File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1002/pmic.200800185
- Scopus: eid_2-s2.0-59449102709
- PMID: 19053083
- WOS: WOS:000262568500005
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Activity profiling of platelets by chemical proteomics
Title | Activity profiling of platelets by chemical proteomics |
---|---|
Authors | |
Keywords | Platelets Label-free quantification Chemical proteomics Nucleo-tides Affinity chromatography |
Issue Date | 2009 |
Citation | Proteomics, 2009, v. 9, n. 1, p. 40-50 How to Cite? |
Abstract | Chemical proteomics or activity based proteomics is a functional proteomics technology where molecular probes are used to target a selective group of functionally related proteins. Its emergence has enabled specific targeting of subproteomes, overcoming the limitations in dynamic range of traditional large-scale proteomics experiments. Using a chemical proteomics strategy, we attempt to differentially profile the nucleotide-binding proteome of active and resting platelets. We apply an affinity chromatography protocol using immobilized adenosine triphosphate, cyclic adenosine monophosphate, and cyclic guanosine monophosphate. The specificity of the immobilized nucleotides was demonstrated by competitive assays and by immunoblotting. LC coupled MS/MS was applied to identify the proteins recovered by our chemical proteomics strategy. When compared to a standard set of platelet lysate proteins, we confirmed that enrichment for nucleotide-binding proteins was indeed taking place. Finally, by employing label-free MS-based comparative quantification, we found a small number of platelet proteins that show statistically significant difference between the active and resting nucleotide-binding proteome. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Persistent Identifier | http://hdl.handle.net/10722/250921 |
ISSN | 2023 Impact Factor: 3.4 2023 SCImago Journal Rankings: 1.011 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, Jason W H | - |
dc.contributor.author | McRedmond, James P. | - |
dc.contributor.author | Cagney, Gerard | - |
dc.date.accessioned | 2018-02-01T01:54:05Z | - |
dc.date.available | 2018-02-01T01:54:05Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Proteomics, 2009, v. 9, n. 1, p. 40-50 | - |
dc.identifier.issn | 1615-9853 | - |
dc.identifier.uri | http://hdl.handle.net/10722/250921 | - |
dc.description.abstract | Chemical proteomics or activity based proteomics is a functional proteomics technology where molecular probes are used to target a selective group of functionally related proteins. Its emergence has enabled specific targeting of subproteomes, overcoming the limitations in dynamic range of traditional large-scale proteomics experiments. Using a chemical proteomics strategy, we attempt to differentially profile the nucleotide-binding proteome of active and resting platelets. We apply an affinity chromatography protocol using immobilized adenosine triphosphate, cyclic adenosine monophosphate, and cyclic guanosine monophosphate. The specificity of the immobilized nucleotides was demonstrated by competitive assays and by immunoblotting. LC coupled MS/MS was applied to identify the proteins recovered by our chemical proteomics strategy. When compared to a standard set of platelet lysate proteins, we confirmed that enrichment for nucleotide-binding proteins was indeed taking place. Finally, by employing label-free MS-based comparative quantification, we found a small number of platelet proteins that show statistically significant difference between the active and resting nucleotide-binding proteome. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | - |
dc.language | eng | - |
dc.relation.ispartof | Proteomics | - |
dc.subject | Platelets | - |
dc.subject | Label-free quantification | - |
dc.subject | Chemical proteomics | - |
dc.subject | Nucleo-tides | - |
dc.subject | Affinity chromatography | - |
dc.title | Activity profiling of platelets by chemical proteomics | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/pmic.200800185 | - |
dc.identifier.pmid | 19053083 | - |
dc.identifier.scopus | eid_2-s2.0-59449102709 | - |
dc.identifier.volume | 9 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 40 | - |
dc.identifier.epage | 50 | - |
dc.identifier.eissn | 1615-9861 | - |
dc.identifier.isi | WOS:000262568500005 | - |
dc.identifier.issnl | 1615-9853 | - |