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Article: Intracellular Photoactivation of Caspase-3 by Molecular Glues for Spatiotemporal Apoptosis Induction
| Title | Intracellular Photoactivation of Caspase-3 by Molecular Glues for Spatiotemporal Apoptosis Induction |
|---|---|
| Authors | |
| Issue Date | 2020 |
| Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.html |
| Citation | Journal of the American Chemical Society, 2020, v. 142 n. 18, p. 8080-8084 How to Cite? |
| Abstract | Caspase-3 (Casp-3) is an enzyme that efficiently induces apoptosis, a form of programmed cell death. We report a dendritic molecular glue PCGlue that enables intracellular delivery of Casp-3 and its photoactivation. PCGlue carrying multiple guanidinium (Gu+) ion pendants via photocleavable linkages can tightly adhere to Casp-3 and deliver it into the cytoplasm mainly by direct penetration through the plasma membrane. Casp-3, whose surface is covered by PCGlue, is unable to interact with its cellular substrates and can therefore not induce apoptosis. However, upon exposure to UV or two-photon near-infrared (NIR) light, PCGlue is cleaved off to liberate Casp-3, triggering the apoptotic signaling cascade. This intracellular photoactivation of Casp-3 allows spatiotemporal induction of apoptosis in irradiated cells. |
| Persistent Identifier | http://hdl.handle.net/10722/282878 |
| ISSN | 2023 Impact Factor: 14.4 2023 SCImago Journal Rankings: 5.489 |
| ISI Accession Number ID |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Hentzen, NB | - |
| dc.contributor.author | Mogaki, R | - |
| dc.contributor.author | Otake, S | - |
| dc.contributor.author | Okuro, K | - |
| dc.contributor.author | Aida, T | - |
| dc.date.accessioned | 2020-06-05T06:22:37Z | - |
| dc.date.available | 2020-06-05T06:22:37Z | - |
| dc.date.issued | 2020 | - |
| dc.identifier.citation | Journal of the American Chemical Society, 2020, v. 142 n. 18, p. 8080-8084 | - |
| dc.identifier.issn | 0002-7863 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/282878 | - |
| dc.description.abstract | Caspase-3 (Casp-3) is an enzyme that efficiently induces apoptosis, a form of programmed cell death. We report a dendritic molecular glue <font size=-1><sup>PC</sup></font>Glue that enables intracellular delivery of Casp-3 and its photoactivation. <font size=-1><sup>PC</sup></font>Glue carrying multiple guanidinium (Gu<font size=-1><sup>+</sup></font>) ion pendants via photocleavable linkages can tightly adhere to Casp-3 and deliver it into the cytoplasm mainly by direct penetration through the plasma membrane. Casp-3, whose surface is covered by <font size=-1><sup>PC</sup></font>Glue, is unable to interact with its cellular substrates and can therefore not induce apoptosis. However, upon exposure to UV or two-photon near-infrared (NIR) light, <font size=-1><sup>PC</sup></font>Glue is cleaved off to liberate Casp-3, triggering the apoptotic signaling cascade. This intracellular photoactivation of Casp-3 allows spatiotemporal induction of apoptosis in irradiated cells. | - |
| dc.language | eng | - |
| dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.html | - |
| dc.relation.ispartof | Journal of the American Chemical Society | - |
| dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of the American Chemical Society, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/jacs.0c01823 | - |
| dc.title | Intracellular Photoactivation of Caspase-3 by Molecular Glues for Spatiotemporal Apoptosis Induction | - |
| dc.type | Article | - |
| dc.identifier.email | Okuro, K: okuro@hku.hk | - |
| dc.identifier.authority | Okuro, K=rp02557 | - |
| dc.description.nature | postprint | - |
| dc.identifier.doi | 10.1021/jacs.0c01823 | - |
| dc.identifier.scopus | eid_2-s2.0-85085131137 | - |
| dc.identifier.hkuros | 310127 | - |
| dc.identifier.volume | 142 | - |
| dc.identifier.issue | 18 | - |
| dc.identifier.spage | 8080 | - |
| dc.identifier.epage | 8084 | - |
| dc.identifier.isi | WOS:000535173500005 | - |
| dc.publisher.place | United States | - |
| dc.identifier.issnl | 0002-7863 | - |