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Article: Life-long in vivo cell-lineage tracing shows that no oogenesis originates from putative germline stem cells in adult mice

TitleLife-long in vivo cell-lineage tracing shows that no oogenesis originates from putative germline stem cells in adult mice
Authors
KeywordsFollicle tracing
Oocyte tracing
Life-long observations
Genetically modified mouse models
No postnatal oocyte regeneration
Issue Date2014
Citation
Proceedings of the National Academy of Sciences of the United States of America, 2014, v. 111, n. 50, p. 17983-17988 How to Cite?
AbstractWhether or not oocyte regeneration occurs in adult life has been the subject of much debate. In this study, we have traced germcell lineages over the life spans of three genetically modified mouse models and provide direct evidence that oogenesis does not originate from any germline stem cells (GSCs) in adult mice. By selective ablation of all existing oocytes in a Gdf9-Cre;iDTR mouse model, we have demonstrated that no new germ cells were ever regenerated under pathological conditions. By in vivo tracing of oocytes and follicles in the Sohlh1-CreERT2;R26R and Foxl2-CreERT2;mT/mG mouse models, respectively, we have shown that the initial pool of oocytes is the only source of germ cells throughout the life span of the mice and that no adult oogenesis ever occurs under physiological conditions. Our findings clearly show that there are no GSCs that contribute to adult oogenesis in mice and that the initial pool of oocytes formed in early life is the only source of germ cells throughout the entire reproductive life span.
Persistent Identifierhttp://hdl.handle.net/10722/265495
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, Hua-
dc.contributor.authorLiu, Lian-
dc.contributor.authorLi, Xin-
dc.contributor.authorBusayavalasa, Kiran-
dc.contributor.authorShen, Yan-
dc.contributor.authorHovatta, Outi-
dc.contributor.authorGustafsson, Jan Åke-
dc.contributor.authorLiu, Kui-
dc.date.accessioned2018-12-03T01:20:50Z-
dc.date.available2018-12-03T01:20:50Z-
dc.date.issued2014-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2014, v. 111, n. 50, p. 17983-17988-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/265495-
dc.description.abstractWhether or not oocyte regeneration occurs in adult life has been the subject of much debate. In this study, we have traced germcell lineages over the life spans of three genetically modified mouse models and provide direct evidence that oogenesis does not originate from any germline stem cells (GSCs) in adult mice. By selective ablation of all existing oocytes in a Gdf9-Cre;iDTR mouse model, we have demonstrated that no new germ cells were ever regenerated under pathological conditions. By in vivo tracing of oocytes and follicles in the Sohlh1-CreERT2;R26R and Foxl2-CreERT2;mT/mG mouse models, respectively, we have shown that the initial pool of oocytes is the only source of germ cells throughout the life span of the mice and that no adult oogenesis ever occurs under physiological conditions. Our findings clearly show that there are no GSCs that contribute to adult oogenesis in mice and that the initial pool of oocytes formed in early life is the only source of germ cells throughout the entire reproductive life span.-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.subjectFollicle tracing-
dc.subjectOocyte tracing-
dc.subjectLife-long observations-
dc.subjectGenetically modified mouse models-
dc.subjectNo postnatal oocyte regeneration-
dc.titleLife-long in vivo cell-lineage tracing shows that no oogenesis originates from putative germline stem cells in adult mice-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1073/pnas.1421047111-
dc.identifier.pmid25453063-
dc.identifier.scopuseid_2-s2.0-84919401701-
dc.identifier.volume111-
dc.identifier.issue50-
dc.identifier.spage17983-
dc.identifier.epage17988-
dc.identifier.eissn1091-6490-
dc.identifier.isiWOS:000357160100002-
dc.identifier.f1000725255586-
dc.identifier.issnl0027-8424-

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