interestingly, the DM method appeared to misestimate the release kinetics of nanoparticles through separate mechanisms. This work offers a superior analytical technique for studying in vitro drug release from polymeric nanoparticles, which could benefit the future development of in vitro-in vivo correlation of polymeric nanoparticles.-
dc.languageeng-
dc.publisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/pharmaceuticals/-
dc.relation.ispartofPharmaceutics-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectpolymeric nanoparticle-
dc.subjectin vitro release kinetics-
dc.subjectcentrifugal ultrafiltration-
dc.subjectdialysis-
dc.subjectfiltration-
dc.titleIn Vitro Release Study of the Polymeric Drug Nanoparticles: Development and Validation of a Novel Method-
dc.typeArticle-
dc.identifier.emailTong, HHY: henry002@hku.hk-
dc.identifier.emailChow, SF: asfchow@hku.hk-
dc.identifier.authorityChow, SF=rp02296-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/pharmaceutics12080732-
dc.identifier.hkuros312595-
dc.identifier.volume12-
dc.identifier.issue8-
dc.identifier.spagearticle no. 732-
dc.identifier.epagearticle no. 732-
dc.publisher.placeSwitzerland-

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