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- Publisher Website: 10.1101/gr.225615.117
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Article: Transcription rate strongly affects splicing fidelity and cotranscriptionality in budding yeast
Title | Transcription rate strongly affects splicing fidelity and cotranscriptionality in budding yeast |
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Authors | |
Issue Date | 2018 |
Citation | Genome Research, 2018, v. 28, n. 2, p. 203-213 How to Cite? |
Abstract | © 2018 Aslanzadeh et al. The functional consequences of alternative splicing on altering the transcription rate have been the subject of intensive study in mammalian cells but less is known about effects of splicing on changing the transcription rate in yeast. We present several lines of evidence showing that slow RNA polymerase II elongation increases both cotranscriptional splicing and splicing efficiency and that faster elongation reduces cotranscriptional splicing and splicing efficiency in budding yeast, suggesting that splicing is more efficient when cotranscriptional. Moreover, we demonstrate that altering the RNA polymerase II elongation rate in either direction compromises splicing fidelity, and we reveal that splicing fidelity depends largely on intron length together with secondary structure and splice site score. These effects are notably stronger for the highly expressed ribosomal protein coding transcripts. We propose that transcription by RNA polymerase II is tuned to optimize the efficiency and accuracy of ribosomal protein gene expression, while allowing flexibility in splice site choice with the non-ribosomal protein transcripts. |
Persistent Identifier | http://hdl.handle.net/10722/280649 |
ISSN | 2023 Impact Factor: 6.2 2023 SCImago Journal Rankings: 4.403 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Aslanzadeh, Vahid | - |
dc.contributor.author | Huang, Yuanhua | - |
dc.contributor.author | Sanguinetti, Guido | - |
dc.contributor.author | Beggs, Jean D. | - |
dc.date.accessioned | 2020-02-17T14:34:34Z | - |
dc.date.available | 2020-02-17T14:34:34Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Genome Research, 2018, v. 28, n. 2, p. 203-213 | - |
dc.identifier.issn | 1088-9051 | - |
dc.identifier.uri | http://hdl.handle.net/10722/280649 | - |
dc.description.abstract | © 2018 Aslanzadeh et al. The functional consequences of alternative splicing on altering the transcription rate have been the subject of intensive study in mammalian cells but less is known about effects of splicing on changing the transcription rate in yeast. We present several lines of evidence showing that slow RNA polymerase II elongation increases both cotranscriptional splicing and splicing efficiency and that faster elongation reduces cotranscriptional splicing and splicing efficiency in budding yeast, suggesting that splicing is more efficient when cotranscriptional. Moreover, we demonstrate that altering the RNA polymerase II elongation rate in either direction compromises splicing fidelity, and we reveal that splicing fidelity depends largely on intron length together with secondary structure and splice site score. These effects are notably stronger for the highly expressed ribosomal protein coding transcripts. We propose that transcription by RNA polymerase II is tuned to optimize the efficiency and accuracy of ribosomal protein gene expression, while allowing flexibility in splice site choice with the non-ribosomal protein transcripts. | - |
dc.language | eng | - |
dc.relation.ispartof | Genome Research | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Transcription rate strongly affects splicing fidelity and cotranscriptionality in budding yeast | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1101/gr.225615.117 | - |
dc.identifier.pmid | 29254943 | - |
dc.identifier.pmcid | PMC5793784 | - |
dc.identifier.scopus | eid_2-s2.0-85041488372 | - |
dc.identifier.volume | 28 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 203 | - |
dc.identifier.epage | 213 | - |
dc.identifier.eissn | 1549-5469 | - |
dc.identifier.isi | WOS:000424537000006 | - |
dc.identifier.issnl | 1088-9051 | - |