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Article: Effective differentiation of induced pluripotent stem cells into dental cells

TitleEffective differentiation of induced pluripotent stem cells into dental cells
Authors
Keywordsbiotooth
iPSCs
recombinations
transwell
dental epithelial cells
Issue Date2019
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38417
Citation
Developmental Dynamics, 2019, v. 248 n. 1, p. 129-139 How to Cite?
AbstractBackground: A biotooth is defined as a complete living tooth, made in laboratory cultures from a spontaneous interplay between epithelial and mesenchymal cell‐based frontal systems. A good solution to these problems is to use induced pluripotent stem cells (iPSCs). However, no one has yet formulated culture conditions that effectively differentiate iPSCs into dental epithelial and dental mesenchymal cells phenotypes analogous to those present in tooth development. Results: Here, we tried to induce differentiation methods for dental epithelial cells (DEC) and dental mesenchymal cells from iPSCs. For the DEC differentiation, the conditional media of SF2 DEC was adjusted to embryoid body. Moreover, we now report on a new cultivation protocol, supported by transwell membrane cell culture that make it possible to differentiate iPSCs into dental epithelial and mesenchymal cells with abilities to initiate the first stages in de novo tooth formation. Conclusions: Implementation of technical modifications to the protocol that maximize the number and rate of iPSC differentiation, into mesenchymal and epithelial cell layers, will be the next step toward growing an anatomically accurate biomimetic tooth organ. Developmental Dynamics 248:129–139, 2019. © 2018 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/261357
ISSN
2019 Impact Factor: 3.275
2015 SCImago Journal Rankings: 1.726
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKim, EJ-
dc.contributor.authorYoon, KS-
dc.contributor.authorArakaki, M-
dc.contributor.authorOtsu, K-
dc.contributor.authorFukumoto, S-
dc.contributor.authorHarada, H-
dc.contributor.authorGreen, DW-
dc.contributor.authorLee, JM-
dc.contributor.authorJung, HS-
dc.date.accessioned2018-09-14T08:56:50Z-
dc.date.available2018-09-14T08:56:50Z-
dc.date.issued2019-
dc.identifier.citationDevelopmental Dynamics, 2019, v. 248 n. 1, p. 129-139-
dc.identifier.issn1058-8388-
dc.identifier.urihttp://hdl.handle.net/10722/261357-
dc.description.abstractBackground: A biotooth is defined as a complete living tooth, made in laboratory cultures from a spontaneous interplay between epithelial and mesenchymal cell‐based frontal systems. A good solution to these problems is to use induced pluripotent stem cells (iPSCs). However, no one has yet formulated culture conditions that effectively differentiate iPSCs into dental epithelial and dental mesenchymal cells phenotypes analogous to those present in tooth development. Results: Here, we tried to induce differentiation methods for dental epithelial cells (DEC) and dental mesenchymal cells from iPSCs. For the DEC differentiation, the conditional media of SF2 DEC was adjusted to embryoid body. Moreover, we now report on a new cultivation protocol, supported by transwell membrane cell culture that make it possible to differentiate iPSCs into dental epithelial and mesenchymal cells with abilities to initiate the first stages in de novo tooth formation. Conclusions: Implementation of technical modifications to the protocol that maximize the number and rate of iPSC differentiation, into mesenchymal and epithelial cell layers, will be the next step toward growing an anatomically accurate biomimetic tooth organ. Developmental Dynamics 248:129–139, 2019. © 2018 Wiley Periodicals, Inc.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38417-
dc.relation.ispartofDevelopmental Dynamics-
dc.rightsPostprint This is the peer reviewed version of the following article: [Developmental Dynamics, 2019, v. 248 n. 1, p. 129-139], which has been published in final form at [http://dx.doi.org/10.1002/dvdy.24663]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectbiotooth-
dc.subjectiPSCs-
dc.subjectrecombinations-
dc.subjecttranswell-
dc.subjectdental epithelial cells-
dc.titleEffective differentiation of induced pluripotent stem cells into dental cells-
dc.typeArticle-
dc.identifier.emailJung, HS: hsjung@hku.hk-
dc.identifier.authorityJung, HS=rp01683-
dc.description.naturepostprint-
dc.identifier.doi10.1002/dvdy.24663-
dc.identifier.pmid30106495-
dc.identifier.scopuseid_2-s2.0-85054292468-
dc.identifier.hkuros291084-
dc.identifier.volume248-
dc.identifier.issue1-
dc.identifier.spage129-
dc.identifier.epage139-
dc.identifier.isiWOS:000454597700012-
dc.publisher.placeUnited States-
dc.identifier.issnl1058-8388-

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