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Article: Rapid endosomal escape of prickly nanodiamonds: Implications for gene delivery

TitleRapid endosomal escape of prickly nanodiamonds: Implications for gene delivery
Authors
Issue Date2015
Citation
Scientific Reports, 2015, v. 5 How to Cite?
AbstractThe prickly nanodiamonds easily entered cells via endocytosis followed by unique intracellular translocation characteristics - quick endosomal escape followed by stable residence in cytoplasm. Endosomal membrane rupturing is identified as the major route of nanodiamonds' escaping the vesicle confinement and to the cytoplasm. Little cytotoxicity is observed to associate with the nanodiamonds' cytosolic release. Such features enable its application for gene delivery, which requires both effective cellular uptake and cytosolic release of the gene. Taking green fluorescent protein gene as an example, we demonstrate the successful cytosolic delivery and expression of such a gene using the prickly nanodiamonds as carrier.
Persistent Identifierhttp://hdl.handle.net/10722/265434
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChu, Zhiqin-
dc.contributor.authorMiu, Kaikei-
dc.contributor.authorLung, Pingsai-
dc.contributor.authorZhang, Silu-
dc.contributor.authorZhao, Saisai-
dc.contributor.authorChang, Huan Cheng-
dc.contributor.authorLin, Ge-
dc.contributor.authorLi, Quan-
dc.date.accessioned2018-12-03T01:20:39Z-
dc.date.available2018-12-03T01:20:39Z-
dc.date.issued2015-
dc.identifier.citationScientific Reports, 2015, v. 5-
dc.identifier.urihttp://hdl.handle.net/10722/265434-
dc.description.abstractThe prickly nanodiamonds easily entered cells via endocytosis followed by unique intracellular translocation characteristics - quick endosomal escape followed by stable residence in cytoplasm. Endosomal membrane rupturing is identified as the major route of nanodiamonds' escaping the vesicle confinement and to the cytoplasm. Little cytotoxicity is observed to associate with the nanodiamonds' cytosolic release. Such features enable its application for gene delivery, which requires both effective cellular uptake and cytosolic release of the gene. Taking green fluorescent protein gene as an example, we demonstrate the successful cytosolic delivery and expression of such a gene using the prickly nanodiamonds as carrier.-
dc.languageeng-
dc.relation.ispartofScientific Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleRapid endosomal escape of prickly nanodiamonds: Implications for gene delivery-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/srep11661-
dc.identifier.pmid26123532-
dc.identifier.scopuseid_2-s2.0-84934784026-
dc.identifier.volume5-
dc.identifier.spagenull-
dc.identifier.epagenull-
dc.identifier.eissn2045-2322-
dc.identifier.isiWOS:000357104500001-
dc.identifier.issnl2045-2322-

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