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Conference Paper: Effective gene silencing effect mediated by the KL4/siRNA complexes in lung epithelial cells through clathrin-dependent endocytosis
| Title | Effective gene silencing effect mediated by the KL4/siRNA complexes in lung epithelial cells through clathrin-dependent endocytosis |
|---|---|
| Authors | |
| Issue Date | 2017 |
| Publisher | The Aerosol Society. |
| Citation | Drug Delivery to the Lungs Conference (DDL), Edinburgh, UK, 6-8 December 2017 How to Cite? |
| Abstract | Small interfering RNA (siRNA) have been widely investigated for the treatment of various respiratory diseases. A vector with good safety and high efficacy for pulmonary siRNA delivery is a major problem that needs to be addressed. The synthetic peptide KL4 is a 21-residue peptide containing hydrophobic leucine interspersed with cationic lysine, which was designed to mimic the function of surfactant protein B (SP-B). Previous result showed that the KL4/siRNA complexes can successfully induce gene silencing in lung epithelial cells. The aim of this study was to further study the uptake profile of the KL4/siRNA complexes, in order to optimize the system for pulmonary delivery. The result showed that clathrin-mediated endocytosis was involved in cellular uptake of KL4/siRNA complexes. Future study will focus on the in vivo transfection through intratracheal delivery of the complexes. |
| Description | Poster presentation |
| Persistent Identifier | http://hdl.handle.net/10722/258163 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Qiu, Y | - |
| dc.contributor.author | Chow, YT | - |
| dc.contributor.author | Lam, JKW | - |
| dc.date.accessioned | 2018-08-22T01:33:59Z | - |
| dc.date.available | 2018-08-22T01:33:59Z | - |
| dc.date.issued | 2017 | - |
| dc.identifier.citation | Drug Delivery to the Lungs Conference (DDL), Edinburgh, UK, 6-8 December 2017 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/258163 | - |
| dc.description | Poster presentation | - |
| dc.description.abstract | Small interfering RNA (siRNA) have been widely investigated for the treatment of various respiratory diseases. A vector with good safety and high efficacy for pulmonary siRNA delivery is a major problem that needs to be addressed. The synthetic peptide KL4 is a 21-residue peptide containing hydrophobic leucine interspersed with cationic lysine, which was designed to mimic the function of surfactant protein B (SP-B). Previous result showed that the KL4/siRNA complexes can successfully induce gene silencing in lung epithelial cells. The aim of this study was to further study the uptake profile of the KL4/siRNA complexes, in order to optimize the system for pulmonary delivery. The result showed that clathrin-mediated endocytosis was involved in cellular uptake of KL4/siRNA complexes. Future study will focus on the in vivo transfection through intratracheal delivery of the complexes. | - |
| dc.language | eng | - |
| dc.publisher | The Aerosol Society. | - |
| dc.relation.ispartof | Drug Delivery to the Lungs Conference (DDL) 2017 | - |
| dc.title | Effective gene silencing effect mediated by the KL4/siRNA complexes in lung epithelial cells through clathrin-dependent endocytosis | - |
| dc.type | Conference_Paper | - |
| dc.identifier.email | Chow, YT: mytchow@hku.hk | - |
| dc.identifier.email | Lam, JKW: jkwlam@hku.hk | - |
| dc.identifier.authority | Lam, JKW=rp01346 | - |
| dc.identifier.hkuros | 286666 | - |