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Article: Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury

TitleSingle-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
Authors
KeywordsHeart
Pericardium
Epicardial cells
Issue Date2019
PublishereLife Sciences Publications Ltd. The Journal's web site is located at http://elifesciences.org/
Citation
eLife, 2019, v. 8, p. e43882 How to Cite?
AbstractBesides cardiomyocytes (CM), the heart contains numerous interstitial cell types which play key roles in heart repair, regeneration and disease, including fibroblast, vascular and immune cells. However, a comprehensive understanding of this interactive cell community is lacking. We performed single-cell RNA-sequencing of the total non-CM fraction and enriched (Pdgfra-GFP+) fibroblast lineage cells from murine hearts at days 3 and 7 post-sham or myocardial infarction (MI) surgery. Clustering of >30,000 single cells identified >30 populations representing nine cell lineages, including a previously undescribed fibroblast lineage trajectory present in both sham and MI hearts leading to a uniquely activated cell state defined in part by a strong anti-WNT transcriptome signature. We also uncovered novel myofibroblast subtypes expressing either pro-fibrotic or anti-fibrotic signatures. Our data highlight non-linear dynamics in myeloid and fibroblast lineages after cardiac injury, and provide an entry point for deeper analysis of cardiac homeostasis, inflammation, fibrosis, repair and regeneration.
Persistent Identifierhttp://hdl.handle.net/10722/271189
ISSN
2023 Impact Factor: 6.4
2023 SCImago Journal Rankings: 3.932
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFarbehi, N-
dc.contributor.authorPatrick, R-
dc.contributor.authorDorison, A-
dc.contributor.authorXaymardan, M-
dc.contributor.authorJanbandhu, V-
dc.contributor.authorWystub-Lis, K-
dc.contributor.authorHo, JWK-
dc.contributor.authorNordon, RE-
dc.contributor.authorHarvey, RP-
dc.date.accessioned2019-06-24T01:05:05Z-
dc.date.available2019-06-24T01:05:05Z-
dc.date.issued2019-
dc.identifier.citationeLife, 2019, v. 8, p. e43882-
dc.identifier.issn2050-084X-
dc.identifier.urihttp://hdl.handle.net/10722/271189-
dc.description.abstractBesides cardiomyocytes (CM), the heart contains numerous interstitial cell types which play key roles in heart repair, regeneration and disease, including fibroblast, vascular and immune cells. However, a comprehensive understanding of this interactive cell community is lacking. We performed single-cell RNA-sequencing of the total non-CM fraction and enriched (Pdgfra-GFP+) fibroblast lineage cells from murine hearts at days 3 and 7 post-sham or myocardial infarction (MI) surgery. Clustering of >30,000 single cells identified >30 populations representing nine cell lineages, including a previously undescribed fibroblast lineage trajectory present in both sham and MI hearts leading to a uniquely activated cell state defined in part by a strong anti-WNT transcriptome signature. We also uncovered novel myofibroblast subtypes expressing either pro-fibrotic or anti-fibrotic signatures. Our data highlight non-linear dynamics in myeloid and fibroblast lineages after cardiac injury, and provide an entry point for deeper analysis of cardiac homeostasis, inflammation, fibrosis, repair and regeneration.-
dc.languageeng-
dc.publishereLife Sciences Publications Ltd. The Journal's web site is located at http://elifesciences.org/-
dc.relation.ispartofeLife-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectHeart-
dc.subjectPericardium-
dc.subjectEpicardial cells-
dc.titleSingle-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury-
dc.typeArticle-
dc.identifier.emailHo, JWK: jwkho@hku.hk-
dc.identifier.authorityHo, JWK=rp02436-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.7554/eLife.43882-
dc.identifier.pmid30912746-
dc.identifier.pmcidPMC6459677-
dc.identifier.scopuseid_2-s2.0-85064723182-
dc.identifier.hkuros298177-
dc.identifier.volume8-
dc.identifier.spagee43882-
dc.identifier.epagee43882-
dc.identifier.isiWOS:000464312200001-
dc.publisher.placeCambridge, UK-
dc.identifier.issnl2050-084X-

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