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Article: Magnoflorine with hyaluronic acid gel promotes subchondral bone regeneration and attenuates cartilage degeneration in early osteoarthritis

TitleMagnoflorine with hyaluronic acid gel promotes subchondral bone regeneration and attenuates cartilage degeneration in early osteoarthritis
Authors
KeywordsMagnoflorine
Osteoarthritis
Articular cartilage
Subchondral trabecular bone
Hyaluronic acid gel
Issue Date2018
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone
Citation
Bone, 2018, v. 116, p. 266-278 How to Cite?
AbstractObjective: To investigate efficacy of Chinese medicine magnoflorine combined with hyaluronic acid (HA)-gel in promoting subchondral bone (SCB) regeneration and attenuating cartilage degeneration in early osteoarthritis (OA). Methods: MC3T3-E1 under magnoflorine treatment was assayed by XTT to determine cell viability. Cell proliferation was reflected through cell cycle. Osteoblast mineralization was stained by Alizarin Red. Standardized bone canal of 1 mm in diameter and 4 mm in depth was made on tibial medial plateau of 4-month-old Dunkin-Hartley spontaneous knee OA guinea pigs. Guinea pigs (n = 5/group) were treated once intra-bone canal injection of 2 μl HA-gel, 2 μl HA-gel+50 ng magnoflorine and null (Defect) respectively, except sham group. The left hind limbs were harvested for μCT scan and histopathological staining 2-month post-surgery. Results: 25 μg/ml magnoflorine treatment significantly increased cell viability, S-phase and mineralization of MC3T3-E1 cells. In vivo, HA-gel + magnoflorine treatment significantly altered SCB microstructure; changes included increase in bone volume fraction (BV/TV), trabecular number (Tb.N), connectivity density (Conn.Dn), and decrease in degree of anisotropy (DA), which implied trabecular bone regeneration. Treatment also resulted in a decrease in modified Mankin's scores, and an increase in volume ratio of hyaline cartilage (HC)/calcified cartilage (CC) and fractal dimension (FD, roughness indicator of osteochondral conjunction), compared to Defect and HA groups. Furthermore, FD was positively associated with volume ratio of HC/CC and negatively associated with modified Mankin's scores. Finally, histological results showed that due to a faster regeneration of SCB with the HA-gel + magnoflorine treatment, the reduction of cartilage matrix and the decreased expression of chondrogenic signals were attenuated. Conclusion: Our study elucidated the potential benefits of HA-gel + magnoflorine in promoting SCB regeneration and revealed a protective effect of stimulating recovery of the SCB integrity on attenuating cartilage degradation to prevent OA progression.
Persistent Identifierhttp://hdl.handle.net/10722/278235
ISSN
2019 Impact Factor: 4.147
2015 SCImago Journal Rankings: 1.752

 

DC FieldValueLanguage
dc.contributor.authorCai, Z-
dc.contributor.authorFeng, Y-
dc.contributor.authorLI, C-
dc.contributor.authorYANG, K-
dc.contributor.authorSUN, T-
dc.contributor.authorXU, L-
dc.contributor.authorCHEN, Y-
dc.contributor.authorYan, CH-
dc.contributor.authorLu, WW-
dc.contributor.authorChiu, KY-
dc.date.accessioned2019-10-04T08:10:05Z-
dc.date.available2019-10-04T08:10:05Z-
dc.date.issued2018-
dc.identifier.citationBone, 2018, v. 116, p. 266-278-
dc.identifier.issn8756-3282-
dc.identifier.urihttp://hdl.handle.net/10722/278235-
dc.description.abstractObjective: To investigate efficacy of Chinese medicine magnoflorine combined with hyaluronic acid (HA)-gel in promoting subchondral bone (SCB) regeneration and attenuating cartilage degeneration in early osteoarthritis (OA). Methods: MC3T3-E1 under magnoflorine treatment was assayed by XTT to determine cell viability. Cell proliferation was reflected through cell cycle. Osteoblast mineralization was stained by Alizarin Red. Standardized bone canal of 1 mm in diameter and 4 mm in depth was made on tibial medial plateau of 4-month-old Dunkin-Hartley spontaneous knee OA guinea pigs. Guinea pigs (n = 5/group) were treated once intra-bone canal injection of 2 μl HA-gel, 2 μl HA-gel+50 ng magnoflorine and null (Defect) respectively, except sham group. The left hind limbs were harvested for μCT scan and histopathological staining 2-month post-surgery. Results: 25 μg/ml magnoflorine treatment significantly increased cell viability, S-phase and mineralization of MC3T3-E1 cells. In vivo, HA-gel + magnoflorine treatment significantly altered SCB microstructure; changes included increase in bone volume fraction (BV/TV), trabecular number (Tb.N), connectivity density (Conn.Dn), and decrease in degree of anisotropy (DA), which implied trabecular bone regeneration. Treatment also resulted in a decrease in modified Mankin's scores, and an increase in volume ratio of hyaline cartilage (HC)/calcified cartilage (CC) and fractal dimension (FD, roughness indicator of osteochondral conjunction), compared to Defect and HA groups. Furthermore, FD was positively associated with volume ratio of HC/CC and negatively associated with modified Mankin's scores. Finally, histological results showed that due to a faster regeneration of SCB with the HA-gel + magnoflorine treatment, the reduction of cartilage matrix and the decreased expression of chondrogenic signals were attenuated. Conclusion: Our study elucidated the potential benefits of HA-gel + magnoflorine in promoting SCB regeneration and revealed a protective effect of stimulating recovery of the SCB integrity on attenuating cartilage degradation to prevent OA progression.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone-
dc.relation.ispartofBone-
dc.subjectMagnoflorine-
dc.subjectOsteoarthritis-
dc.subjectArticular cartilage-
dc.subjectSubchondral trabecular bone-
dc.subjectHyaluronic acid gel-
dc.titleMagnoflorine with hyaluronic acid gel promotes subchondral bone regeneration and attenuates cartilage degeneration in early osteoarthritis-
dc.typeArticle-
dc.identifier.emailCai, Z: pdf95765@HKUCC-COM.hku.hk-
dc.identifier.emailYan, CH: yanchoi@hku.hk-
dc.identifier.emailLu, WW: wwlu@hku.hk-
dc.identifier.emailChiu, KY: pkychiu@hkucc.hku.hk-
dc.identifier.authorityYan, CH=rp00303-
dc.identifier.authorityLu, WW=rp00411-
dc.identifier.authorityChiu, KY=rp00379-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bone.2018.08.015-
dc.identifier.pmid30149068-
dc.identifier.scopuseid_2-s2.0-85052523982-
dc.identifier.hkuros307060-
dc.identifier.volume116-
dc.identifier.spage266-
dc.identifier.epage278-
dc.publisher.placeUnited States-

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