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Article: MicroRNA‐338‐5p reverses chemoresistance and inhibits invasion of esophageal squamous cell carcinoma cells by targeting Id‐1

TitleMicroRNA‐338‐5p reverses chemoresistance and inhibits invasion of esophageal squamous cell carcinoma cells by targeting Id‐1
Authors
Keywordschemoresistance
circulating miRNA
esophageal cancer
Id‐1
miR‐338‐5p
Issue Date2019
PublisherWiley Japan for Japanese Cancer Association. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=1347-9032&site=1
Citation
Cancer Science, 2019, v. 110 n. 12, p. 3677-3688 How to Cite?
Abstract5‐Fluorouracil (5‐FU) is a chemotherapeutic agent commonly used to treat esophageal squamous cell carcinoma (ESCC), but acquisition of chemoresistance frequently occurs and the underlying mechanisms are not fully understood. We found that microRNA (miR)‐338‐5p was underexpressed in ESCC cells with acquired 5‐FU chemoresistance. Forced expression of miR‐338‐5p in these cells resulted in downregulation of Id‐1, and restoration of both in vitro and in vivo sensitivity to 5‐FU treatment. The effects were abolished by reexpression of Id‐1. In contrast, miR‐338‐5p knockdown induced 5‐FU resistance in chemosensitive esophageal cell lines, and knockdown of both miR‐338‐5p and Id‐1 resensitized the cells to 5‐FU. In addition, miR‐338‐5p had suppressive effects on migration and invasion of ESCC cells. Luciferase reporter assay confirmed a direct interaction between miR‐338‐5p and the 3′‐UTR of Id‐1. We also found that miR‐338‐5p was significantly downregulated in tumor tissue and serum samples of patients with ESCC. Notably, low serum miR‐338‐5p expression level was associated with poorer survival and poor response to 5‐FU/cisplatin‐based neoadjuvant chemoradiotherapy. In summary, we found that miR‐338‐5p can modulate 5‐FU chemoresistance and inhibit invasion‐related functions in ESCC by negatively regulating Id‐1, and that serum miR‐338‐5p could be a novel noninvasive prognostic and predictive biomarker in ESCC.
Persistent Identifierhttp://hdl.handle.net/10722/279955
ISSN
2019 Impact Factor: 4.966
2015 SCImago Journal Rankings: 1.744
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorHan, L-
dc.contributor.authorCUI, D-
dc.contributor.authorLi, B-
dc.contributor.authorXu, WW-
dc.contributor.authorLam, AKY-
dc.contributor.authorChan, KT-
dc.contributor.authorZHU, Y-
dc.contributor.authorLee, NPY-
dc.contributor.authorLaw, SYK-
dc.contributor.authorGuan, XY-
dc.contributor.authorQin, YR-
dc.contributor.authorChan, KW-
dc.contributor.authorMa, S-
dc.contributor.authorTsao, SW-
dc.contributor.authorCheung, ALM-
dc.date.accessioned2019-12-23T08:24:11Z-
dc.date.available2019-12-23T08:24:11Z-
dc.date.issued2019-
dc.identifier.citationCancer Science, 2019, v. 110 n. 12, p. 3677-3688-
dc.identifier.issn1347-9032-
dc.identifier.urihttp://hdl.handle.net/10722/279955-
dc.description.abstract5‐Fluorouracil (5‐FU) is a chemotherapeutic agent commonly used to treat esophageal squamous cell carcinoma (ESCC), but acquisition of chemoresistance frequently occurs and the underlying mechanisms are not fully understood. We found that microRNA (miR)‐338‐5p was underexpressed in ESCC cells with acquired 5‐FU chemoresistance. Forced expression of miR‐338‐5p in these cells resulted in downregulation of Id‐1, and restoration of both in vitro and in vivo sensitivity to 5‐FU treatment. The effects were abolished by reexpression of Id‐1. In contrast, miR‐338‐5p knockdown induced 5‐FU resistance in chemosensitive esophageal cell lines, and knockdown of both miR‐338‐5p and Id‐1 resensitized the cells to 5‐FU. In addition, miR‐338‐5p had suppressive effects on migration and invasion of ESCC cells. Luciferase reporter assay confirmed a direct interaction between miR‐338‐5p and the 3′‐UTR of Id‐1. We also found that miR‐338‐5p was significantly downregulated in tumor tissue and serum samples of patients with ESCC. Notably, low serum miR‐338‐5p expression level was associated with poorer survival and poor response to 5‐FU/cisplatin‐based neoadjuvant chemoradiotherapy. In summary, we found that miR‐338‐5p can modulate 5‐FU chemoresistance and inhibit invasion‐related functions in ESCC by negatively regulating Id‐1, and that serum miR‐338‐5p could be a novel noninvasive prognostic and predictive biomarker in ESCC.-
dc.languageeng-
dc.publisherWiley Japan for Japanese Cancer Association. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=1347-9032&site=1-
dc.relation.ispartofCancer Science-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectchemoresistance-
dc.subjectcirculating miRNA-
dc.subjectesophageal cancer-
dc.subjectId‐1-
dc.subjectmiR‐338‐5p-
dc.titleMicroRNA‐338‐5p reverses chemoresistance and inhibits invasion of esophageal squamous cell carcinoma cells by targeting Id‐1-
dc.typeArticle-
dc.identifier.emailChan, KT: ktchan66@hku.hk-
dc.identifier.emailLee, NPY: nikkilee@hku.hk-
dc.identifier.emailLaw, SYK: slaw@hku.hk-
dc.identifier.emailGuan, XY: xyguan@hku.hk-
dc.identifier.emailChan, KW: kwchan@pathology.hku.hk-
dc.identifier.emailMa, S: stefma@hku.hk-
dc.identifier.emailTsao, SW: gswtsao@hku.hk-
dc.identifier.emailCheung, ALM: lmcheung@hku.hk-
dc.identifier.authorityLee, NPY=rp00263-
dc.identifier.authorityLaw, SYK=rp00437-
dc.identifier.authorityGuan, XY=rp00454-
dc.identifier.authorityChan, KW=rp00330-
dc.identifier.authorityMa, S=rp00506-
dc.identifier.authorityTsao, SW=rp00399-
dc.identifier.authorityCheung, ALM=rp00332-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1111/cas.14220-
dc.identifier.pmid31646712-
dc.identifier.pmcidPMC6890449-
dc.identifier.scopuseid_2-s2.0-85075155050-
dc.identifier.hkuros308838-
dc.identifier.volume110-
dc.identifier.issue12-
dc.identifier.spage3677-
dc.identifier.epage3688-
dc.publisher.placeJapan-

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