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Article: The Wheat Lr67 Gene from the Sugar Transport Protein 13 Family Confers Multipathogen Resistance in Barley

TitleThe Wheat Lr67 Gene from the Sugar Transport Protein 13 Family Confers Multipathogen Resistance in Barley
Authors
Issue Date2019
PublisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org
Citation
Plant Physiology, 2019, v. 179 n. 4, p. 1285-1297 How to Cite?
AbstractFungal pathogens are a major constraint to global crop production; hence, plant genes encoding pathogen resistance are important tools for combating disease. A few resistance genes identified to date provide partial, durable resistance to multiple pathogens and the wheat (Triticum aestivum) Lr67 hexose transporter variant (Lr67res) fits into this category. Two amino acids differ between the wild-type and resistant alleles – G144R and V387L. Exome sequence data from 267 barley (Hordeum vulgare) landraces and wild accessions was screened and neither of the Lr67res mutations was detected. The barley ortholog of Lr67, HvSTP13, was functionally characterized in yeast as a high affinity hexose transporter. The G144R mutation was introduced into HvSTP13 and abolished Glc uptake, whereas the V387L mutation reduced Glc uptake by ∼ 50%. Glc transport by HvSTP13 heterologously expressed in yeast was reduced when coexpressed with Lr67res. Stable transgenic Lr67res barley lines exhibited seedling resistance to the barley-specific pathogens Puccinia hordei and Blumeria graminis f. sp. hordei, which cause leaf rust and powdery mildew, respectively. Barley plants expressing Lr67res exhibited early senescence and higher pathogenesis-related (PR) gene expression. Unlike previous observations implicating flavonoids in the resistance of transgenic sorghum (Sorghum bicolor) expressing Lr34res, another wheat multipathogen resistance gene, barley flavonoids are unlikely to have a role in Lr67res-mediated resistance. Similar to observations made in yeast, Lr67res reduced Glc uptake in planta. These results confirm that the pathway by which Lr67res confers resistance to fungal pathogens is conserved between wheat and barley.
Persistent Identifierhttp://hdl.handle.net/10722/269517
ISSN
2023 Impact Factor: 6.5
2023 SCImago Journal Rankings: 2.101
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMilne, RJ-
dc.contributor.authorDibley, KE-
dc.contributor.authorSchnippenkoetter, W-
dc.contributor.authorMascher, M-
dc.contributor.authorLUI, ACW-
dc.contributor.authorWANG, L-
dc.contributor.authorLo, C-
dc.contributor.authorAshton, AR-
dc.contributor.authorRyan, PR-
dc.contributor.authorLagudah, ES-
dc.date.accessioned2019-04-24T08:09:20Z-
dc.date.available2019-04-24T08:09:20Z-
dc.date.issued2019-
dc.identifier.citationPlant Physiology, 2019, v. 179 n. 4, p. 1285-1297-
dc.identifier.issn0032-0889-
dc.identifier.urihttp://hdl.handle.net/10722/269517-
dc.description.abstractFungal pathogens are a major constraint to global crop production; hence, plant genes encoding pathogen resistance are important tools for combating disease. A few resistance genes identified to date provide partial, durable resistance to multiple pathogens and the wheat (Triticum aestivum) Lr67 hexose transporter variant (Lr67res) fits into this category. Two amino acids differ between the wild-type and resistant alleles – G144R and V387L. Exome sequence data from 267 barley (Hordeum vulgare) landraces and wild accessions was screened and neither of the Lr67res mutations was detected. The barley ortholog of Lr67, HvSTP13, was functionally characterized in yeast as a high affinity hexose transporter. The G144R mutation was introduced into HvSTP13 and abolished Glc uptake, whereas the V387L mutation reduced Glc uptake by ∼ 50%. Glc transport by HvSTP13 heterologously expressed in yeast was reduced when coexpressed with Lr67res. Stable transgenic Lr67res barley lines exhibited seedling resistance to the barley-specific pathogens Puccinia hordei and Blumeria graminis f. sp. hordei, which cause leaf rust and powdery mildew, respectively. Barley plants expressing Lr67res exhibited early senescence and higher pathogenesis-related (PR) gene expression. Unlike previous observations implicating flavonoids in the resistance of transgenic sorghum (Sorghum bicolor) expressing Lr34res, another wheat multipathogen resistance gene, barley flavonoids are unlikely to have a role in Lr67res-mediated resistance. Similar to observations made in yeast, Lr67res reduced Glc uptake in planta. These results confirm that the pathway by which Lr67res confers resistance to fungal pathogens is conserved between wheat and barley.-
dc.languageeng-
dc.publisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org-
dc.relation.ispartofPlant Physiology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleThe Wheat Lr67 Gene from the Sugar Transport Protein 13 Family Confers Multipathogen Resistance in Barley-
dc.typeArticle-
dc.identifier.emailLo, C: clivelo@hku.hk-
dc.identifier.authorityLo, C=rp00751-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1104/pp.18.00945-
dc.identifier.pmid30305371-
dc.identifier.pmcidPMC6446772-
dc.identifier.scopuseid_2-s2.0-85060910371-
dc.identifier.hkuros297365-
dc.identifier.volume179-
dc.identifier.issue4-
dc.identifier.spage1285-
dc.identifier.epage1297-
dc.identifier.isiWOS:000462993100010-
dc.publisher.placeUnited States-
dc.identifier.f1000734212456-
dc.identifier.issnl0032-0889-

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