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Conference Paper: A dox-terminable cell-encapsulating device for intravitreal drug delivery
Title | A dox-terminable cell-encapsulating device for intravitreal drug delivery |
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Authors | |
Issue Date | 2016 |
Citation | 28th Annual Scientific Meeting of Hong Kong Ophthalmological Symposium: Ophthalmic Diagnostics and Lasers, Hong Kong, 19-20 November 2016 How to Cite? |
Abstract | Objectives: Encapsulated-cell therapy presents a way of localized and sustained drug delivery to the posterior eye. An injectable collagen-alginate drug delivery platform was designed with promising result previously. Now, a Tet-On inducible apoptotic control is further introduced for safety.
Methods: GDNF-expression HEK293 cells were transfected with pcDNATM6/TR and recombinant Caspase 8 plasmid. Cell death upon Dox exposure in cell-encapsulating collagen-alginate gel was assayed by MTS. Biocompatibility and stability of the gel system as well as its encapsulated cell viability were evaluated on 7 and 28 days post intravitreal injection by microscopy.
Results & conclusion: Complete cell death via caspase-mediated apoptosis was achievable within 72 hours of 0-2ug/ml Dox induction. Intravitreally injected device displayed good biocompatibility, mechanical stability and encapsulated cell viability after prolonged implantation in RCS rat eyes.
We have successfully established a Dox-terminable cell-encapsulating intravitreal drug delivery device with good biocompatibility and stability after implantation in rats. |
Description | The Symposium was jointly organized by the Hong Kong Ophthalmological Society and the College of Ophthalmologists of Hong Kong Poster Presentation |
Persistent Identifier | http://hdl.handle.net/10722/249513 |
DC Field | Value | Language |
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dc.contributor.author | Tsang, KK | - |
dc.contributor.author | Wong, FSY | - |
dc.contributor.author | Yao, KM | - |
dc.contributor.author | Chan, BP | - |
dc.contributor.author | Lai, JSM | - |
dc.contributor.author | Shih, KC | - |
dc.contributor.author | Lo, ACY | - |
dc.date.accessioned | 2017-11-21T03:03:17Z | - |
dc.date.available | 2017-11-21T03:03:17Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | 28th Annual Scientific Meeting of Hong Kong Ophthalmological Symposium: Ophthalmic Diagnostics and Lasers, Hong Kong, 19-20 November 2016 | - |
dc.identifier.uri | http://hdl.handle.net/10722/249513 | - |
dc.description | The Symposium was jointly organized by the Hong Kong Ophthalmological Society and the College of Ophthalmologists of Hong Kong | - |
dc.description | Poster Presentation | - |
dc.description.abstract | Objectives: Encapsulated-cell therapy presents a way of localized and sustained drug delivery to the posterior eye. An injectable collagen-alginate drug delivery platform was designed with promising result previously. Now, a Tet-On inducible apoptotic control is further introduced for safety. Methods: GDNF-expression HEK293 cells were transfected with pcDNATM6/TR and recombinant Caspase 8 plasmid. Cell death upon Dox exposure in cell-encapsulating collagen-alginate gel was assayed by MTS. Biocompatibility and stability of the gel system as well as its encapsulated cell viability were evaluated on 7 and 28 days post intravitreal injection by microscopy. Results & conclusion: Complete cell death via caspase-mediated apoptosis was achievable within 72 hours of 0-2ug/ml Dox induction. Intravitreally injected device displayed good biocompatibility, mechanical stability and encapsulated cell viability after prolonged implantation in RCS rat eyes. We have successfully established a Dox-terminable cell-encapsulating intravitreal drug delivery device with good biocompatibility and stability after implantation in rats. | - |
dc.language | eng | - |
dc.relation.ispartof | Hong Kong Ophthalmological Symposium 2016 | - |
dc.title | A dox-terminable cell-encapsulating device for intravitreal drug delivery | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Wong, FSY: frann@hku.hk | - |
dc.identifier.email | Yao, KM: kmyao@hku.hk | - |
dc.identifier.email | Chan, BP: bpchan@hku.hk | - |
dc.identifier.email | Lai, JSM: laism@hku.hk | - |
dc.identifier.email | Shih, KC: kcshih@hku.hk | - |
dc.identifier.email | Lo, ACY: amylo@hku.hk | - |
dc.identifier.authority | Yao, KM=rp00344 | - |
dc.identifier.authority | Chan, BP=rp00087 | - |
dc.identifier.authority | Lai, JSM=rp00295 | - |
dc.identifier.authority | Shih, KC=rp01374 | - |
dc.identifier.authority | Lo, ACY=rp00425 | - |
dc.identifier.hkuros | 282657 | - |