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Article: Single-cell EMT-related transcriptional analysis revealed intra-cluster heterogeneity of tumor cell clusters in epithelial ovarian cancer ascites

TitleSingle-cell EMT-related transcriptional analysis revealed intra-cluster heterogeneity of tumor cell clusters in epithelial ovarian cancer ascites
Authors
Keywordsascites cell
cancer associated fibroblast
cell component
clinical article
epithelial mesenchymal transition
Issue Date2020
PublisherSpringer Nature [academic journals on nature.com]. The Journal's web site is located at http://www.nature.com/onc
Citation
Oncogene, 2020, v. 39, p. 4227-4240 How to Cite?
AbstractMalignant ascites of epithelial ovarian cancer is a metastatic tumor microenvironment in which large amounts of disseminated single cells (DSCs) and disseminated tumor cell clusters (DTCCs) are commonly observed. The tumor cell clusters are known to be more aggressive than individual tumor cells in cancer metastasis; however, little is known about the mechanism. Applying single-cell epithelial-to-mesenchymal transition (EMT)-related transcriptional analysis in 120 DSCs and 195 intra-cluster cells from 27 DTCCs, we demonstrated that DTCCs were heterogeneous cellular units comprised of epithelial tumor cells, leukocytes, and cancer-associated fibroblasts (CAFs). Through the analysis of intra-DTCC heterogeneity, we identified that CAFs induced EMT of tumor cells via TGFβ signaling within the DTCC microenvironment. The activation of EMT program, in particular the upregulation of ZEB2, enabled the acquisition of additional chemoresistance and metastasis abilities of the intra-DTCC tumor cells, which resulted in the aggressiveness of DTCCs.
Persistent Identifierhttp://hdl.handle.net/10722/284135
ISSN
2023 Impact Factor: 6.9
2023 SCImago Journal Rankings: 2.334
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKan, T-
dc.contributor.authorWang, W-
dc.contributor.authorIp, PP-
dc.contributor.authorZhou, S-
dc.contributor.authorWong, AS-
dc.contributor.authorWang, X-
dc.contributor.authorYang, M-
dc.date.accessioned2020-07-20T05:56:22Z-
dc.date.available2020-07-20T05:56:22Z-
dc.date.issued2020-
dc.identifier.citationOncogene, 2020, v. 39, p. 4227-4240-
dc.identifier.issn0950-9232-
dc.identifier.urihttp://hdl.handle.net/10722/284135-
dc.description.abstractMalignant ascites of epithelial ovarian cancer is a metastatic tumor microenvironment in which large amounts of disseminated single cells (DSCs) and disseminated tumor cell clusters (DTCCs) are commonly observed. The tumor cell clusters are known to be more aggressive than individual tumor cells in cancer metastasis; however, little is known about the mechanism. Applying single-cell epithelial-to-mesenchymal transition (EMT)-related transcriptional analysis in 120 DSCs and 195 intra-cluster cells from 27 DTCCs, we demonstrated that DTCCs were heterogeneous cellular units comprised of epithelial tumor cells, leukocytes, and cancer-associated fibroblasts (CAFs). Through the analysis of intra-DTCC heterogeneity, we identified that CAFs induced EMT of tumor cells via TGFβ signaling within the DTCC microenvironment. The activation of EMT program, in particular the upregulation of ZEB2, enabled the acquisition of additional chemoresistance and metastasis abilities of the intra-DTCC tumor cells, which resulted in the aggressiveness of DTCCs.-
dc.languageeng-
dc.publisherSpringer Nature [academic journals on nature.com]. The Journal's web site is located at http://www.nature.com/onc-
dc.relation.ispartofOncogene-
dc.subjectascites cell-
dc.subjectcancer associated fibroblast-
dc.subjectcell component-
dc.subjectclinical article-
dc.subjectepithelial mesenchymal transition-
dc.titleSingle-cell EMT-related transcriptional analysis revealed intra-cluster heterogeneity of tumor cell clusters in epithelial ovarian cancer ascites-
dc.typeArticle-
dc.identifier.emailIp, PP: philipip@hku.hk-
dc.identifier.emailWong, AS: awong1@hku.hk-
dc.identifier.authorityIp, PP=rp01890-
dc.identifier.authorityWong, AS=rp00805-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/s41388-020-1288-2-
dc.identifier.pmid32284541-
dc.identifier.scopuseid_2-s2.0-85083313245-
dc.identifier.hkuros311205-
dc.identifier.volume39-
dc.identifier.spage4227-
dc.identifier.epage4240-
dc.identifier.isiWOS:000526276600001-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl0950-9232-

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