File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1073/pnas.1916164117
- Scopus: eid_2-s2.0-85080044390
- PMID: 32029586
- WOS: WOS:000516771500045
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Fbxo9 functions downstream of Sox10 to determine neuron-glial fate choice in the dorsal root ganglia through Neurog2 destabilization
Title | Fbxo9 functions downstream of Sox10 to determine neuron-glial fate choice in the dorsal root ganglia through Neurog2 destabilization |
---|---|
Authors | |
Keywords | Sox10 Neurog2 Fbxo9 neural crest progenitors |
Issue Date | 2020 |
Publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org |
Citation | Proceedings of the National Academy of Sciences, 2020, v. 117 n. 8, p. 4199-4210 How to Cite? |
Abstract | The transcription factor Sox10 is a key regulator in the fate determination of a subpopulation of multipotent trunk neural crest (NC) progenitors toward glial cells instead of sensory neurons in the dorsal root ganglia (DRG). However, the mechanism by which Sox10 regulates glial cell fate commitment during lineage segre- gation remains poorly understood. In our study, we showed that the neurogenic determinant Neurogenin 2 (Neurog2) exhibited tran- sient overlapping expression with Sox10 in avian trunk NC progenitors, which progressively underwent lineage segregation during mi- gration toward the forming DRG. Gain- and loss-of-function studies revealed that the temporary expression of Neurog2 was due to Sox10 regulation of its protein stability. Transcriptional profiling identified Sox10-regulated F-box only protein (Fbxo9), which is an SCF (Skp1-Cul-F-box)-type ubiquitin ligase for Neurog2. Con- sistently, overexpression of Fbxo9 in NC progenitors down- regulated Neurog2 protein expression through ubiquitination and promoted the glial lineage at the expense of neuronal differ- entiation, whereas Fbxo9 knockdown resulted in the opposite phenomenon. Mechanistically, we found that Fbxo9 interacted with Neurog2 to promote its destabilization through the F-box motif. Finally, epistasis analysis further demonstrated that Fbxo9 and probably other F-box members mediated the role of Sox10 in destabilizing Neurog2 protein and directing the lineage of NC progenitors toward glial cells rather than sensory neurons. Altogether, these findings unravel a Sox10–Fbxo9 regulatory axis in promoting the glial fate of NC progenitors through Neurog2 destabilization. |
Persistent Identifier | http://hdl.handle.net/10722/281211 |
ISSN | 2023 Impact Factor: 9.4 2023 SCImago Journal Rankings: 3.737 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liu, JA | - |
dc.contributor.author | Tai, A | - |
dc.contributor.author | Hong, J-L | - |
dc.contributor.author | Cheung, MPL | - |
dc.contributor.author | Sham, MH | - |
dc.contributor.author | Cheah, KSE | - |
dc.contributor.author | Cheung, CW | - |
dc.contributor.author | Cheung, M | - |
dc.date.accessioned | 2020-03-09T09:51:36Z | - |
dc.date.available | 2020-03-09T09:51:36Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences, 2020, v. 117 n. 8, p. 4199-4210 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10722/281211 | - |
dc.description.abstract | The transcription factor Sox10 is a key regulator in the fate determination of a subpopulation of multipotent trunk neural crest (NC) progenitors toward glial cells instead of sensory neurons in the dorsal root ganglia (DRG). However, the mechanism by which Sox10 regulates glial cell fate commitment during lineage segre- gation remains poorly understood. In our study, we showed that the neurogenic determinant Neurogenin 2 (Neurog2) exhibited tran- sient overlapping expression with Sox10 in avian trunk NC progenitors, which progressively underwent lineage segregation during mi- gration toward the forming DRG. Gain- and loss-of-function studies revealed that the temporary expression of Neurog2 was due to Sox10 regulation of its protein stability. Transcriptional profiling identified Sox10-regulated F-box only protein (Fbxo9), which is an SCF (Skp1-Cul-F-box)-type ubiquitin ligase for Neurog2. Con- sistently, overexpression of Fbxo9 in NC progenitors down- regulated Neurog2 protein expression through ubiquitination and promoted the glial lineage at the expense of neuronal differ- entiation, whereas Fbxo9 knockdown resulted in the opposite phenomenon. Mechanistically, we found that Fbxo9 interacted with Neurog2 to promote its destabilization through the F-box motif. Finally, epistasis analysis further demonstrated that Fbxo9 and probably other F-box members mediated the role of Sox10 in destabilizing Neurog2 protein and directing the lineage of NC progenitors toward glial cells rather than sensory neurons. Altogether, these findings unravel a Sox10–Fbxo9 regulatory axis in promoting the glial fate of NC progenitors through Neurog2 destabilization. | - |
dc.language | eng | - |
dc.publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org | - |
dc.relation.ispartof | Proceedings of the National Academy of Sciences | - |
dc.rights | Proceedings of the National Academy of Sciences. Copyright © National Academy of Sciences. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Sox10 | - |
dc.subject | Neurog2 | - |
dc.subject | Fbxo9 | - |
dc.subject | neural crest progenitors | - |
dc.title | Fbxo9 functions downstream of Sox10 to determine neuron-glial fate choice in the dorsal root ganglia through Neurog2 destabilization | - |
dc.type | Article | - |
dc.identifier.email | Liu, JA: jessie11@hku.hk | - |
dc.identifier.email | Tai, A: cpandrew@hku.hk | - |
dc.identifier.email | Cheung, MPL: mplcheun@hku.hk | - |
dc.identifier.email | Sham, MH: mhsham@hku.hk | - |
dc.identifier.email | Cheah, KSE: hrmbdkc@hku.hk | - |
dc.identifier.email | Cheung, CW: cheucw@hku.hk | - |
dc.identifier.email | Cheung, M: mcheung9@hku.hk | - |
dc.identifier.authority | Liu, JA=rp02546 | - |
dc.identifier.authority | Sham, MH=rp00380 | - |
dc.identifier.authority | Cheah, KSE=rp00342 | - |
dc.identifier.authority | Cheung, CW=rp00244 | - |
dc.identifier.authority | Cheung, M=rp00245 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1073/pnas.1916164117 | - |
dc.identifier.pmid | 32029586 | - |
dc.identifier.pmcid | PMC7049171 | - |
dc.identifier.scopus | eid_2-s2.0-85080044390 | - |
dc.identifier.hkuros | 309299 | - |
dc.identifier.volume | 117 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 4199 | - |
dc.identifier.epage | 4210 | - |
dc.identifier.isi | WOS:000516771500045 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0027-8424 | - |