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- Publisher Website: 10.1038/ng788
- Scopus: eid_2-s2.0-0036338128
- PMID: 11740496
- WOS: WOS:000173105600015
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Article: Efficient Cre-loxP-induced mitotic recombination in mouse embryonic stem cells
Title | Efficient Cre-loxP-induced mitotic recombination in mouse embryonic stem cells |
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Authors | |
Issue Date | 2002 |
Citation | Nature Genetics, 2002, v. 30, n. 1, p. 66-72 How to Cite? |
Abstract | FLP/FRT-induced mitotic recombination provides a powerful method for creating genetic mosaics in Drosophila and for discerning the function of recessive genes in a heterozygous individual. Here we show that mitotic recombination can be reproducibly induced in mouse embryonic stem (ES) cells, by Cre/loxP technology, at frequencies ranging from 4.2 Ã 10 -5 (Snrpn) to 7.0 Ã 10 -3 (D7Mit178) for single allelic loxP sites, and to 5.0 Ã 10 -2 (D7Mit178) for multiple allelic lox sites, after transient Cre expression. Notably, much of the recombination occurs in G2 and is followed by X segregation, where the recombinant chromatids segregate away from each other during mitosis. It is X segregation that is useful for genetic mosaic analysis because it produces clones of homozygous mutant daughter cells from heterozygous mothers. Our studies confirm the predictions made from studies in Drosophila 1 that suggest that X segregation will not be limited to organisms with strong mitotic pairing, because the forces (sister-chromatid cohesion) responsible for X segregation are an elemental feature of mitosis in all eukaryotes. Our studies also show that genetic mosaic analysis in mice is feasible, at least for certain chromosomal regions. |
Persistent Identifier | http://hdl.handle.net/10722/249001 |
ISSN | 2023 Impact Factor: 31.7 2023 SCImago Journal Rankings: 17.300 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Liu, Pentao | - |
dc.contributor.author | Jenkins, Nancy A. | - |
dc.contributor.author | Copeland, Neal G. | - |
dc.date.accessioned | 2017-10-27T05:58:50Z | - |
dc.date.available | 2017-10-27T05:58:50Z | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | Nature Genetics, 2002, v. 30, n. 1, p. 66-72 | - |
dc.identifier.issn | 1061-4036 | - |
dc.identifier.uri | http://hdl.handle.net/10722/249001 | - |
dc.description.abstract | FLP/FRT-induced mitotic recombination provides a powerful method for creating genetic mosaics in Drosophila and for discerning the function of recessive genes in a heterozygous individual. Here we show that mitotic recombination can be reproducibly induced in mouse embryonic stem (ES) cells, by Cre/loxP technology, at frequencies ranging from 4.2 Ã 10 -5 (Snrpn) to 7.0 Ã 10 -3 (D7Mit178) for single allelic loxP sites, and to 5.0 Ã 10 -2 (D7Mit178) for multiple allelic lox sites, after transient Cre expression. Notably, much of the recombination occurs in G2 and is followed by X segregation, where the recombinant chromatids segregate away from each other during mitosis. It is X segregation that is useful for genetic mosaic analysis because it produces clones of homozygous mutant daughter cells from heterozygous mothers. Our studies confirm the predictions made from studies in Drosophila 1 that suggest that X segregation will not be limited to organisms with strong mitotic pairing, because the forces (sister-chromatid cohesion) responsible for X segregation are an elemental feature of mitosis in all eukaryotes. Our studies also show that genetic mosaic analysis in mice is feasible, at least for certain chromosomal regions. | - |
dc.language | eng | - |
dc.relation.ispartof | Nature Genetics | - |
dc.title | Efficient Cre-loxP-induced mitotic recombination in mouse embryonic stem cells | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1038/ng788 | - |
dc.identifier.pmid | 11740496 | - |
dc.identifier.scopus | eid_2-s2.0-0036338128 | - |
dc.identifier.volume | 30 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 66 | - |
dc.identifier.epage | 72 | - |
dc.identifier.isi | WOS:000173105600015 | - |
dc.identifier.f1000 | 1003169 | - |
dc.identifier.issnl | 1061-4036 | - |