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Article: Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments

TitleGradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments
Authors
KeywordsProtein engineering
Non-canonical amino acids
DMD (digital micromirror device)
Protein patterning
Protein lithography
Protein gradient
Issue Date2009
Citation
Biomedical Microdevices, 2009, v. 11, n. 5, p. 1127-1134 How to Cite?
AbstractSpatial patterning of proteins is a valuable technique for many biological applications and is the prevailing tool for defining microenvironments for cells in culture, a required procedure in developmental biology and tissue engineering research. However, it is still challenging to achieve protein patterns that closely mimic native microenvironments, such as gradient protein distributions with desirable mechanical properties. By combining projection dynamic mask lithography and protein engineering with non-canonical photosensitive amino acids, we demonstrate a simple, scalable strategy to fabricate any user-defined 2D or 3D stable gradient pattern with complex geometries from an artificial extracellular matrix (aECM) protein. We show that the elastic modulus and chemical nature of the gradient profile are biocompatible and allow useful applications in cell biological research. © 2009 Springer Science+Business Media, LLC.
Persistent Identifierhttp://hdl.handle.net/10722/257313
ISSN
2023 Impact Factor: 3.0
2023 SCImago Journal Rankings: 0.578
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Sheng-
dc.contributor.authorWong Po Foo, Cheryl-
dc.contributor.authorWarrier, Ajithkumar-
dc.contributor.authorPoo, Mu Ming-
dc.contributor.authorHeilshorn, Sarah C.-
dc.contributor.authorZhang, Xiang-
dc.date.accessioned2018-07-24T08:59:26Z-
dc.date.available2018-07-24T08:59:26Z-
dc.date.issued2009-
dc.identifier.citationBiomedical Microdevices, 2009, v. 11, n. 5, p. 1127-1134-
dc.identifier.issn1387-2176-
dc.identifier.urihttp://hdl.handle.net/10722/257313-
dc.description.abstractSpatial patterning of proteins is a valuable technique for many biological applications and is the prevailing tool for defining microenvironments for cells in culture, a required procedure in developmental biology and tissue engineering research. However, it is still challenging to achieve protein patterns that closely mimic native microenvironments, such as gradient protein distributions with desirable mechanical properties. By combining projection dynamic mask lithography and protein engineering with non-canonical photosensitive amino acids, we demonstrate a simple, scalable strategy to fabricate any user-defined 2D or 3D stable gradient pattern with complex geometries from an artificial extracellular matrix (aECM) protein. We show that the elastic modulus and chemical nature of the gradient profile are biocompatible and allow useful applications in cell biological research. © 2009 Springer Science+Business Media, LLC.-
dc.languageeng-
dc.relation.ispartofBiomedical Microdevices-
dc.subjectProtein engineering-
dc.subjectNon-canonical amino acids-
dc.subjectDMD (digital micromirror device)-
dc.subjectProtein patterning-
dc.subjectProtein lithography-
dc.subjectProtein gradient-
dc.titleGradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10544-009-9329-1-
dc.identifier.pmid19495986-
dc.identifier.scopuseid_2-s2.0-70549092045-
dc.identifier.volume11-
dc.identifier.issue5-
dc.identifier.spage1127-
dc.identifier.epage1134-
dc.identifier.isiWOS:000270679400019-
dc.identifier.issnl1387-2176-

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