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- Publisher Website: 10.1242/jcs.075366
- Scopus: eid_2-s2.0-78349244870
- PMID: 20980394
- WOS: WOS:000283798600012
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Article: Cdc14p resets the competency of replication licensing by dephosphorylating multiple initiation proteins during mitotic exit in budding yeast
Title | Cdc14p resets the competency of replication licensing by dephosphorylating multiple initiation proteins during mitotic exit in budding yeast |
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Authors | |
Keywords | Cdc14p phosphatase Cell cycle DNA replication Replication licensing Replication-initiation proteins Budding yeast |
Issue Date | 2010 |
Publisher | The Company of Biologists Ltd. The Journal's web site is located at https://jcs.biologists.org/ |
Citation | Journal of Cell Science, 2010, v. 123, n. 22, p. 3933-3943 How to Cite? |
Abstract | In eukaryotes, replication licensing is achieved through sequential loading of several replication-initiation proteins onto replication origins to form pre-replicative complexes (pre-RCs), and unscheduled replication licensing is prevented by cyclin-dependent kinases (CDKs) through inhibitory phosphorylations of multiple initiation proteins. It is known that CDK inactivation during mitotic exit promotes pre-RC formation for the next cell cycle. However, whether the removal of the inhibitory phosphorylations on the initiation proteins is essential and the identity of the acting phosphatase(s) remain unknown. Here, we show that cell division cycle protein 14 (Cdc14p) dephosphorylates replication-initiation proteins Orc2p, Orc6p, Cdc6p and Mcm3p to restore their competence for pre-RC assembly in the budding yeast Saccharomyces cerevisiae. Cells without functional Cdc14p fail to dephosphorylate initiation proteins and to form pre-RCs - even when CDK activities are suppressed - and cannot replicate DNA in mitotic rereplication systems, whereas pulsed ectopic expression of Cdc14p in mitotic cells results in efficient pre-RC assembly and DNA rereplication. Furthermore, Cdc14p becomes dispensable for DNA rereplication in mitotic cells with combined non-phosphorylatable and/or phosphorylation- insensitive alleles of the initiation proteins. These data unravel the essential role of Cdc14p in replication licensing, beyond its established functions in mitotic exit, providing new insight into the intricate regulation of DNA replication through the interplay of CDKs and the Cdc14p phosphatase. |
Persistent Identifier | http://hdl.handle.net/10722/256018 |
ISSN | 2023 Impact Factor: 3.3 2023 SCImago Journal Rankings: 1.587 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhai, Yuanliang | - |
dc.contributor.author | Yung, Philip Y.K. | - |
dc.contributor.author | Huo, Lin | - |
dc.contributor.author | Liang, Chun | - |
dc.date.accessioned | 2018-07-16T06:14:21Z | - |
dc.date.available | 2018-07-16T06:14:21Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Journal of Cell Science, 2010, v. 123, n. 22, p. 3933-3943 | - |
dc.identifier.issn | 0021-9533 | - |
dc.identifier.uri | http://hdl.handle.net/10722/256018 | - |
dc.description.abstract | In eukaryotes, replication licensing is achieved through sequential loading of several replication-initiation proteins onto replication origins to form pre-replicative complexes (pre-RCs), and unscheduled replication licensing is prevented by cyclin-dependent kinases (CDKs) through inhibitory phosphorylations of multiple initiation proteins. It is known that CDK inactivation during mitotic exit promotes pre-RC formation for the next cell cycle. However, whether the removal of the inhibitory phosphorylations on the initiation proteins is essential and the identity of the acting phosphatase(s) remain unknown. Here, we show that cell division cycle protein 14 (Cdc14p) dephosphorylates replication-initiation proteins Orc2p, Orc6p, Cdc6p and Mcm3p to restore their competence for pre-RC assembly in the budding yeast Saccharomyces cerevisiae. Cells without functional Cdc14p fail to dephosphorylate initiation proteins and to form pre-RCs - even when CDK activities are suppressed - and cannot replicate DNA in mitotic rereplication systems, whereas pulsed ectopic expression of Cdc14p in mitotic cells results in efficient pre-RC assembly and DNA rereplication. Furthermore, Cdc14p becomes dispensable for DNA rereplication in mitotic cells with combined non-phosphorylatable and/or phosphorylation- insensitive alleles of the initiation proteins. These data unravel the essential role of Cdc14p in replication licensing, beyond its established functions in mitotic exit, providing new insight into the intricate regulation of DNA replication through the interplay of CDKs and the Cdc14p phosphatase. | - |
dc.language | eng | - |
dc.publisher | The Company of Biologists Ltd. The Journal's web site is located at https://jcs.biologists.org/ | - |
dc.relation.ispartof | Journal of Cell Science | - |
dc.subject | Cdc14p phosphatase | - |
dc.subject | Cell cycle | - |
dc.subject | DNA replication | - |
dc.subject | Replication licensing | - |
dc.subject | Replication-initiation proteins | - |
dc.subject | Budding yeast | - |
dc.title | Cdc14p resets the competency of replication licensing by dephosphorylating multiple initiation proteins during mitotic exit in budding yeast | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1242/jcs.075366 | - |
dc.identifier.pmid | 20980394 | - |
dc.identifier.scopus | eid_2-s2.0-78349244870 | - |
dc.identifier.volume | 123 | - |
dc.identifier.issue | 22 | - |
dc.identifier.spage | 3933 | - |
dc.identifier.epage | 3943 | - |
dc.identifier.isi | WOS:000283798600012 | - |
dc.identifier.issnl | 0021-9533 | - |