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Article: OPA-Based Bifunctional Linker for Protein Labeling and Profiling

TitleOPA-Based Bifunctional Linker for Protein Labeling and Profiling
Authors
KeywordsAmino acids
Chemical modification
Hydrocarbons
Available lysines
Bi-functional
Issue Date2020
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/biochemistry
Citation
Biochemistry, 2020, v. 59 n. 2, p. 175-178 How to Cite?
AbstractLysine residues have been considered as a routine conjugating site for protein chemical labeling and modification. The commercially available lysine-labeling agents have several limitations in labeling efficiency, stability, and cost. To pursue alternative protein lysine-labeling strategies, herein, we report the development of an ortho-phthalaldehyde (OPA)-based bifunctional linker suitable for protein chemical labeling and profiling. Among three designed OPA-based bifunctional linkers, OPA-NH-alkyne 5 was proved to be optimal for protein labeling with minimal protein turbidity. We further demonstrated OPA-NH-alkyne 5 was applicable for immediate capture of protein or proteome chemical labeling.
Persistent Identifierhttp://hdl.handle.net/10722/284792
ISSN
2019 Impact Factor: 2.865
2015 SCImago Journal Rankings: 1.769
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, Q-
dc.contributor.authorZHANG, Y-
dc.contributor.authorLIU, H-
dc.contributor.authorChow, HY-
dc.contributor.authorTian, R-
dc.contributor.authorFung, YME-
dc.contributor.authorLi, XC-
dc.date.accessioned2020-08-07T09:02:41Z-
dc.date.available2020-08-07T09:02:41Z-
dc.date.issued2020-
dc.identifier.citationBiochemistry, 2020, v. 59 n. 2, p. 175-178-
dc.identifier.issn0006-2960-
dc.identifier.urihttp://hdl.handle.net/10722/284792-
dc.description.abstractLysine residues have been considered as a routine conjugating site for protein chemical labeling and modification. The commercially available lysine-labeling agents have several limitations in labeling efficiency, stability, and cost. To pursue alternative protein lysine-labeling strategies, herein, we report the development of an ortho-phthalaldehyde (OPA)-based bifunctional linker suitable for protein chemical labeling and profiling. Among three designed OPA-based bifunctional linkers, OPA-NH-alkyne 5 was proved to be optimal for protein labeling with minimal protein turbidity. We further demonstrated OPA-NH-alkyne 5 was applicable for immediate capture of protein or proteome chemical labeling.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/biochemistry-
dc.relation.ispartofBiochemistry-
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].-
dc.subjectAmino acids-
dc.subjectChemical modification-
dc.subjectHydrocarbons-
dc.subjectAvailable lysines-
dc.subjectBi-functional-
dc.titleOPA-Based Bifunctional Linker for Protein Labeling and Profiling-
dc.typeArticle-
dc.identifier.emailZhang, Q: qingz@hku.hk-
dc.identifier.emailChow, HY: hchowhy@connect.hku.hk-
dc.identifier.emailFung, YME: eva.fungym@hku.hk-
dc.identifier.emailLi, XC: xuechenl@hku.hk-
dc.identifier.authorityZhang, Q=rp02542-
dc.identifier.authorityFung, YME=rp01986-
dc.identifier.authorityLi, XC=rp00742-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acs.biochem.9b00787-
dc.identifier.pmid31657212-
dc.identifier.scopuseid_2-s2.0-85074882773-
dc.identifier.hkuros311893-
dc.identifier.volume59-
dc.identifier.issue2-
dc.identifier.spage175-
dc.identifier.epage178-
dc.identifier.isiWOS:000509420600010-
dc.publisher.placeUnited States-
dc.identifier.issnl0006-2960-

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