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Article: Periodontal commensals and pathogens differentially modulate immuno-inflammatory response in human oral keratinocytes

TitlePeriodontal commensals and pathogens differentially modulate immuno-inflammatory response in human oral keratinocytes
Authors
Keywordscommensals
periodontopathogens
human oral keratinocytes
bacteria-host interaction
cytokines
Issue Date2019
PublisherQuintessence Publishing Co Ltd. The Journal's web site is located at http://www.quintpub.com/journals/cjdr/gp.php?journal_name=CJDR&name_abbr=CJDR
Citation
The Chinese Journal of Dental Research, 2019, v. 22 n. 2, p. 105-112 How to Cite?
AbstractObjective: To investigate the immunoinflammatory response in the crosstalk of human oral keratinocytes (HOKs) with selected periodontal commensals and pathogens. Methods: Four representative viable oral bacteria, including periodontal commensals (Streptococcus mutans, Sm; and Actinomyces israelii, Ai) and pathogens (Aggregatibacter actinomycetemcomitans, Aa; and Porphyromonas gingivalis, Pg), were selected. A viable bacteria-HOKs interactive model was tested under various conditions of oxygen, antibiotics, duration and multiplicity of infection (MOI). The expression of IL-6 and IL-8 in HOKs was assessed by real-time qPCR and ELISA. Results: An MOI of 1 was determined to be the appropriate ratio of bacteria and HOKs with substantial amounts of viable bacterial cells and HOKs in an antibiotic-free medium under aerobic conditions for 2 h. Sm and Pg significantly upregulated the expression of IL-6 and IL-8 (P < 0.05), while Ai and Aa could not induce significant levels of these cytokines with reference to the control. Conclusion: Within the limitations of this study, the current findings suggest that periodontal commensals and pathogens may differentially modulate immunoinflammatory response in human oral keratinocytes.
Persistent Identifierhttp://hdl.handle.net/10722/279964
ISSN
2015 SCImago Journal Rankings: 0.272

 

DC FieldValueLanguage
dc.contributor.authorLi, HJ-
dc.contributor.authorSeneviratne, CJ-
dc.contributor.authorWang, CY-
dc.contributor.authorJin, LJ-
dc.date.accessioned2019-12-23T08:24:19Z-
dc.date.available2019-12-23T08:24:19Z-
dc.date.issued2019-
dc.identifier.citationThe Chinese Journal of Dental Research, 2019, v. 22 n. 2, p. 105-112-
dc.identifier.issn1462-6446-
dc.identifier.urihttp://hdl.handle.net/10722/279964-
dc.description.abstractObjective: To investigate the immunoinflammatory response in the crosstalk of human oral keratinocytes (HOKs) with selected periodontal commensals and pathogens. Methods: Four representative viable oral bacteria, including periodontal commensals (Streptococcus mutans, Sm; and Actinomyces israelii, Ai) and pathogens (Aggregatibacter actinomycetemcomitans, Aa; and Porphyromonas gingivalis, Pg), were selected. A viable bacteria-HOKs interactive model was tested under various conditions of oxygen, antibiotics, duration and multiplicity of infection (MOI). The expression of IL-6 and IL-8 in HOKs was assessed by real-time qPCR and ELISA. Results: An MOI of 1 was determined to be the appropriate ratio of bacteria and HOKs with substantial amounts of viable bacterial cells and HOKs in an antibiotic-free medium under aerobic conditions for 2 h. Sm and Pg significantly upregulated the expression of IL-6 and IL-8 (P < 0.05), while Ai and Aa could not induce significant levels of these cytokines with reference to the control. Conclusion: Within the limitations of this study, the current findings suggest that periodontal commensals and pathogens may differentially modulate immunoinflammatory response in human oral keratinocytes.-
dc.languageeng-
dc.publisherQuintessence Publishing Co Ltd. The Journal's web site is located at http://www.quintpub.com/journals/cjdr/gp.php?journal_name=CJDR&name_abbr=CJDR-
dc.relation.ispartofThe Chinese Journal of Dental Research-
dc.subjectcommensals-
dc.subjectperiodontopathogens-
dc.subjecthuman oral keratinocytes-
dc.subjectbacteria-host interaction-
dc.subjectcytokines-
dc.titlePeriodontal commensals and pathogens differentially modulate immuno-inflammatory response in human oral keratinocytes-
dc.typeArticle-
dc.identifier.emailJin, LJ: ljjin@hkucc.hku.hk-
dc.identifier.authorityJin, LJ=rp00028-
dc.identifier.doi10.3290/j.cjdr.a42514-
dc.identifier.pmid31172138-
dc.identifier.hkuros308741-
dc.identifier.volume22-
dc.identifier.issue2-
dc.identifier.spage105-
dc.identifier.epage112-
dc.publisher.placeUnited Kingdom-

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