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- Publisher Website: 10.1016/j.bone.2018.08.015
- Scopus: eid_2-s2.0-85052523982
- PMID: 30149068
- WOS: WOS:000447232100032
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Article: Magnoflorine with hyaluronic acid gel promotes subchondral bone regeneration and attenuates cartilage degeneration in early osteoarthritis
Title | Magnoflorine with hyaluronic acid gel promotes subchondral bone regeneration and attenuates cartilage degeneration in early osteoarthritis |
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Authors | |
Keywords | Magnoflorine Osteoarthritis Articular cartilage Subchondral trabecular bone Hyaluronic acid gel |
Issue Date | 2018 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone |
Citation | Bone, 2018, v. 116, p. 266-278 How to Cite? |
Abstract | Objective:
To investigate efficacy of Chinese medicine magnoflorine combined with hyaluronic acid (HA)-gel in promoting subchondral bone (SCB) regeneration and attenuating cartilage degeneration in early osteoarthritis (OA).
Methods:
MC3T3-E1 under magnoflorine treatment was assayed by XTT to determine cell viability. Cell proliferation was reflected through cell cycle. Osteoblast mineralization was stained by Alizarin Red. Standardized bone canal of 1 mm in diameter and 4 mm in depth was made on tibial medial plateau of 4-month-old Dunkin-Hartley spontaneous knee OA guinea pigs. Guinea pigs (n = 5/group) were treated once intra-bone canal injection of 2 μl HA-gel, 2 μl HA-gel+50 ng magnoflorine and null (Defect) respectively, except sham group. The left hind limbs were harvested for μCT scan and histopathological staining 2-month post-surgery.
Results:
25 μg/ml magnoflorine treatment significantly increased cell viability, S-phase and mineralization of MC3T3-E1 cells. In vivo, HA-gel + magnoflorine treatment significantly altered SCB microstructure; changes included increase in bone volume fraction (BV/TV), trabecular number (Tb.N), connectivity density (Conn.Dn), and decrease in degree of anisotropy (DA), which implied trabecular bone regeneration. Treatment also resulted in a decrease in modified Mankin's scores, and an increase in volume ratio of hyaline cartilage (HC)/calcified cartilage (CC) and fractal dimension (FD, roughness indicator of osteochondral conjunction), compared to Defect and HA groups. Furthermore, FD was positively associated with volume ratio of HC/CC and negatively associated with modified Mankin's scores. Finally, histological results showed that due to a faster regeneration of SCB with the HA-gel + magnoflorine treatment, the reduction of cartilage matrix and the decreased expression of chondrogenic signals were attenuated.
Conclusion:
Our study elucidated the potential benefits of HA-gel + magnoflorine in promoting SCB regeneration and revealed a protective effect of stimulating recovery of the SCB integrity on attenuating cartilage degradation to prevent OA progression. |
Persistent Identifier | http://hdl.handle.net/10722/278235 |
ISSN | 2023 Impact Factor: 3.5 2023 SCImago Journal Rankings: 1.179 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Cai, Z | - |
dc.contributor.author | Feng, Y | - |
dc.contributor.author | LI, C | - |
dc.contributor.author | YANG, K | - |
dc.contributor.author | SUN, T | - |
dc.contributor.author | XU, L | - |
dc.contributor.author | CHEN, Y | - |
dc.contributor.author | Yan, CH | - |
dc.contributor.author | Lu, WW | - |
dc.contributor.author | Chiu, KY | - |
dc.date.accessioned | 2019-10-04T08:10:05Z | - |
dc.date.available | 2019-10-04T08:10:05Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Bone, 2018, v. 116, p. 266-278 | - |
dc.identifier.issn | 8756-3282 | - |
dc.identifier.uri | http://hdl.handle.net/10722/278235 | - |
dc.description.abstract | Objective: To investigate efficacy of Chinese medicine magnoflorine combined with hyaluronic acid (HA)-gel in promoting subchondral bone (SCB) regeneration and attenuating cartilage degeneration in early osteoarthritis (OA). Methods: MC3T3-E1 under magnoflorine treatment was assayed by XTT to determine cell viability. Cell proliferation was reflected through cell cycle. Osteoblast mineralization was stained by Alizarin Red. Standardized bone canal of 1 mm in diameter and 4 mm in depth was made on tibial medial plateau of 4-month-old Dunkin-Hartley spontaneous knee OA guinea pigs. Guinea pigs (n = 5/group) were treated once intra-bone canal injection of 2 μl HA-gel, 2 μl HA-gel+50 ng magnoflorine and null (Defect) respectively, except sham group. The left hind limbs were harvested for μCT scan and histopathological staining 2-month post-surgery. Results: 25 μg/ml magnoflorine treatment significantly increased cell viability, S-phase and mineralization of MC3T3-E1 cells. In vivo, HA-gel + magnoflorine treatment significantly altered SCB microstructure; changes included increase in bone volume fraction (BV/TV), trabecular number (Tb.N), connectivity density (Conn.Dn), and decrease in degree of anisotropy (DA), which implied trabecular bone regeneration. Treatment also resulted in a decrease in modified Mankin's scores, and an increase in volume ratio of hyaline cartilage (HC)/calcified cartilage (CC) and fractal dimension (FD, roughness indicator of osteochondral conjunction), compared to Defect and HA groups. Furthermore, FD was positively associated with volume ratio of HC/CC and negatively associated with modified Mankin's scores. Finally, histological results showed that due to a faster regeneration of SCB with the HA-gel + magnoflorine treatment, the reduction of cartilage matrix and the decreased expression of chondrogenic signals were attenuated. Conclusion: Our study elucidated the potential benefits of HA-gel + magnoflorine in promoting SCB regeneration and revealed a protective effect of stimulating recovery of the SCB integrity on attenuating cartilage degradation to prevent OA progression. | - |
dc.language | eng | - |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone | - |
dc.relation.ispartof | Bone | - |
dc.subject | Magnoflorine | - |
dc.subject | Osteoarthritis | - |
dc.subject | Articular cartilage | - |
dc.subject | Subchondral trabecular bone | - |
dc.subject | Hyaluronic acid gel | - |
dc.title | Magnoflorine with hyaluronic acid gel promotes subchondral bone regeneration and attenuates cartilage degeneration in early osteoarthritis | - |
dc.type | Article | - |
dc.identifier.email | Cai, Z: pdf95765@HKUCC-COM.hku.hk | - |
dc.identifier.email | Yan, CH: yanchoi@hku.hk | - |
dc.identifier.email | Lu, WW: wwlu@hku.hk | - |
dc.identifier.email | Chiu, KY: pkychiu@hkucc.hku.hk | - |
dc.identifier.authority | Yan, CH=rp00303 | - |
dc.identifier.authority | Lu, WW=rp00411 | - |
dc.identifier.authority | Chiu, KY=rp00379 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.bone.2018.08.015 | - |
dc.identifier.pmid | 30149068 | - |
dc.identifier.scopus | eid_2-s2.0-85052523982 | - |
dc.identifier.hkuros | 307060 | - |
dc.identifier.volume | 116 | - |
dc.identifier.spage | 266 | - |
dc.identifier.epage | 278 | - |
dc.identifier.isi | WOS:000447232100032 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1873-2763 | - |