File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Image analysis of α/β-tubulin rings in two-dimensional crystalline arrays of periodic mesoporous nanostructures

TitleImage analysis of α/β-tubulin rings in two-dimensional crystalline arrays of periodic mesoporous nanostructures
Authors
Keywordsα/β-tubulin ring
Electron microscopy
Two-dimensional crystals
Issue Date2010
Citation
Journal of Biochemistry, 2010, v. 147, n. 4, p. 555-563 How to Cite?
AbstractTwo-dimensional crystalline arrays of periodic mesoporous nanostructures, consisting of α/β-tubulin ring-shaped oligomers in the double-layered architecture in an apolar arrangement are successfully prepared with PEM-G buffer containing Arginine nonamers and observed by electron microscopy. The ring-shaped α/β-tubulin assemblies are conformations related to a protofilament, although protofilaments are usually highly constrained in a straight conformation in microtubules. The two-dimensional crystalline arrays appear to have the structure of a square lattice with the layer group P4, the unit cell dimensions of a = b = 36.9 nm and γ = 90°. The isolated torus-like shaped projections of a pseudo-repeat structural unit are analysed with the method for single particle reconstruction. The average values of outer- and inner-diameters for the torus-like shapes are 49.0 ± 1.0, and 25.4 ± 1.1 nm, respectively. The thickness of the peripheral masses consisting of two concentric sub-rings is 11.8 ± 1.0 nm, where α/β- tubulin heterodimers appear to reside alternately. Taking the average sizes of the rings and the crystallographic unit cell dimensions into consideration, the discussion is focused on the most probable arrangement of the α/β-tubulin rings in the two-dimensional crystalline arrays. © 2009 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/276858
ISSN
2023 Impact Factor: 2.1
2023 SCImago Journal Rankings: 0.687
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorIshii, Noriyuki-
dc.contributor.authorOkuro, Kou-
dc.contributor.authorKinbara, Kazushi-
dc.contributor.authorAida, Takuzo-
dc.date.accessioned2019-09-18T08:34:52Z-
dc.date.available2019-09-18T08:34:52Z-
dc.date.issued2010-
dc.identifier.citationJournal of Biochemistry, 2010, v. 147, n. 4, p. 555-563-
dc.identifier.issn0021-924X-
dc.identifier.urihttp://hdl.handle.net/10722/276858-
dc.description.abstractTwo-dimensional crystalline arrays of periodic mesoporous nanostructures, consisting of α/β-tubulin ring-shaped oligomers in the double-layered architecture in an apolar arrangement are successfully prepared with PEM-G buffer containing Arginine nonamers and observed by electron microscopy. The ring-shaped α/β-tubulin assemblies are conformations related to a protofilament, although protofilaments are usually highly constrained in a straight conformation in microtubules. The two-dimensional crystalline arrays appear to have the structure of a square lattice with the layer group P4, the unit cell dimensions of a = b = 36.9 nm and γ = 90°. The isolated torus-like shaped projections of a pseudo-repeat structural unit are analysed with the method for single particle reconstruction. The average values of outer- and inner-diameters for the torus-like shapes are 49.0 ± 1.0, and 25.4 ± 1.1 nm, respectively. The thickness of the peripheral masses consisting of two concentric sub-rings is 11.8 ± 1.0 nm, where α/β- tubulin heterodimers appear to reside alternately. Taking the average sizes of the rings and the crystallographic unit cell dimensions into consideration, the discussion is focused on the most probable arrangement of the α/β-tubulin rings in the two-dimensional crystalline arrays. © 2009 The Authors.-
dc.languageeng-
dc.relation.ispartofJournal of Biochemistry-
dc.subjectα/β-tubulin ring-
dc.subjectElectron microscopy-
dc.subjectTwo-dimensional crystals-
dc.titleImage analysis of α/β-tubulin rings in two-dimensional crystalline arrays of periodic mesoporous nanostructures-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/jb/mvp201-
dc.identifier.pmid19959504-
dc.identifier.scopuseid_2-s2.0-77950598561-
dc.identifier.volume147-
dc.identifier.issue4-
dc.identifier.spage555-
dc.identifier.epage563-
dc.identifier.eissn1756-2651-
dc.identifier.isiWOS:000276304200012-
dc.identifier.issnl0021-924X-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats