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- Publisher Website: 10.1128/JVI.01559-16
- Scopus: eid_2-s2.0-85001073400
- PMID: 27654286
- WOS: WOS:000389904500017
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Article: The Glycosylphosphatidylinositol-Anchored Variable Region of Llama Heavy Chain-Only Antibody JM4 Efficiently Blocks both Cell-Free and T Cell-T Cell Transmission of Human Immunodeficiency Virus Type 1
Title | The Glycosylphosphatidylinositol-Anchored Variable Region of Llama Heavy Chain-Only Antibody JM4 Efficiently Blocks both Cell-Free and T Cell-T Cell Transmission of Human Immunodeficiency Virus Type 1 |
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Authors | |
Issue Date | 2016 |
Publisher | American Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/ |
Citation | Journal of Virology, 2016, v. 90 n. 23, p. 10642-10659 How to Cite? |
Abstract | Lipid rafts are specialized dynamic microdomains of the plasma membrane and have been shown to be gateways for HIV-1 budding as well as entry into T cells and macrophages. In nature, many glycosylphosphatidylinositol (GPI)-anchored proteins localize in the lipid rafts. In the present study, we developed GPI-anchored variable regions (VHHs) of two heavy chain-only antibodies, JM2 and JM4, from immunized llamas. We show that by genetically linking the VHHs with a GPI attachment signal, VHHs are targeted to the lipid rafts of the plasma membranes. GPI-VHH JM4, but not GPI-VHH JM2, in transduced CD4(+) cell lines and human primary CD4 T cells not only efficiently blocks diverse HIV-1 strains, including tier 2 or 3 strains, transmitted founders, quasispecies, and soluble sdAb JM4-resistant strains, but also efficiently interferes T cell-T cell transmissions of HIV-1 and HIV-1 envelope-mediated fusion. Our findings should have important implications in GPI-anchored antibody-based therapy against HIV-1. |
Persistent Identifier | http://hdl.handle.net/10722/273972 |
ISSN | 2023 Impact Factor: 4.0 2023 SCImago Journal Rankings: 1.378 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Liu, L | - |
dc.contributor.author | Wang, W | - |
dc.contributor.author | Matz, J | - |
dc.contributor.author | Ye, C | - |
dc.contributor.author | Bracq, L | - |
dc.contributor.author | Delon, J | - |
dc.contributor.author | Kimata, JT | - |
dc.contributor.author | Chen, Z | - |
dc.contributor.author | Benichou, S | - |
dc.contributor.author | Zhou, P | - |
dc.date.accessioned | 2019-08-18T14:52:28Z | - |
dc.date.available | 2019-08-18T14:52:28Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Journal of Virology, 2016, v. 90 n. 23, p. 10642-10659 | - |
dc.identifier.issn | 0022-538X | - |
dc.identifier.uri | http://hdl.handle.net/10722/273972 | - |
dc.description.abstract | Lipid rafts are specialized dynamic microdomains of the plasma membrane and have been shown to be gateways for HIV-1 budding as well as entry into T cells and macrophages. In nature, many glycosylphosphatidylinositol (GPI)-anchored proteins localize in the lipid rafts. In the present study, we developed GPI-anchored variable regions (VHHs) of two heavy chain-only antibodies, JM2 and JM4, from immunized llamas. We show that by genetically linking the VHHs with a GPI attachment signal, VHHs are targeted to the lipid rafts of the plasma membranes. GPI-VHH JM4, but not GPI-VHH JM2, in transduced CD4(+) cell lines and human primary CD4 T cells not only efficiently blocks diverse HIV-1 strains, including tier 2 or 3 strains, transmitted founders, quasispecies, and soluble sdAb JM4-resistant strains, but also efficiently interferes T cell-T cell transmissions of HIV-1 and HIV-1 envelope-mediated fusion. Our findings should have important implications in GPI-anchored antibody-based therapy against HIV-1. | - |
dc.language | eng | - |
dc.publisher | American Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/ | - |
dc.relation.ispartof | Journal of Virology | - |
dc.rights | Journal of Virology. Copyright © American Society for Microbiology. | - |
dc.title | The Glycosylphosphatidylinositol-Anchored Variable Region of Llama Heavy Chain-Only Antibody JM4 Efficiently Blocks both Cell-Free and T Cell-T Cell Transmission of Human Immunodeficiency Virus Type 1 | - |
dc.type | Article | - |
dc.identifier.email | Chen, Z: zchenai@hku.hk | - |
dc.identifier.authority | Chen, Z=rp00243 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1128/JVI.01559-16 | - |
dc.identifier.pmid | 27654286 | - |
dc.identifier.scopus | eid_2-s2.0-85001073400 | - |
dc.identifier.hkuros | 301447 | - |
dc.identifier.volume | 90 | - |
dc.identifier.issue | 23 | - |
dc.identifier.spage | 10642 | - |
dc.identifier.epage | 10659 | - |
dc.identifier.isi | WOS:000389904500017 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-538X | - |