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- Scopus: eid_2-s2.0-85072911348
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Article: Effect of a novel quaternary ammonium silane cavity disinfectant on cariogenic biofilm formation
Title | Effect of a novel quaternary ammonium silane cavity disinfectant on cariogenic biofilm formation |
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Authors | |
Keywords | Raman Dentine Quaternary ammonium silane Streptococcus mutans CFU |
Issue Date | 2020 |
Publisher | Springer for German Society of Oral and Maxillofacial Surgery. The Journal's web site is located at http://link.springer.de/link/service/journals/00784/index.htm |
Citation | Clinical Oral Investigations, 2020, v. 24, p. 649-661 How to Cite? |
Abstract | Objective:
Evaluate effect of quaternary ammonium silane (QAS) cavity disinfectant on cariogenic biofilm.
Materials and methods:
Single- (Streptococcus mutans or Lactobacillus acidophilus), dual- (Streptococcus mutans/Lactobacillus Acidophilus), and multi-species (Streptococcus mutans, Actinomyces naeslundii, and Streptococcus sanguis) biofilms were grown on acid-etched dentine discs. Biofilms were incubated (120 min/37 °C) and allowed to grow for 3 days anaerobically. Discs (no treatment) served as control (group 1). Groups II, III, IV, and V were then treated with 2% chlorhexidine, and 2%, 5%, and 10% QAS (20 s). Discs were returned to well plates with 300 μL of bacterial suspension and placed in anaerobic incubator at 37 °C and biofilms redeveloped for 4 days. Confocal microscopy, Raman, CFU, and MTT assay were performed.
Results:
Raman peaks show shifts at 1450 cm−1, 1453 cm−1, 1457 cm−1, 1460 cm−1, and 1462 cm−1 for control, 2% CHX, 2%, 5%, and 10% QAS groups in multi-species biofilms. There was reduction of 484 cm−1 band in 10% QAS group. CLSM revealed densely clustered green colonies in control group and red confluent QAS-treated biofilms with significantly lower log CFU for single/dual species. Metabolic activities of Streptococcus mutans and Lactobacillus acidophilus decreased with increasing QAS exposure time.
Conclusion:
Quaternary ammonium silanes possess antimicrobial activities and inhibit growth of cariogenic biofilms.
Clinical significance:
Available data demonstrated use of QAS as potential antibacterial cavity disinfectant in adhesive dentistry. Experimental QAS can effectively eliminate caries-forming bacteria, when used inside a prepared cavity, and can definitely overcome problems associated with present available cavity disinfectants. |
Persistent Identifier | http://hdl.handle.net/10722/277971 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.942 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Daood, U | - |
dc.contributor.author | Burrow, MF | - |
dc.contributor.author | Yiu, CKY | - |
dc.date.accessioned | 2019-10-04T08:04:55Z | - |
dc.date.available | 2019-10-04T08:04:55Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Clinical Oral Investigations, 2020, v. 24, p. 649-661 | - |
dc.identifier.issn | 1432-6981 | - |
dc.identifier.uri | http://hdl.handle.net/10722/277971 | - |
dc.description.abstract | Objective: Evaluate effect of quaternary ammonium silane (QAS) cavity disinfectant on cariogenic biofilm. Materials and methods: Single- (Streptococcus mutans or Lactobacillus acidophilus), dual- (Streptococcus mutans/Lactobacillus Acidophilus), and multi-species (Streptococcus mutans, Actinomyces naeslundii, and Streptococcus sanguis) biofilms were grown on acid-etched dentine discs. Biofilms were incubated (120 min/37 °C) and allowed to grow for 3 days anaerobically. Discs (no treatment) served as control (group 1). Groups II, III, IV, and V were then treated with 2% chlorhexidine, and 2%, 5%, and 10% QAS (20 s). Discs were returned to well plates with 300 μL of bacterial suspension and placed in anaerobic incubator at 37 °C and biofilms redeveloped for 4 days. Confocal microscopy, Raman, CFU, and MTT assay were performed. Results: Raman peaks show shifts at 1450 cm−1, 1453 cm−1, 1457 cm−1, 1460 cm−1, and 1462 cm−1 for control, 2% CHX, 2%, 5%, and 10% QAS groups in multi-species biofilms. There was reduction of 484 cm−1 band in 10% QAS group. CLSM revealed densely clustered green colonies in control group and red confluent QAS-treated biofilms with significantly lower log CFU for single/dual species. Metabolic activities of Streptococcus mutans and Lactobacillus acidophilus decreased with increasing QAS exposure time. Conclusion: Quaternary ammonium silanes possess antimicrobial activities and inhibit growth of cariogenic biofilms. Clinical significance: Available data demonstrated use of QAS as potential antibacterial cavity disinfectant in adhesive dentistry. Experimental QAS can effectively eliminate caries-forming bacteria, when used inside a prepared cavity, and can definitely overcome problems associated with present available cavity disinfectants. | - |
dc.language | eng | - |
dc.publisher | Springer for German Society of Oral and Maxillofacial Surgery. The Journal's web site is located at http://link.springer.de/link/service/journals/00784/index.htm | - |
dc.relation.ispartof | Clinical Oral Investigations | - |
dc.subject | Raman | - |
dc.subject | Dentine | - |
dc.subject | Quaternary ammonium silane | - |
dc.subject | Streptococcus mutans | - |
dc.subject | CFU | - |
dc.title | Effect of a novel quaternary ammonium silane cavity disinfectant on cariogenic biofilm formation | - |
dc.type | Article | - |
dc.identifier.email | Burrow, MF: mfburr58@hku.hk | - |
dc.identifier.email | Yiu, CKY: ckyyiu@hkucc.hku.hk | - |
dc.identifier.authority | Burrow, MF=rp01306 | - |
dc.identifier.authority | Yiu, CKY=rp00018 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00784-019-02928-7 | - |
dc.identifier.scopus | eid_2-s2.0-85072911348 | - |
dc.identifier.hkuros | 306951 | - |
dc.identifier.volume | 24 | - |
dc.identifier.spage | 649 | - |
dc.identifier.epage | 661 | - |
dc.identifier.isi | WOS:000520138900012 | - |
dc.publisher.place | Germany | - |
dc.identifier.issnl | 1432-6981 | - |