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Article: Identification of a Novel Betacoronavirus (Merbecovirus) in Amur Hedgehogs from China

TitleIdentification of a Novel Betacoronavirus (Merbecovirus) in Amur Hedgehogs from China
Authors
Keywordshedgehog
Merbecovirus
coronavirus
novel species
China
Issue Date2019
PublisherMolecular Diversity Preservation International (MDPI) AG.. The Journal's web site is located at http://www.mdpi.com/journal/viruses
Citation
Viruses, 2019, v. 11 n. 11, p. article no. 980 How to Cite?
AbstractWhile dromedaries are the immediate animal source of Middle East Respiratory Syndrome (MERS) epidemic, viruses related to MERS coronavirus (MERS-CoV) have also been found in bats as well as hedgehogs. To elucidate the evolution of MERS-CoV-related viruses and their interspecies transmission pathway, samples were collected from different mammals in China. A novel coronavirus related to MERS-CoV, Erinaceus amurensis hedgehog coronavirus HKU31 (Ea-HedCoV HKU31), was identified from two Amur hedgehogs. Genome analysis supported that Ea-HedCoV HKU31 represents a novel species under Merbecovirus, being most closely related to Erinaceus CoV from European hedgehogs in Germany, with 79.6% genome sequence identity. Compared to other members of Merbecovirus, Ea-HedCoV HKU31 possessed unique non-structural proteins and putative cleavage sites at ORF1ab. Phylogenetic analysis showed that Ea-HedCoV HKU31 and BetaCoV Erinaceus/VMC/DEU/2012 were closely related to NeoCoV and BatCoV PREDICT from African bats in the spike region, suggesting that the latter bat viruses have arisen from recombination between CoVs from hedgehogs and bats. The predicted HKU31 receptor-binding domain (RBD) possessed only one out of 12 critical amino acid residues for binding to human dipeptidyl peptidase 4 (hDPP4), the MERS-CoV receptor. The structural modeling of the HKU31-RBD-hDPP4 binding interphase compared to that of MERS-CoV and Tylonycteris bat CoV HKU4 (Ty-BatCoV HKU4) suggested that HKU31-RBD is unlikely to bind to hDPP4. Our findings support that hedgehogs are an important reservoir of Merbecovirus, with evidence of recombination with viruses from bats. Further investigations in bats, hedgehogs and related animals are warranted to understand the evolution of MERS-CoV-related viruses.
Descriptioneid_2-s2.0-85074139277
Persistent Identifierhttp://hdl.handle.net/10722/281889
ISSN
2018 Impact Factor: 3.811
2015 SCImago Journal Rankings: 1.873
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorLau, SKP-
dc.contributor.authorLuk, HKH-
dc.contributor.authorWong, ACP-
dc.contributor.authorFan, RYY-
dc.contributor.authorLam, CSF-
dc.contributor.authorLi, KSM-
dc.contributor.authorAhmed, SS-
dc.contributor.authorChow, FWN-
dc.contributor.authorCai, J-P-
dc.contributor.authorZhu, X-
dc.contributor.authorChan, JFW-
dc.contributor.authorLau, TCK-
dc.contributor.authorCao, K-
dc.contributor.authorLi, M-
dc.contributor.authorWoo, PCY-
dc.contributor.authorYuen, K-Y-
dc.date.accessioned2020-04-03T07:23:12Z-
dc.date.available2020-04-03T07:23:12Z-
dc.date.issued2019-
dc.identifier.citationViruses, 2019, v. 11 n. 11, p. article no. 980-
dc.identifier.issn1999-4915-
dc.identifier.urihttp://hdl.handle.net/10722/281889-
dc.descriptioneid_2-s2.0-85074139277-
dc.description.abstractWhile dromedaries are the immediate animal source of Middle East Respiratory Syndrome (MERS) epidemic, viruses related to MERS coronavirus (MERS-CoV) have also been found in bats as well as hedgehogs. To elucidate the evolution of MERS-CoV-related viruses and their interspecies transmission pathway, samples were collected from different mammals in China. A novel coronavirus related to MERS-CoV, Erinaceus amurensis hedgehog coronavirus HKU31 (Ea-HedCoV HKU31), was identified from two Amur hedgehogs. Genome analysis supported that Ea-HedCoV HKU31 represents a novel species under Merbecovirus, being most closely related to Erinaceus CoV from European hedgehogs in Germany, with 79.6% genome sequence identity. Compared to other members of Merbecovirus, Ea-HedCoV HKU31 possessed unique non-structural proteins and putative cleavage sites at ORF1ab. Phylogenetic analysis showed that Ea-HedCoV HKU31 and BetaCoV Erinaceus/VMC/DEU/2012 were closely related to NeoCoV and BatCoV PREDICT from African bats in the spike region, suggesting that the latter bat viruses have arisen from recombination between CoVs from hedgehogs and bats. The predicted HKU31 receptor-binding domain (RBD) possessed only one out of 12 critical amino acid residues for binding to human dipeptidyl peptidase 4 (hDPP4), the MERS-CoV receptor. The structural modeling of the HKU31-RBD-hDPP4 binding interphase compared to that of MERS-CoV and Tylonycteris bat CoV HKU4 (Ty-BatCoV HKU4) suggested that HKU31-RBD is unlikely to bind to hDPP4. Our findings support that hedgehogs are an important reservoir of Merbecovirus, with evidence of recombination with viruses from bats. Further investigations in bats, hedgehogs and related animals are warranted to understand the evolution of MERS-CoV-related viruses.-
dc.languageeng-
dc.publisherMolecular Diversity Preservation International (MDPI) AG.. The Journal's web site is located at http://www.mdpi.com/journal/viruses-
dc.relation.ispartofViruses-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjecthedgehog-
dc.subjectMerbecovirus-
dc.subjectcoronavirus-
dc.subjectnovel species-
dc.subjectChina-
dc.titleIdentification of a Novel Betacoronavirus (Merbecovirus) in Amur Hedgehogs from China-
dc.typeArticle-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailLuk, HKH: hkhluk@hku.hk-
dc.identifier.emailLi, KSM: kenn105@hkucc.hku.hk-
dc.identifier.emailChow, FWN: chow5810@hku.hk-
dc.identifier.emailCai, J-P: caijuice@hku.hk-
dc.identifier.emailChan, JFW: jfwchan@hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.emailYuen, K-Y: kyyuen@hkucc.hku.hk-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityChow, FWN=rp02493-
dc.identifier.authorityChan, JFW=rp01736-
dc.identifier.authorityWoo, PCY=rp00430-
dc.identifier.authorityYuen, K-Y=rp00366-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/v11110980-
dc.identifier.pmid31653070-
dc.identifier.pmcidPMC6893546-
dc.identifier.scopuseid_2-s2.0-85074139277-
dc.identifier.hkuros309639-
dc.identifier.volume11-
dc.identifier.issue11-
dc.identifier.spagearticle no. 980-
dc.identifier.epagearticle no. 980-
dc.publisher.placeSwitzerland-

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