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Conference Paper: Specific flavonoid B-ring hydroxylases (CYP75B members) are recruited for different biosynthesis pathways of flavone-derived metabolites in grass biomass
Title | Specific flavonoid B-ring hydroxylases (CYP75B members) are recruited for different biosynthesis pathways of flavone-derived metabolites in grass biomass |
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Authors | |
Issue Date | 2019 |
Citation | 6th Plant Genomics & Gene Editing Congress: Asia co-located with Microbiome for Agriculture Congress Asia 2019, Kuala Lumpur, Malaysia, 29-30 July 2019 How to Cite? |
Abstract | Members in the cytochrome P750 75B subfamily are hydroxylases that modify the B-ring substitution patterns in flavonoids, impacting their structure and functions. In rice biomass, OsF2H and OsFNSII function as key enzymes directing flavanones to separate biosynthetic routes for extractable flavone C-glycosides and tricin-derived metabolites (soluble and lignin-bound), respectively. Here, CYP75B3 and CYP75B4 showed co-expression pattern with OsF2H and OsFNSII, respectively, in vegetative tissues. CRISPR/Cas-9 homozygous mutants and T-DNA insertion lines were generated (or obtained) to investigate their flavonoid profile, cell wall structure, and biomass digestibility. Accordingly, we established CYP75B3 as the sole F3´H in flavone C-glycosides biosynthesis and CYP75B4 as an indispensable 3´,5´-hydroxylase to produce soluble tricin derivative and tricin-bound lignin. Hence, CYP75B3 and CYP75B4 represent two different pathway-specific enzymes co-evolved with OsF2H and OsFNSII, respectively. Interestingly, the OsF2H-CYP75B3 and OsFNSII-CYP75B4 pairs are highly conserved in the grass family. Furthermore, CYP75B4 disruption resulted in reduced lignin content, altered S/G ratio, and enhanced enzymatic saccharification efficiency in biomass. Therefore, genetic manipulation of tricin biosynthesis presents a novel approach to engineer grass lignin for improved biomass utilization. |
Persistent Identifier | http://hdl.handle.net/10722/272556 |
DC Field | Value | Language |
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dc.contributor.author | Lo, CSC | - |
dc.date.accessioned | 2019-07-20T10:44:34Z | - |
dc.date.available | 2019-07-20T10:44:34Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | 6th Plant Genomics & Gene Editing Congress: Asia co-located with Microbiome for Agriculture Congress Asia 2019, Kuala Lumpur, Malaysia, 29-30 July 2019 | - |
dc.identifier.uri | http://hdl.handle.net/10722/272556 | - |
dc.description.abstract | Members in the cytochrome P750 75B subfamily are hydroxylases that modify the B-ring substitution patterns in flavonoids, impacting their structure and functions. In rice biomass, OsF2H and OsFNSII function as key enzymes directing flavanones to separate biosynthetic routes for extractable flavone C-glycosides and tricin-derived metabolites (soluble and lignin-bound), respectively. Here, CYP75B3 and CYP75B4 showed co-expression pattern with OsF2H and OsFNSII, respectively, in vegetative tissues. CRISPR/Cas-9 homozygous mutants and T-DNA insertion lines were generated (or obtained) to investigate their flavonoid profile, cell wall structure, and biomass digestibility. Accordingly, we established CYP75B3 as the sole F3´H in flavone C-glycosides biosynthesis and CYP75B4 as an indispensable 3´,5´-hydroxylase to produce soluble tricin derivative and tricin-bound lignin. Hence, CYP75B3 and CYP75B4 represent two different pathway-specific enzymes co-evolved with OsF2H and OsFNSII, respectively. Interestingly, the OsF2H-CYP75B3 and OsFNSII-CYP75B4 pairs are highly conserved in the grass family. Furthermore, CYP75B4 disruption resulted in reduced lignin content, altered S/G ratio, and enhanced enzymatic saccharification efficiency in biomass. Therefore, genetic manipulation of tricin biosynthesis presents a novel approach to engineer grass lignin for improved biomass utilization. | - |
dc.language | eng | - |
dc.relation.ispartof | 6th Plant Genomics & Gene Editing Congress: Asia, 2019 | - |
dc.title | Specific flavonoid B-ring hydroxylases (CYP75B members) are recruited for different biosynthesis pathways of flavone-derived metabolites in grass biomass | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Lo, CSC: clivelo@hku.hk | - |
dc.identifier.authority | Lo, CSC=rp00751 | - |
dc.identifier.hkuros | 299210 | - |