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Article: High siRNA loading powder for inhalation prepared by co-spray drying with human serum albumin

TitleHigh siRNA loading powder for inhalation prepared by co-spray drying with human serum albumin
Authors
KeywordsDesign of experiment
Dry powder
Human serum albumin
Pulmonary delivery
Small interfering RNA
Issue Date2019
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijpharm
Citation
International Journal of Pharmaceutics, 2019, v. 572, p. article no. 118818 How to Cite?
AbstractThe development of small interfering RNA (siRNA) formulation for pulmonary delivery is a key to the clinical translation of siRNA therapeutics for the treatment of respiratory diseases. Most inhalable siRNA powder formulations published to date were limited by the siRNA content which was often too low to be clinically relevant. This study aimed to prepare inhalable siRNA powder formulations that contained high siRNA loading of over 6% w/w by spray drying, with human serum albumin (HSA) investigated as a dispersion enhancer to improve the aerosol performance. The effect of siRNA, HSA and solute concentrations in the formulations were evaluated systemically using factorial analyses. All the spray dried siRNA powders exhibited excellent aerosol performance with fine particle fraction (FPF) consistently over 50% in all the formulations. An enrichment of HSA on the particle surface was observed. Surface corrugation was more prominent as HSA composition increased. Importantly, the bioactivity of siRNA was successfully preserved upon spray drying as demonstrated in the in vitro transfection study, and up to 78% of intact siRNA retained in the spray dried powder. Overall, HSA is an effective dispersion enhancer and spray drying is an appropriate technique to produce inhalable dry powder with high siRNA loading for further investigation.
Persistent Identifierhttp://hdl.handle.net/10722/280091
ISSN
2019 Impact Factor: 4.845
2015 SCImago Journal Rankings: 1.315

 

DC FieldValueLanguage
dc.contributor.authorCHOW, YT-
dc.contributor.authorQIU, Y-
dc.contributor.authorLIAO, Q-
dc.contributor.authorKwok, PCL-
dc.contributor.authorChow, SF-
dc.contributor.authorChan, HK-
dc.contributor.authorLam, JKW-
dc.date.accessioned2020-01-06T02:00:50Z-
dc.date.available2020-01-06T02:00:50Z-
dc.date.issued2019-
dc.identifier.citationInternational Journal of Pharmaceutics, 2019, v. 572, p. article no. 118818-
dc.identifier.issn0378-5173-
dc.identifier.urihttp://hdl.handle.net/10722/280091-
dc.description.abstractThe development of small interfering RNA (siRNA) formulation for pulmonary delivery is a key to the clinical translation of siRNA therapeutics for the treatment of respiratory diseases. Most inhalable siRNA powder formulations published to date were limited by the siRNA content which was often too low to be clinically relevant. This study aimed to prepare inhalable siRNA powder formulations that contained high siRNA loading of over 6% w/w by spray drying, with human serum albumin (HSA) investigated as a dispersion enhancer to improve the aerosol performance. The effect of siRNA, HSA and solute concentrations in the formulations were evaluated systemically using factorial analyses. All the spray dried siRNA powders exhibited excellent aerosol performance with fine particle fraction (FPF) consistently over 50% in all the formulations. An enrichment of HSA on the particle surface was observed. Surface corrugation was more prominent as HSA composition increased. Importantly, the bioactivity of siRNA was successfully preserved upon spray drying as demonstrated in the in vitro transfection study, and up to 78% of intact siRNA retained in the spray dried powder. Overall, HSA is an effective dispersion enhancer and spray drying is an appropriate technique to produce inhalable dry powder with high siRNA loading for further investigation.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijpharm-
dc.relation.ispartofInternational Journal of Pharmaceutics-
dc.subjectDesign of experiment-
dc.subjectDry powder-
dc.subjectHuman serum albumin-
dc.subjectPulmonary delivery-
dc.subjectSmall interfering RNA-
dc.titleHigh siRNA loading powder for inhalation prepared by co-spray drying with human serum albumin-
dc.typeArticle-
dc.identifier.emailKwok, PCL: pclkwok@hku.hk-
dc.identifier.emailChow, SF: asfchow@hku.hk-
dc.identifier.emailLam, JKW: jkwlam@hku.hk-
dc.identifier.authorityKwok, PCL=rp01540-
dc.identifier.authorityChow, SF=rp02296-
dc.identifier.authorityLam, JKW=rp01346-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ijpharm.2019.118818-
dc.identifier.pmid31678379-
dc.identifier.scopuseid_2-s2.0-85075396326-
dc.identifier.hkuros308909-
dc.identifier.volume572-
dc.identifier.spagearticle no. 118818-
dc.identifier.epagearticle no. 118818-
dc.publisher.placeNetherlands-

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