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Conference Paper: Anti-autophagic effects of lutein in retinal ischemia
| Title | Anti-autophagic effects of lutein in retinal ischemia |
|---|---|
| Authors | |
| Issue Date | 2016 |
| Citation | 28th Annual Scientific Meeting of Hong Kong Ophthalmological Symposium: Ophthalmic Diagnostics and Lasers, Hong Kong, 19-20 November 2016 How to Cite? |
| Abstract | Objective: Lutein protects the retina from ischemia/reperfusion (I/R) injuries through anti-oxidative, anti-apoptotic and anti-inflammatory properties. The role of lutein and autophagy in I/R-mediated retinal cell death is unknown. We further investigated the anti-autophagic effects of lutein-mediated protection in in vitro and in vivo models.
Method: Cultured rat Müller cells (rMC-1) were exposed to cobalt(II) chloride (CoCl2) with or without lutein treatment. Cell viability (MTS assay), cell death (LDH assay), autophagy (Western blot of microtubule-associated protein light chain 3 LC3) and autophagic vesicle formation (immunohistochemistry) were assessed. Mice with middle cerebral artery occlusion-induced retinal I/R injury received lutein or vehicle treatment. Immunohistochemical detection of LC3 was performed on retinal sections.
Results: Lutein treatment was able to improve cell viability, reduce LDH release and protect rMC-1 cells from CoCl2-induced autophagic cell death. It can also reduce I/R-induced autophagic activity in the retina. |
| Description | The Symposium was jointly organized by the Hong Kong Ophthalmological Society and the College of Ophthalmologists of Hong Kong Free Paper - Vitreoretina |
| Persistent Identifier | http://hdl.handle.net/10722/249502 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Tang, LHC | - |
| dc.contributor.author | Lo, ACY | - |
| dc.contributor.author | Fung, FKC | - |
| dc.date.accessioned | 2017-11-21T03:03:08Z | - |
| dc.date.available | 2017-11-21T03:03:08Z | - |
| dc.date.issued | 2016 | - |
| dc.identifier.citation | 28th Annual Scientific Meeting of Hong Kong Ophthalmological Symposium: Ophthalmic Diagnostics and Lasers, Hong Kong, 19-20 November 2016 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/249502 | - |
| dc.description | The Symposium was jointly organized by the Hong Kong Ophthalmological Society and the College of Ophthalmologists of Hong Kong | - |
| dc.description | Free Paper - Vitreoretina | - |
| dc.description.abstract | Objective: Lutein protects the retina from ischemia/reperfusion (I/R) injuries through anti-oxidative, anti-apoptotic and anti-inflammatory properties. The role of lutein and autophagy in I/R-mediated retinal cell death is unknown. We further investigated the anti-autophagic effects of lutein-mediated protection in in vitro and in vivo models. Method: Cultured rat Müller cells (rMC-1) were exposed to cobalt(II) chloride (CoCl2) with or without lutein treatment. Cell viability (MTS assay), cell death (LDH assay), autophagy (Western blot of microtubule-associated protein light chain 3 LC3) and autophagic vesicle formation (immunohistochemistry) were assessed. Mice with middle cerebral artery occlusion-induced retinal I/R injury received lutein or vehicle treatment. Immunohistochemical detection of LC3 was performed on retinal sections. Results: Lutein treatment was able to improve cell viability, reduce LDH release and protect rMC-1 cells from CoCl2-induced autophagic cell death. It can also reduce I/R-induced autophagic activity in the retina. | - |
| dc.language | eng | - |
| dc.relation.ispartof | Hong Kong Ophthalmological Symposium 2016 | - |
| dc.title | Anti-autophagic effects of lutein in retinal ischemia | - |
| dc.type | Conference_Paper | - |
| dc.identifier.email | Fung, FKC: frederic@hkucc.hku.hk | - |
| dc.identifier.email | Lo, ACY: amylo@hku.hk | - |
| dc.identifier.authority | Lo, ACY=rp00425 | - |
| dc.identifier.hkuros | 282639 | - |