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Article: Fluorescein‐Containing Superoxide Probes with a Modular Copper‐Based Trigger

TitleFluorescein‐Containing Superoxide Probes with a Modular Copper‐Based Trigger
Authors
Keywordscopper
fluorescence
oxidation
reactive oxygen species
superoxide
Issue Date2020
PublisherWiley - V C H Verlag GmbH & Co. KGaA. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%292192-6506
Citation
ChemPlusChem, 2020, v. 85 n. 4, p. 653-658 How to Cite?
AbstractFluorescein‐derived superoxide probes featuring a copper(II) complex that can be activated by superoxide to initiate ether bond cleavage and uncage a fluorescein reporter for imaging in live cells are described. Compared to other superoxide sensing moieties, this bond cleavage strategy can be modularly adapted to fluorescent reporters with different properties without compromising the superoxide reactivity and selectivity. A green‐emitting probe and its lysosome‐targeting analogue have been successfully developed. Both probes are sensitive with more than 30‐fold fluorescence enhancement towards superoxide and are highly selective with no significant response towards other reactive oxygen species. A structure–activity relationship study of the copper‐based superoxide trigger showed that the secondary coordination environment of the copper(II) center is important for the superoxide reactivity and selectivity. The probes have been applied in imaging changes in intracellular superoxide level in live HeLa and HEK293T cells upon menadione stimulation and also in a cellular inflammation model in RAW 264.7 cells.
Persistent Identifierhttp://hdl.handle.net/10722/284010
ISSN
2020 Impact Factor: 2.863
2020 SCImago Journal Rankings: 0.801
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTang, FK-
dc.contributor.authorYu, ZH-
dc.contributor.authorWong, THF-
dc.contributor.authorChung, CYS-
dc.contributor.authorHirao, H-
dc.contributor.authorAu Yeung, HY-
dc.date.accessioned2020-07-20T05:55:18Z-
dc.date.available2020-07-20T05:55:18Z-
dc.date.issued2020-
dc.identifier.citationChemPlusChem, 2020, v. 85 n. 4, p. 653-658-
dc.identifier.issn2192-6506-
dc.identifier.urihttp://hdl.handle.net/10722/284010-
dc.description.abstractFluorescein‐derived superoxide probes featuring a copper(II) complex that can be activated by superoxide to initiate ether bond cleavage and uncage a fluorescein reporter for imaging in live cells are described. Compared to other superoxide sensing moieties, this bond cleavage strategy can be modularly adapted to fluorescent reporters with different properties without compromising the superoxide reactivity and selectivity. A green‐emitting probe and its lysosome‐targeting analogue have been successfully developed. Both probes are sensitive with more than 30‐fold fluorescence enhancement towards superoxide and are highly selective with no significant response towards other reactive oxygen species. A structure–activity relationship study of the copper‐based superoxide trigger showed that the secondary coordination environment of the copper(II) center is important for the superoxide reactivity and selectivity. The probes have been applied in imaging changes in intracellular superoxide level in live HeLa and HEK293T cells upon menadione stimulation and also in a cellular inflammation model in RAW 264.7 cells.-
dc.languageeng-
dc.publisherWiley - V C H Verlag GmbH & Co. KGaA. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%292192-6506-
dc.relation.ispartofChemPlusChem-
dc.rightsThis is the peer reviewed version of the following article: ChemPlusChem, 2020, v. 85 n. 4, p. 653-658, which has been published in final form at https://doi.org/10.1002/cplu.202000059. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectcopper-
dc.subjectfluorescence-
dc.subjectoxidation-
dc.subjectreactive oxygen species-
dc.subjectsuperoxide-
dc.titleFluorescein‐Containing Superoxide Probes with a Modular Copper‐Based Trigger-
dc.typeArticle-
dc.identifier.emailAu Yeung, HY: hoyuay@hku.hk-
dc.identifier.authorityAu Yeung, HY=rp01819-
dc.description.naturepostprint-
dc.identifier.doi10.1002/cplu.202000059-
dc.identifier.pmid32237224-
dc.identifier.scopuseid_2-s2.0-85085579137-
dc.identifier.hkuros310981-
dc.identifier.volume85-
dc.identifier.issue4-
dc.identifier.spage653-
dc.identifier.epage658-
dc.identifier.isiWOS:000536499700008-
dc.publisher.placeGermany-
dc.identifier.issnl2192-6506-

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