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Article: Small RNA profiling of piRNAs in colorectal cancer identifies consistent overexpression of piR-24000 that correlates clinically with an aggressive disease phenotype
Title | Small RNA profiling of piRNAs in colorectal cancer identifies consistent overexpression of piR-24000 that correlates clinically with an aggressive disease phenotype |
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Authors | |
Keywords | piRNA colorectal cancer prognosis piR-24000 non-coding RNA |
Issue Date | 2020 |
Publisher | MDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/cancers/ |
Citation | Cancers, 2020, v. 12 n. 1, article no. 188 How to Cite? |
Abstract | Piwi-interacting RNAs (piRNAs) represent a novel class of small non-coding RNAs (ncRNAs) that have been shown to have a deregulated expression in several cancers, although their clinical significance in colorectal cancer (CRC) remains unclear. With an aim of delineating the piRNA distribution in CRC, we conducted a systematic discovery and validation of piRNAs within two clinical cohorts. In the discovery phase, we profiled tumor and adjacent normal tissues from 18 CRC patients by deep sequencing and identified a global piRNA downregulation in CRC. Moreover, we identified piR-24000 as an unexplored piRNA that was significantly overexpressed in CRC. Using qPCR, we validated the overexpression of piR-24000 in 87 CRC patients. Additionally, we identified a significant association between a high expression of piR-24000 and an aggressive CRC phenotype including poor differentiation, presence of distant metastases, and a higher stage. Lastly, ROC analysis demonstrated a strong diagnostic power of piR-24000 in discriminating CRC patients from normal subjects. Taken together, this study provides one of the earliest large-scale reports of the global distribution of piRNAs in CRC. In addition, piR-24000 was identified as a likely oncogene in CRC that can serve as a biomarker or a therapeutic target. |
Persistent Identifier | http://hdl.handle.net/10722/280319 |
ISSN | 2023 Impact Factor: 4.5 2023 SCImago Journal Rankings: 1.391 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Iyer, DN | - |
dc.contributor.author | Wan, TMH | - |
dc.contributor.author | Man, JHW | - |
dc.contributor.author | Sin, RWY | - |
dc.contributor.author | Li, X | - |
dc.contributor.author | Lo, OSH | - |
dc.contributor.author | Foo, DCC | - |
dc.contributor.author | Pang, RWC | - |
dc.contributor.author | Law, WL | - |
dc.contributor.author | Ng, L | - |
dc.date.accessioned | 2020-02-07T07:39:27Z | - |
dc.date.available | 2020-02-07T07:39:27Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Cancers, 2020, v. 12 n. 1, article no. 188 | - |
dc.identifier.issn | 2072-6694 | - |
dc.identifier.uri | http://hdl.handle.net/10722/280319 | - |
dc.description.abstract | Piwi-interacting RNAs (piRNAs) represent a novel class of small non-coding RNAs (ncRNAs) that have been shown to have a deregulated expression in several cancers, although their clinical significance in colorectal cancer (CRC) remains unclear. With an aim of delineating the piRNA distribution in CRC, we conducted a systematic discovery and validation of piRNAs within two clinical cohorts. In the discovery phase, we profiled tumor and adjacent normal tissues from 18 CRC patients by deep sequencing and identified a global piRNA downregulation in CRC. Moreover, we identified piR-24000 as an unexplored piRNA that was significantly overexpressed in CRC. Using qPCR, we validated the overexpression of piR-24000 in 87 CRC patients. Additionally, we identified a significant association between a high expression of piR-24000 and an aggressive CRC phenotype including poor differentiation, presence of distant metastases, and a higher stage. Lastly, ROC analysis demonstrated a strong diagnostic power of piR-24000 in discriminating CRC patients from normal subjects. Taken together, this study provides one of the earliest large-scale reports of the global distribution of piRNAs in CRC. In addition, piR-24000 was identified as a likely oncogene in CRC that can serve as a biomarker or a therapeutic target. | - |
dc.language | eng | - |
dc.publisher | MDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/cancers/ | - |
dc.relation.ispartof | Cancers | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | piRNA | - |
dc.subject | colorectal cancer | - |
dc.subject | prognosis | - |
dc.subject | piR-24000 | - |
dc.subject | non-coding RNA | - |
dc.title | Small RNA profiling of piRNAs in colorectal cancer identifies consistent overexpression of piR-24000 that correlates clinically with an aggressive disease phenotype | - |
dc.type | Article | - |
dc.identifier.email | Iyer, DN: deeiyer@hku.hk | - |
dc.identifier.email | Wan, TMH: tmhwan@hku.hk | - |
dc.identifier.email | Sin, RWY: wysin17@HKUCC-COM.hku.hk | - |
dc.identifier.email | Li, X: daisyxue@hku.hk | - |
dc.identifier.email | Lo, OSH: oswens@hku.hk | - |
dc.identifier.email | Foo, DCC: ccfoo@hku.hk | - |
dc.identifier.email | Law, WL: lawwl@hkucc.hku.hk | - |
dc.identifier.email | Ng, L: luing@hku.hk | - |
dc.identifier.authority | Foo, DCC=rp01899 | - |
dc.identifier.authority | Law, WL=rp00436 | - |
dc.identifier.authority | Ng, L=rp02207 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3390/cancers12010188 | - |
dc.identifier.scopus | eid_2-s2.0-85078234318 | - |
dc.identifier.hkuros | 309135 | - |
dc.identifier.volume | 12 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. 188 | - |
dc.identifier.epage | article no. 188 | - |
dc.identifier.isi | WOS:000516826700188 | - |
dc.publisher.place | Switzerland | - |
dc.identifier.issnl | 2072-6694 | - |