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Article: Prolonged UV-C Irradiation is a Double-Edged Sword on the Zirconia Surface

TitleProlonged UV-C Irradiation is a Double-Edged Sword on the Zirconia Surface
Authors
KeywordsBiocompatibility
Cell adhesion
Cell proliferation
Color
Contact angle
Issue Date2020
PublisherAmerican Chemical Society: Open Access Titles. The Journal's web site is located at http://pubs.acs.org/journal/acsodf
Citation
ACS Omega, 2020, v. 5 n. 10, p. 5126-5133 How to Cite?
AbstractZirconia has become an excellent choice of dental implants because of its excellent mechanical strength, aesthetic, and biocompatibility. Although some studies have shown ultraviolet (UV) irradiation is effective to photofunctionalize dental zirconia that can improve osteoblastic function, the scattered information has not identified the most effective exposure time and wavelength of UV. Herein, this study has investigated the effects of UV irradiation on zirconia after UV-A (365 nm) or UV-C (243 nm) photofunctionalization for different times (15 min, 3 and 24 h). After irradiation, the zirconia surface was analyzed by color spectrophotometry, scanned electron microscopy (SEM), energy-dispersive X-ray spectrometry, water contact angle (WCA) with goniometer, and X-ray diffraction. Osteoblastic (MC3T3-E1) cells were cultured on zirconia discs and evaluated with a CCK-8 test kit for cell proliferation (3 h and 1 day) and with alkaline phosphatase (ALP) activity (14 days). Significant color change (ΔE) was observed by irradiating with UV-C for 15 min (1.99), 3 h (1.92), and 24 h (3.35), whereas only minute changes were observed with UV-A (respectively, ΔE: 0.18, 0.14, 0.57). No surface textural changes were observed nor a monoclinic phase was detected on both the UV-A and UV-C irradiated samples. UV-C significantly decreased the C/Zr ratios and WCA, with irradiating for 24 h presenting the lowest values, and it was the only condition to give significantly higher ALP activity at 14 days (p < 0.05) and CCK-8 values for 1 day culture (p < 0.05). It is concluded that UV-C (but not UV-A) irradiation can significantly change the aesthetic in color, and only prolonged 24 h UV-C irradiation can enhance MC3T3-E1 cell adhesion on zirconia by photofunctionalization.
Persistent Identifierhttp://hdl.handle.net/10722/281789
ISSN
2023 Impact Factor: 3.7
2023 SCImago Journal Rankings: 0.710
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHAN, A-
dc.contributor.authorDING, H-
dc.contributor.authorTsoi, JKH-
dc.contributor.authorImazato, S-
dc.contributor.authorMatinlinna, JP-
dc.contributor.authorChen, Z-F-
dc.date.accessioned2020-03-27T04:22:32Z-
dc.date.available2020-03-27T04:22:32Z-
dc.date.issued2020-
dc.identifier.citationACS Omega, 2020, v. 5 n. 10, p. 5126-5133-
dc.identifier.issn2470-1343-
dc.identifier.urihttp://hdl.handle.net/10722/281789-
dc.description.abstractZirconia has become an excellent choice of dental implants because of its excellent mechanical strength, aesthetic, and biocompatibility. Although some studies have shown ultraviolet (UV) irradiation is effective to photofunctionalize dental zirconia that can improve osteoblastic function, the scattered information has not identified the most effective exposure time and wavelength of UV. Herein, this study has investigated the effects of UV irradiation on zirconia after UV-A (365 nm) or UV-C (243 nm) photofunctionalization for different times (15 min, 3 and 24 h). After irradiation, the zirconia surface was analyzed by color spectrophotometry, scanned electron microscopy (SEM), energy-dispersive X-ray spectrometry, water contact angle (WCA) with goniometer, and X-ray diffraction. Osteoblastic (MC3T3-E1) cells were cultured on zirconia discs and evaluated with a CCK-8 test kit for cell proliferation (3 h and 1 day) and with alkaline phosphatase (ALP) activity (14 days). Significant color change (ΔE) was observed by irradiating with UV-C for 15 min (1.99), 3 h (1.92), and 24 h (3.35), whereas only minute changes were observed with UV-A (respectively, ΔE: 0.18, 0.14, 0.57). No surface textural changes were observed nor a monoclinic phase was detected on both the UV-A and UV-C irradiated samples. UV-C significantly decreased the C/Zr ratios and WCA, with irradiating for 24 h presenting the lowest values, and it was the only condition to give significantly higher ALP activity at 14 days (p < 0.05) and CCK-8 values for 1 day culture (p < 0.05). It is concluded that UV-C (but not UV-A) irradiation can significantly change the aesthetic in color, and only prolonged 24 h UV-C irradiation can enhance MC3T3-E1 cell adhesion on zirconia by photofunctionalization.-
dc.languageeng-
dc.publisherAmerican Chemical Society: Open Access Titles. The Journal's web site is located at http://pubs.acs.org/journal/acsodf-
dc.relation.ispartofACS Omega-
dc.rightsThis is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectBiocompatibility-
dc.subjectCell adhesion-
dc.subjectCell proliferation-
dc.subjectColor-
dc.subjectContact angle-
dc.titleProlonged UV-C Irradiation is a Double-Edged Sword on the Zirconia Surface-
dc.typeArticle-
dc.identifier.emailTsoi, JKH: jkhtsoi@hku.hk-
dc.identifier.emailMatinlinna, JP: jpmat@hku.hk-
dc.identifier.authorityTsoi, JKH=rp01609-
dc.identifier.authorityMatinlinna, JP=rp00052-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1021/acsomega.9b04123-
dc.identifier.scopuseid_2-s2.0-85081648685-
dc.identifier.hkuros309535-
dc.identifier.volume5-
dc.identifier.issue10-
dc.identifier.spage5126-
dc.identifier.epage5133-
dc.identifier.isiWOS:000520853400045-
dc.publisher.placeUnited States-
dc.identifier.issnl2470-1343-

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