Dopamine Luminescent Detection in Biological Matrix by a Copper(II) Complex

Abstract

Molecule specific luminescent probes have emerged as efficient tools for the detection and imaging of various important species in different biological matrix. A main challenge in the development of analyte-specific fluorescent probe is to identify an efficient recognition strategy that selectively recognizes and reports the presence of the targeted bioanalyte in a competitive biological environment. In this presentation, a biomimetic reaction-based strategy for developing selective fluorescent turn-on probes for the detection of dopamine will be discussed. Inspired by dopamine β-hydroxylase, our dopamine detection features the use of a copper(II) complex for an oxidative release of a fluorescent reporter. The dopamine probes developed are highly selective and sensitive, and can be applied in different complex matrix including human plasma, live cells and brain tissue. The same strategy is also extended to other biologically important redox species including superoxide and ascorbate. REFERENCES [1] Yu Z. H., Chung C. Y.-S., Tang F. K., Brewer T. F. and Au-Yeung H. Y., 'A Modular Trigger for the Development of Selective Superoxide Probes', Chem. Commun. 2017, 53, 10042-10045 [2] Au-Yeung H. Y., Chan C. Y., Tong K. Y., Yu Z. H., 'Copper-based Reactions in Analyte-Responsive Fluorescent Probes for Biological Applications', J. Inorg. Biochem. 2017, 177, 300-312