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Article: LncRNA PVT1 triggers Cyto-protective autophagy and promotes pancreatic ductal adenocarcinoma development via the miR-20a-5p/ULK1 Axis
Title | LncRNA PVT1 triggers Cyto-protective autophagy and promotes pancreatic ductal adenocarcinoma development via the miR-20a-5p/ULK1 Axis |
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Authors | |
Keywords | Autophagy Long non-coding RNA MiR-20a-5p Pancreatic ductal adenocarcinoma PVT1 ULK1 |
Issue Date | 2018 |
Publisher | BioMed Central Ltd. The Journal's web site is located at http://www.molecular-cancer.com |
Citation | Molecular Cancer, 2018, v. 17, p. 98 How to Cite? |
Abstract | BACKGROUND: Defective autophagy is thought to contribute to the pathogenesis of many diseases, including cancer. Human plasmacytoma variant translocation 1 (PVT1) is an oncogenic long non-coding RNA that has been identified as a prognostic biomarker in pancreatic ductal adenocarcinoma, but how PVT1 operates in the regulation of autophagy in pancreatic ductal adenocarcinoma (PDA) is unclear. METHODS: PVT1 expression level was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and hybridization in situ (ISH). Western blot or qRT-PCR was performed to assess the ULK1 protein or mRNA level. Autophagy was explored via autophagic flux detection under a confocal microscope and autophagic vacuoles investigation under a transmission electron microscopy (TEM). The biological role of PVT1 in autophagy and PDA development was determined by gain-of-function and loss-of-function assays. RESULTS: We found that PVT1 levels paralleled those of ULK1 protein in PDA cancer tissues. PVT1 promoted cyto-protective autophagy and cell growth by targeting ULK1 both in vitro and in vivo. Moreover, high PVT1 expression was associated with poor prognosis. Furthermore, we found that PVT1 acted as sponge to regulate miR-20a-5p and thus affected ULK1 expression and the development of pancreatic ductal adenocarcinoma. CONCLUSIONS: The present study demonstrates that the 'PVT1/miR-20a-5p/ULK1/autophagy' pathway modulates the development of pancreatic ductal adenocarcinoma and may be a novel target for developing therapeutic strategies for pancreatic ductal adenocarcinoma. |
Persistent Identifier | http://hdl.handle.net/10722/262369 |
ISSN | 2023 Impact Factor: 27.7 2023 SCImago Journal Rankings: 8.222 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Huang, F | - |
dc.contributor.author | Chen, W | - |
dc.contributor.author | Peng, J | - |
dc.contributor.author | Li, Y | - |
dc.contributor.author | Zhuang, Y | - |
dc.contributor.author | Zhu, Z | - |
dc.contributor.author | Shao, C | - |
dc.contributor.author | Yang, W | - |
dc.contributor.author | Yao, H | - |
dc.contributor.author | Zhang, S | - |
dc.date.accessioned | 2018-09-28T04:58:09Z | - |
dc.date.available | 2018-09-28T04:58:09Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Molecular Cancer, 2018, v. 17, p. 98 | - |
dc.identifier.issn | 1476-4598 | - |
dc.identifier.uri | http://hdl.handle.net/10722/262369 | - |
dc.description.abstract | BACKGROUND: Defective autophagy is thought to contribute to the pathogenesis of many diseases, including cancer. Human plasmacytoma variant translocation 1 (PVT1) is an oncogenic long non-coding RNA that has been identified as a prognostic biomarker in pancreatic ductal adenocarcinoma, but how PVT1 operates in the regulation of autophagy in pancreatic ductal adenocarcinoma (PDA) is unclear. METHODS: PVT1 expression level was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and hybridization in situ (ISH). Western blot or qRT-PCR was performed to assess the ULK1 protein or mRNA level. Autophagy was explored via autophagic flux detection under a confocal microscope and autophagic vacuoles investigation under a transmission electron microscopy (TEM). The biological role of PVT1 in autophagy and PDA development was determined by gain-of-function and loss-of-function assays. RESULTS: We found that PVT1 levels paralleled those of ULK1 protein in PDA cancer tissues. PVT1 promoted cyto-protective autophagy and cell growth by targeting ULK1 both in vitro and in vivo. Moreover, high PVT1 expression was associated with poor prognosis. Furthermore, we found that PVT1 acted as sponge to regulate miR-20a-5p and thus affected ULK1 expression and the development of pancreatic ductal adenocarcinoma. CONCLUSIONS: The present study demonstrates that the 'PVT1/miR-20a-5p/ULK1/autophagy' pathway modulates the development of pancreatic ductal adenocarcinoma and may be a novel target for developing therapeutic strategies for pancreatic ductal adenocarcinoma. | - |
dc.language | eng | - |
dc.publisher | BioMed Central Ltd. The Journal's web site is located at http://www.molecular-cancer.com | - |
dc.relation.ispartof | Molecular Cancer | - |
dc.rights | Molecular Cancer. Copyright © BioMed Central Ltd. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Autophagy | - |
dc.subject | Long non-coding RNA | - |
dc.subject | MiR-20a-5p | - |
dc.subject | Pancreatic ductal adenocarcinoma | - |
dc.subject | PVT1 | - |
dc.subject | ULK1 | - |
dc.title | LncRNA PVT1 triggers Cyto-protective autophagy and promotes pancreatic ductal adenocarcinoma development via the miR-20a-5p/ULK1 Axis | - |
dc.type | Article | - |
dc.identifier.email | Yang, W: yangwl@hku.hk | - |
dc.identifier.authority | Yang, W=rp00524 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1186/s12943-018-0845-6 | - |
dc.identifier.scopus | eid_2-s2.0-85050016845 | - |
dc.identifier.hkuros | 292099 | - |
dc.identifier.volume | 17 | - |
dc.identifier.spage | 98 | - |
dc.identifier.epage | 98 | - |
dc.identifier.isi | WOS:000438503700001 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 1476-4598 | - |