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Article: A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction

TitleA genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction
Authors
KeywordsBAC library
129Sv
Mouse genome
Gene targeting
Issue Date2005
Citation
Genomics, 2005, v. 86, n. 6, p. 753-758 How to Cite?
AbstractThe majority of gene-targeting experiments in mice are performed in 129Sv-derived embryonic stem (ES) cell lines, which are generally considered to be more reliable at colonizing the germ line than ES cells derived from other strains. Gene targeting is reliant on homologous recombination of a targeting vector with the host ES cell genome. The efficiency of recombination is affected by many factors, including the isogenicity (H. te Riele et al., 1992, Proc. Natl. Acad. Sci. USA 89, 5128-5132) and the length of homologous sequence of the targeting vector and the location of the target locus. Here we describe the double-end sequencing and mapping of 84,507 bacterial artificial chromosomes (BACs) generated from AB2.2 ES cell DNA (129S7/SvEvBrd-Hprt b-m2 ). We have aligned these BACs against the mouse genome and displayed them on the Ensembl genome browser, DAS: 129S7/AB2.2. This library has an average insert size of 110.68 kb and average depth of genome coverage of 3.63- and 1.24-fold across the autosomes and sex chromosomes, respectively. Over 97% of the mouse genome and 99.1% of Ensembl genes are covered by clones from this library. This publicly available BAC resource can be used for the rapid construction of targeting vectors via recombineering. Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129-derived ES cell lines. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/249011
ISSN
2023 Impact Factor: 3.4
2023 SCImago Journal Rankings: 0.850
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorAdams, David J.-
dc.contributor.authorQuail, Michael A.-
dc.contributor.authorCox, Tony-
dc.contributor.authorVan Der Weyden, Louise-
dc.contributor.authorGorick, Barbara D.-
dc.contributor.authorSu, Qin-
dc.contributor.authorChan, Wei In-
dc.contributor.authorDavies, Rob-
dc.contributor.authorBonfield, James K.-
dc.contributor.authorLaw, Frances-
dc.contributor.authorHumphray, Sean-
dc.contributor.authorPlumb, Bob-
dc.contributor.authorLiu, Pentao-
dc.contributor.authorRogers, Jane-
dc.contributor.authorBradley, Allan-
dc.date.accessioned2017-10-27T05:58:52Z-
dc.date.available2017-10-27T05:58:52Z-
dc.date.issued2005-
dc.identifier.citationGenomics, 2005, v. 86, n. 6, p. 753-758-
dc.identifier.issn0888-7543-
dc.identifier.urihttp://hdl.handle.net/10722/249011-
dc.description.abstractThe majority of gene-targeting experiments in mice are performed in 129Sv-derived embryonic stem (ES) cell lines, which are generally considered to be more reliable at colonizing the germ line than ES cells derived from other strains. Gene targeting is reliant on homologous recombination of a targeting vector with the host ES cell genome. The efficiency of recombination is affected by many factors, including the isogenicity (H. te Riele et al., 1992, Proc. Natl. Acad. Sci. USA 89, 5128-5132) and the length of homologous sequence of the targeting vector and the location of the target locus. Here we describe the double-end sequencing and mapping of 84,507 bacterial artificial chromosomes (BACs) generated from AB2.2 ES cell DNA (129S7/SvEvBrd-Hprt b-m2 ). We have aligned these BACs against the mouse genome and displayed them on the Ensembl genome browser, DAS: 129S7/AB2.2. This library has an average insert size of 110.68 kb and average depth of genome coverage of 3.63- and 1.24-fold across the autosomes and sex chromosomes, respectively. Over 97% of the mouse genome and 99.1% of Ensembl genes are covered by clones from this library. This publicly available BAC resource can be used for the rapid construction of targeting vectors via recombineering. Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129-derived ES cell lines. © 2005 Elsevier Inc. All rights reserved.-
dc.languageeng-
dc.relation.ispartofGenomics-
dc.subjectBAC library-
dc.subject129Sv-
dc.subjectMouse genome-
dc.subjectGene targeting-
dc.titleA genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.ygeno.2005.08.003-
dc.identifier.pmid16257172-
dc.identifier.scopuseid_2-s2.0-28644432881-
dc.identifier.volume86-
dc.identifier.issue6-
dc.identifier.spage753-
dc.identifier.epage758-
dc.identifier.eissn1089-8646-
dc.identifier.isiWOS:000234396200014-
dc.identifier.issnl0888-7543-

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