Identification and characterization of the interactome of core Planar cell polarity (PCP) protein Vangl2

Abstract

Planar cell polarity (PCP) is a major type of cell polarity, which regulates coordinated polarized cell behaviors during tissue morphogenesis and organogenesis. Disruption of PCP pathway leads to a variety of severe human diseases, ranging from neural tube defects, skeletal dysplasias, epilepsy to cancer metastasis. The asymmetric localized core PCP proteins such as Vangl2 is essential for the establishment of PCP. However, the downstream effectors of these core PCP proteins that help to regulate cell behaviors remain to be characterized. In this study, we utilized BioID system to identify novel interacting partners of Vangl2. Among all identified candidates, we found that Mark3 can interact with and might phosphorylate Vangl2 at its C terminus. This phosphorylation can increase Vangl2’s interaction with Scrib, a key apical-basal polarity protein, and such interaction is important for convergent extension process and synaptogenesis.